Monoclonal antibodies recognizing epitopes of calretinins: dependence on Ca2+-binding status and differences in antigen accessibilityin colon cancer cells

The Ca2+-binding protein calretinin is selectively enriched in a subpopulation of the epithelial rests of Malassez

“…The specificity of the polyclonal (Schwaller et al 1993) and monoclonal (Zimmermann and Schwaller 2002) calretinin antibodies, the monoclonal parvalbumin antibody (Celio et al 1988), and the monoclonal calbindin D28k antibody (Celio et al 1990) was confirmed by immunoblot analyses.…”

“…Adult ERM, which have previously been described as containing calbindin D28k (Onishi et al 1999(Onishi et al , 2003 possibly also contains calretinin. The rabbit polyclonal (Schwaller et al 1993) and mouse monoclonal (Zimmermann and Schwaller 2002) anti-calretinin antibodies that we have used in our study react specifically with calretinin and do not cross-react with calbindin D28k. In addition, the mouse monoclonal calbindin D28k antibody that has been used in the present study does not cross-react with calretinin (Celio et al 1990).…”

Mediodorsal thalamic afferents to layer III of the rat prefrontal cortex: Synaptic relationships to subclasses of interneurons

“…As mentioned in the Material and Methods section, the specificity of the antibodies used in this study was previously tested and proved to be reliable (Celio, 1986;Celio et al, 1988;Celio and Heizmann, 1981;Conde et al, 1994;Rogers, 1987;Schwaller et al, 1993;Sesack et al, 1995a;Sesack et al, 1998;Zimmermann and Schwaller, 2002). Still in the manufacturer immunoblot control test, it was estimated that the polyclonal rabbit anti-CalB antibody crossreacted by 10% with CalR.…”

“…These tests involved preadsorbtion controls, Western blot analysis, or radioimmunoassay (Celio, 1986;Celio et al, 1988;Celio and Heizmann, 1981;Conde et al, 1994;Rogers, 1987;Schwaller et al, 1993;Zimmermann and Schwaller, 2002). The specificity of some of these antibodies (the polyclonal rabbit anti-ParV and rabbit anti-CalR) is further supported by the absence of staining when these antibodies were omitted (Sesack et al, 1995a;Sesack et al, 1998).…”

The densities of calbindin and parvalbumin, but not calretinin neurons, are sexually dimorphic in the amygdala of the guinea pig

“…The specificity of the primary antisera used in this study has been shown by the manufacturers and various researchers using these products in multiple previous studies (Airaksinen et al, 1997;Drexel et al, 2011;Ellis and Mawe, 2003;Meszar et al, 2012;Woodruff and Sah, 2007a;Zimmermann and Schwaller, 2002). Products descriptions of rabbit anticalbindin (CB-38) and mouse anti-calretinin (6B 3 ) antisera include immunoblots of the guinea pig brain homogenates, which were specifically stained by these antibodies, showing bands at 28 kDa and 29 kDa, respectively.…”