Monoclonal antibodies to beet soil‐borne virus

Abstract

Four monoclonal antibodies (MCA) were obtained to the 'Ahlum' serotype of beet soil-borne virus (BSBV). On ELlSA plates which had been precoated with polyclonal antibodies (PCA) all four MCA detected this serotype with a higher sensitivity than alkaline phosphatase-labelled PCA. Three of the MCA were specific for the 'Ahlum' serotype, but a fourth one also detected the distantly related 'Wierthe' serotype. Cross-reactions with wheat soil-borne or oat golden stripe furoviruses were not observed. One of the MCA reacted with an epitope which is exposed along the entire length of the BSBV particles, whereas two others were specific for epitopes which are exposed on one particle extremity only. Although these latter two epitopes occur apparently on the same extremity of the particles, they seem to be different, because one is found only on the particles of the 'Ahlum' serotype, whereas the other one is present also on the particles of the 'Wierthe' serotype. The fourth MCA is specific for a cryptotope which is not exposed on the intact virus particles, but presumably on some degradation product or precursor of the viral coat protein present in crude sap preparations. All four epitopes are SDS-labile; they are not detected on denatured viral coat protein on Western blots.

RNA silencing machinery contributes to inability of BSBV to establish infection in Nicotiana benthamiana: evidence from characterization of agroinfectious clones of Beet soil-borne virus

“…1d), suggesting an efficient production of virions by the viral clones. Notably, some of the particles showed a tendency to associate together and form bundles, as already reported for previous studies on BSBV virions [2].…”

“…Beet soil-borne virus (BSBV) is a single-stranded, positive-sense ((+)ss) RNA virus that naturally infects sugar beet ( Beta vulgaris ), putatively using the plasmodiophorid Polymyxa betae (a protist parasite from the supergroup Rhizaria ) as vector [1]. The BSBV genome is divided into three RNA segments (RNA 1–3) that are encapsidated into rod-shaped particles [2]. RNA 1 is 5.8 kb in length and encodes the replication proteins [3].…”

Genome properties of beet virus Q, a new furo-like virus from sugarbeet, determined from unpurified virus.

“…related strain of BSBV (Lesemann et al, 1989 ;Barbarossa et al, 1992), but the molecular data presented in this paper suggest that it is a distinct virus for which we propose the name beet virus Q (BVQ). A starter sequence, which was generated with primers specific for highly conserved helicase-encoding domains in RNA 1 of furo-, pomo-, peclu-, hordei-and tobraviruses (Koenig & Loss, 1997), has enabled the entire nucleotide sequence of the tripartite genome of unpurified BVQ to be determined.…”

The sugar beet multi-talented resistance protein Rz2

“…Both viruses are present in mixed infection with BNYVV and at least BSBV is distributed in sugar beet fields throughout the world (Mahillon et al 2021). Members of the genus Pomovirus have a tripartite RNA genome of about 6 kbp, 3-3.5 kbp and 2-2.5 kbp in size and are organized in rod-shaped segments (Barbarossa et al 1992). RNA1 encodes two readthrough proteins involved in replication (Barbarossa et al 1992).…”

“…Members of the genus Pomovirus have a tripartite RNA genome of about 6 kbp, 3-3.5 kbp and 2-2.5 kbp in size and are organized in rod-shaped segments (Barbarossa et al 1992). RNA1 encodes two readthrough proteins involved in replication (Barbarossa et al 1992). The CP and its CP-RT are encoded on RNA2, whereas the overlapping ORFs on RNA3 encode the TGB proteins (TGB1, TGB2 2 and TGB3) responsible for cell-to-cell movement (Koenig et al 1996;Koenig et al 1997a).…”