Monoclonal antibodies in immunodiagnostic assays: a review of recent applications

Mahmuda, Aliyu; Bande, Faruku; Abdulhaleem, Noor; Al-Zihiry, Khalid Jameel Kadhim; Majid, Roslaini Abd; Hamat, Rukman Awang; Abdullah, Wan Omar; Zasmy, Ngah

doi:10.4314/sokjvs.v15i1.1

Cited by 8 publications

(7 citation statements)

References 27 publications

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“…Following oral administration of prednisolone, a significant increase in egg per gram of feces was noted as well as yield in larvae. This observation is similar to earlier reports by Mahmuda et al (2017) and Ohnishi et al (2004) that used oral prednisolone to induce immunosuppression following S. ratti infection and the high number of S. stercoralis eggs recorded from AIDS patient respectively. Molecular analysis of the DNA obtained following experimental infection revealed similar morphological features of different stages of S. ratti and the phylogenetic analysis also show that S. ratti isolated was 97% similar to S. ratti and S. stercoralis.…”

Section: Discussionsupporting

confidence: 92%

Establishment Of Chronic Strongyloides ratti Infection Model And Its Diagnostic Significance

2019

University of Thi-Qar Journal of Medicine

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Infection with Strongyloides stercoralis is associated with asymptomatic chronic disease involving the gut and respiratory systems and can persist for long period undetected leading to development of fatal hyperinfection syndrome. The severity is even more in immunocompromised individuals or patients undergoing immune suppressive therapy. Unfortunately the disease is difficult to diagnose due to low parasite load and the irregular larval output. Development of efficient diagnostic techniques requires large supply of infective larvae and this can be achieved through in vivo maintenance of the parasite. In this regard, S. ratti is being used as a model instead of S. stercoralis due to difficulty and risk of infection. In this study, a significant amount of previously characterized filariform (iL3) larvae of S. ratti was collected. Chronic infection was successfully established and maintained by experimentally induced immunosuppression of Wistar rats following oral administration of prednisolone. This model can serve as a good source of infective larvae for production of monoclonal antibodies and development of diagnostic assays for prevention of the disease. It can also provide room for further research in diagnostics of human strongyloidiasis.

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Section: Discussionsupporting

confidence: 92%

Establishment Of Chronic Strongyloides ratti Infection Model And Its Diagnostic Significance

2019

University of Thi-Qar Journal of Medicine

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“…In the present study, we produced a polyclonal antibody of Sumateran wild boar meat as an immunodiagnostic reagent. In general, monoclonal antibodies were the main choice for immunodiagnostic reagents [18]; however, polyclonal antibodies has several advantages such as high affinity, able to identify high homologous proteins of immunogen proteins, can be used to filter protein targets of non-immunogen species, able to detect denatured proteins, and able to detect more than one epitope so the detection is more sensitive. To produce an antibody of Sumateran wild boar meat, we immunized rabbits with initial immunization and 3 times booster.…”

Section: Resultsmentioning

confidence: 99%

Sumateran wild boar (Sus scrofa vittatus) meat antibody production as immunodiagnostic reagent candidate

Adiningsih

Soejoedono

Adji³

et al. 2019

Vet World

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Aim: Meat authentication gives significance values in view of religious, food safety, public health, quality assurance, and legal concern. Most of the meat authentication is based on molecular assay; a simpler method to authenticate meat is needed to develop. An immunoassays technique may offer a solution for simpler test. The aim of our current study was to develop a polyclonal antibody of Sus scrofa vittatus (Sumateran wild boar) as an immunodiagnostic reagent candidate. Materials and Methods: Three male New Zealand white rabbits were used in this study for antibody production. Antigen used was meat extract of Sumateran wild boar, each rabbit was immunized with meat extract antigen (0.5 mg/ml) emulsified in Freund's complete adjuvant at a 1:1 (v/v) ratio as much as 1 ml at subcutaneous route. Booster was carried out 3 times with interval time of 14 days, using meat extract antigen emulsified in Freund's incomplete adjuvant at a 1:1 (v/v) ratio. Serum samples were taken every week, start from 1 week after the first immunization up to 1 week after the third booster. Antibody purification was performed using ammonium sulfate precipitation and Protein A. The presence of specific antibody was determined using agar gel precipitation test and enzyme-linked immunosorbent assay, while purified specific IgG was characterized using sodium dodecyl sulfate-polyacrylamide gel electrophoresis method. Results: Specific antibody was detected at 14 days after the first immunization and still detected until 2 weeks after the third booster. Highest absorbance of specific antibody was detected 1 week after the third booster. Conclusion: The present study demonstrated that specific antibody of Sumateran wild boar is favorable to be produced in rabbit and showed that antibody produced is applicable to detect Sumateran wild boar meat antigen in immunodiffusion assay, indicating that it is promising as a reagent candidate in immunodiagnostic assay/kit.

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“…Monoclonal antibodies play an inevitable role in the development of diagnostics (Mahmuda et al, 2017). These single-epitope antibodies have high efficiency and sensitivity in detecting viral proteins, viruses, and other antigenic substances (Gubler et al, 1984;Gerna et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Immuno-dominant dengue NS1 peptides as antigens for production of monoclonal antibodies

Munasinghe

Athapaththu²,

Abeyewickreme³

2022

Front. Mol. Biosci.

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Synthetic peptides have recently become common as antigens for antibody production. Peptides are short chains of amino acids that can be used to elicit an immune response. The immunogenicity of the peptide antigens varies depending on the length, charge, solubility, and amino acids contained in the peptide sequence. Dengue NS1 protein is an important target antigen in the early detection of dengue infection. In this study, peptides corresponding to a highly conserved region from the dengue NS1 region were designed and synthesized. Balb/C mice were immunized against each peptide and spleen cells extracted from the immunized mice were fused with NS0 murine myeloma cells. Hybridoma clones obtained from the fusions were tested against peptides using ELISA. Out of 1,830 growing clones, 28 clones produced antibodies reacting with dengue NS1 peptides. A purified monoclonal antibody reacting with all four peptides was tested for reactivity with dengue NS1 native protein using dengue-confirmed serum and urine samples. The monoclonal antibody shows significant reactivity with both serum and urine. The findings of the current research can be used to detect dengue infection using urine, which ultimately results in the prevention of dengue epidemics through painless diagnosis, following treatment, and patient management to safeguard human and economic wellness.

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Monoclonal antibodies in immunodiagnostic assays: a review of recent applications

Cited by 8 publications

References 27 publications

Establishment Of Chronic Strongyloides ratti Infection Model And Its Diagnostic Significance

Establishment Of Chronic Strongyloides ratti Infection Model And Its Diagnostic Significance

Sumateran wild boar (Sus scrofa vittatus) meat antibody production as immunodiagnostic reagent candidate

Immuno-dominant dengue NS1 peptides as antigens for production of monoclonal antibodies

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