Monoclonal antibodies defining immunogenic regions of pili from <i>Bacteroides nodosus</i> strains 198 (A1), 265 (H1) and 336 (F1)

Young, Deborah; Emery, D.L.; Dj, Stewart

doi:10.1038/icb.1989.9

Cited by 12 publications

(2 citation statements)

References 18 publications

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“…The current method of grouping B. nodosus is based on antigenic epitopes located on the surface fimbriae (24,31). Agglutination (slide or tube) or enzyme-linked immunosorbent assay (ELISA) is currently used to identify unknown strains (3,5,16,28).…”

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Polymerase chain reaction amplification of the constant and variable regions of the Bacteroides nodosus fimbrial gene

et al. 1990

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Serogrouping of Bacteroides nodosus is based on antigenic differences in fimbriae of the different New Zealand prototype strains. Because of the time needed to isolate and grow pure cultures of B. nodosus and the difficulty in distinguishing between different serogroups because of cross-agglutination, a new DNA-based diagnostic approach based on the fimbrial gene sequence of B. nodosus was developed. Published nucleotide sequences of the fimbrial genes for serogroups A, G, D, and H showed conservation at the 5' end, coding for the N terminus, and variability at the 3' end, coding for the C terminus. The polymerase chain reaction was used to amplify both the constant and variable regions of the fimbrial genes. Constant-region oligonucleotide primers were used to amplify a 100-base-pair fragment from the constant regions of the fimbrial genes of 10 New Zealand serogroups. Serogroup-specific oligonucleotide primers for serogroups A and H allowed amplification of a 282-base-pair fragment from serogroup A and a 363-base-pair fragment from serogroup H. Thus, amplification of the constant and variable regions of the fimbrial gene allows rapid detection and grouping of B. nodosus. Bacteroides nodosus is the causative agent of foot rot in sheep (1, 7). Foot rot remains a constant economic burden for many Western United States sheep ranchers. Serious cases of foot rot result in lameness, poor wool production, and susceptibility to other infectious diseases. The economic impact in the past few years has been substantial.

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Polymerase chain reaction amplification of the constant and variable regions of the Bacteroides nodosus fimbrial gene

et al. 1990

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“…For D. nodosus , fimbrial antigens are highly immunogenic, and fimbrial subunits of all nine major serogroups are well characterized for amino‐acid sequence (M ckern et al 1989; M attick et al 1991). Thus it is possible that multivalent D. nodosus vaccines may become a very useful model in understanding the genetic restrictions of protective antibody responses to multivalent vaccines (E mery et al 1984; Y oung et al 1989; S tewart et al 1991a; O'M eara et al 1993).…”

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Disease resistance in Merino sheep. V. Genetic heterogeneity in response to vaccination with Dichelobacter nodosus and clostridial antigens

Raadsma

Attard

Nicholas

et al. 1996

J Animal Breeding Genetics

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Summary Genetic aspects of antibody responses in two commercial, multivalent vaccines were examined in 2 974 Merino sheep, representing the progeny from 129 sires in four major bloodlines. All lambs were vaccinated twice, between 2 and 4 months of age, with a clostridial vaccine containing antigens against five clostridial species. Approximately half (1 239) the progeny from all sires were also vaccinated at 16 months of age, with a Dichelobacter nodosus vaccine containing whole‐cell antigens from all nine major serogroups (A–I). ELISA antibody titres to Clostridium tetani and Clostridium chauvoei were measured 2 weeks after booster vaccination. K‐agglutinating antibody titres, against all serogroups of D. nodosus, were measured on three occasions–following primary vaccination (V1), 3 and 8 weeks following booster vaccination (V2). Mean antibody titre on all three occasions (MEAN POST‐VAC), and changes in antibody titre between V1 and V2, and after V2, were analysed as additional antibody traits for D. nodosus. For both clostridial and D. nodosus antibody traits, year of birth and flock were major fixed effects. Sex of the lamb was not important in the case of clostridial antibody titres, but ewe hoggets had consistently higher D. nodosus antibody titres compared to those for ram hoggets from the same cohort. Based on restricted maximum likelihood (reml) analyses, heritability estimates for C. tetani and C. chauvoei were 0.12 (± 0.04) and 0.24 (± 0.07). Preliminary estimates for maternal effects showed additive maternal genetic variance for C. tetani and C. chauvoei (0.09 ± 0.05 and 0.17 ± 0.07). Maternal environmental effects appeared negligible. Heritability estimates of D. nodosus antibody titre for all nine serogroups ranged from 0.22 to 0.66 following V1, and were approximately 40% higher than those following V2 (range 0.17–0.44), which in turn were approximately 50% higher than estimates for persistence of titre (range 0.08–0.32). Genetic correlations for antibody titre at consecutive samplings were high, and the heritability estimates for MEAN POST‐VAC titre for each serogroup of D. nodosus were: A (0.51), B (0.58), C (0.41), D (0.24), E (0.34), F (0.33), G (0.26), H (0.34), and I (0.35). Genetic correlations for antibody titres among the nine D. nodosus serogroups ranged from moderately negative (–0.40) to strongly positive (0.87). Similarly, genetic correlations between clostridial and D. nodosus antibody titres ranged from –0.08 to 4–0.41. C. tetani antibody titre showed a genetic correlation of +0.50 with C. chauvoei titre. It was concluded that significant additive genetic variation exists in Merino sheep for vaccine response to commercial vaccines. However, there was no strong evidence for common genetic control of immune response to different antigens. Zusammenfassung Krankheitsresistenz bei Merinoshafen. V. Genetische Heterogenität der Reaktion auf Vakzine mit Dichelobacter nodosus und Klostidienantigene Genetische Aspekte de Antikörperreaktion auf zwei komerzielle multivalente Vakzinen wurden an 2...

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Humoral responses to a multivalent vaccine in age-matched lambs of different bodyweight and nutrition

Whittington

Edwards

Nicholls

et al. 1992

Research in Veterinary Science

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Monoclonal antibodies defining immunogenic regions of pili from Bacteroides nodosus strains 198 (A1), 265 (H1) and 336 (F1)

Cited by 12 publications

References 18 publications

Polymerase chain reaction amplification of the constant and variable regions of the Bacteroides nodosus fimbrial gene

Polymerase chain reaction amplification of the constant and variable regions of the Bacteroides nodosus fimbrial gene

Disease resistance in Merino sheep. V. Genetic heterogeneity in response to vaccination with Dichelobacter nodosus and clostridial antigens

Humoral responses to a multivalent vaccine in age-matched lambs of different bodyweight and nutrition

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