Monoclonal antibodies raised against K562 cells reacted with human haematopoietic pluripotent stem cells

Kubota, Yoshihiro; Tanaka, Terukazu; Irino, Shozo

doi:10.1016/0145-2126(91)90121-9

Cited by 1 publication

(1 citation statement)

References 17 publications

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“…Furthermore, monoclonal antibodies against K562 cells also recognize human haematopoietic pluripotent stem cells. 54 Our findings of concordant age-related epigenomic associations across multiple cell types confirm previous studies that reported a high correlation of the age-associated loci in multiple tissues and cell types. 7,14,17 The consistency of the epigenomic signature shared by different sets of aDMRs and aGENs cannot be attributed solely to their overlapping genomic locations.…”

Section: Discussionsupporting

confidence: 81%

Polycomb repressive complex 2 epigenomic signature defines age-associated hypermethylation and gene expression changes

Dozmorov

2015

Epigenetics

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Although age-associated gene expression and methylation changes have been reported throughout the literature, the unifying epigenomic principles of aging remain poorly understood. Recent explosion in availability and resolution of functional/regulatory genome annotation data (epigenomic data), such as that provided by the ENCODE and Roadmap Epigenomics projects, provides an opportunity for the identification of epigenomic mechanisms potentially altered by age-associated differentially methylated regions (aDMRs) and regulatory signatures in the promoters of ageassociated genes (aGENs). In this study we found that aDMRs and aGENs identified in multiple independent studies share a common Polycomb Repressive Complex 2 signature marked by EZH2, SUZ12, CTCF binding sites, repressive H3K27me3, and activating H3K4me1 histone modification marks, and a "poised promoter" chromatin state. This signature is depleted in RNA Polymerase II-associated transcription factor binding sites, activating H3K79me2, H3K36me3, H3K27ac marks, and an "active promoter" chromatin state. The PRC2 signature was shown to be generally stable across cell types. When considering the directionality of methylation changes, we found the PRC2 signature to be associated with aDMRs hypermethylated with age, while hypomethylated aDMRs were associated with enhancers. In contrast, aGENs were associated with the PRC2 signature independently of the directionality of gene expression changes. In this study we demonstrate that the PRC2 signature is the common epigenomic context of genomic regions associated with hypermethylation and gene expression changes in aging.

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Section: Discussionsupporting

confidence: 81%

Polycomb repressive complex 2 epigenomic signature defines age-associated hypermethylation and gene expression changes

Dozmorov

2015

Epigenetics

View full text Add to dashboard Cite

show abstract

Monoclonal antibodies raised against K562 cells reacted with human haematopoietic pluripotent stem cells

Cited by 1 publication

References 17 publications

Polycomb repressive complex 2 epigenomic signature defines age-associated hypermethylation and gene expression changes

Polycomb repressive complex 2 epigenomic signature defines age-associated hypermethylation and gene expression changes

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