1986
DOI: 10.1530/jrf.0.0780663
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Monoclonal antibodies recognize a cell surface marker of epithelial differentiation in the rabbit reproductive tract

Abstract: Summary. Monoclonal antibodies against the cell surface were produced by immunizing mice with endometrial scrapings prepared from 6-day pregnant rabbits. Spleen cells from an immune mouse were fused with myeloma cells and cultured by standard hybridoma technology methods. Hybridoma supernatants were screened for reaction with the apical epithelial surface by immunohistochemistry on frozen sections of uterus from 6-day pregnant rabbits, and positive colonies were cloned by limiting dilution. Ascites fluid was p… Show more

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Cited by 5 publications
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