Monoclonal antibodies to cytoskeletal proteins: An immunohistochemical investigation of human colon cancer

Rm, Porter; Tc, Holme; El, Newman; Hopwood, David A.; Jm, Wilkinson; Cuschieri, Alfred

doi:10.1002/path.1711700406

Cited by 34 publications

(26 citation statements)

References 25 publications

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“…22) The detransforming activity and induction of gelsolin by KBH-A145 is supported by previous reports that most HDACIs, including depudecin, FR901228, trapoxin, oxamflatin, apicidin, and TSA, induce morphological changes of v-ras-transformed NIH3T3 cells and HeLa cells 12,18,19,22) and that malignant transformation correlated with decreased expression of gelsolin. 23) Furthermore, given that accumulation of hyperacetylated histone H3 or H4 was seen after treatment with g-lactam-based hydroxamic acid in MDA-MB-231 and ACHN cells (Fig. 2B), changes in the levels of p21…”

Section: Resultsmentioning

confidence: 91%

A Novel .GAMMA.-Lactam-Based Histone Deacetylase Inhibitor Potently Inhibits the Growth of Human Breast and Renal Cancer Cells

Kwon

Ahn

Park

et al. 2009

Biological & Pharmaceutical Bulletin

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Section: Resultsmentioning

confidence: 91%

A Novel .GAMMA.-Lactam-Based Histone Deacetylase Inhibitor Potently Inhibits the Growth of Human Breast and Renal Cancer Cells

Kwon

Ahn

Park

et al. 2009

Biological & Pharmaceutical Bulletin

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“…To our knowledge, there has been one English publication concerning immunohistochemical analysis for caldesmon expression in stromal cells of human colon carcinomas (21). In that study, myofibroblastic stromal cells were also positive for caldesmon, because the antibody to caldesmon used in that work recognized not only h-CD but also low-molecular-weight caldesmon (I-CD), and the latter molecule is expressed in both non-muscle cells and smooth muscle cells (22).…”

Section: Discussionmentioning

confidence: 99%

The Role of Myofibroblasts at the Tumor Border of Invasive Colorectal Adenocarcinomas

Nakayama

Enzan

Miyazaki

et al. 1998

Japanese Journal of Clinical Oncology

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Background:In order to elucidate the significance of myofibroblasts in invasive growth of colorectal adenocarcinomas, we examined the number of myofibroblasts at the tumor border of colorectal adenocarcinomas.Method: A total of 91 invasive colorectal adenocarcinomas were examined immunohistochemically using anti-alpha-smooth muscle actin (ASMA) and high-molecular-weight caldesmon (h-CD) antibodies; 25 carcinomas confined to the submucosa (sm carcinomas), 40 carcinomas confined to the muscularis propria (mp carcinomas) and 26 carcinomas invading the subserosa or adventitia (ss carcinomas). We considered ASMA-positive and h-CD-negative stromal cells as myofibroblasts.Results: Twenty-seven (67%) of the 40 mp carcinomas and 25 (96%) of the 26 ss carcinomas had a small number of myofibroblasts at the tumor border facing the muscularis propria. Conclusions:Although direct evidence is lacking, there is a possibility that the further immediately vertical and radial invasion of carcinoma cells into the subserosa or adventitia is associated with a smaller number of myofibroblasts at the tumor border facing the muscularis propria in mp carcinomas, resulting in a low incidence of mp and a high incidence of ss carcinomas in the colorectum.

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“…Gelsolin, a protein which is highly conserved in vertebrates, controls the length of actin ®laments in vitro and the cell shape and motility in vivo by a variety of mechanisms (Cunningham et al, 1991). A signi®cant decrease in the amount of gelsolin is associated with malignant transformation (Chaponnier and Gabbiani, 1989;Moriya et al, 1994;Porter et al, 1993;Vandekerckhove et al, 1990). Gelsolin is one of the most strikingly down-regulated markers of the (Tanaka et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Oxamflatin is a novel antitumor compound that inhibits mammalian histone deacetylase

et al. 1999

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Oxam¯atin [(2E) -5 -[3 -[(phenylsufonyl) amino] phenyl]-pent-2-en-4-ynohydroxamic acid] induces transcriptional activation of junD and morphological reversion in various NIH3T3-derived transformed cell lines. We found that oxam¯atin showed in vitro antiproliferative activity against various mouse and human tumor cell lines with drastic changes in the cell morphology and in vivo antitumor activity against B16 melanoma. Oxam¯atin caused an elongated cell shape with ®lamentous protrusions as well as arrest of the cell cycle at the G1 phase in HeLa cells. These phenotypic changes of HeLa cells were apparently similar to those by trichostatin A (TSA), a speci®c inhibitor of histone deacetylase (HDAC). The e ect of oxam¯atin on the transcriptional activity of the cytomegalovirus (CMV) promoter was examined and compared with known HDAC inhibitors, TSA, sodium n-butyrate, and FR901228. Oxam¯atin as well as all these inhibitors greatly enhanced the transcriptional activity of the CMV promoter in a dose-dependent manner. Oxam¯atin, like TSA, inhibited intracellular HDAC activity, as a result of which marked amounts of acetylated histone species accumulated. Finally, e ects on expression of several endogenous genes involved in cell morphology and cell cycle control in HeLa cells were analysed. Expression of gelsolin, cyclin E and Cdk inhibitors including p21 WAF1/Cip1 was highly augmented, while that of cyclin A and cyclin D1 was decreased by oxam¯atin. These results suggest that changes in the expression pattern of the genes regulating cell morphology and the cell cycle due to histone hyperacetylation are responsible for the antitumor activity, the morphological change and the cell cycle arrest induced by oxam¯atin.

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Monoclonal antibodies to cytoskeletal proteins: An immunohistochemical investigation of human colon cancer

Cited by 34 publications

References 25 publications

A Novel .GAMMA.-Lactam-Based Histone Deacetylase Inhibitor Potently Inhibits the Growth of Human Breast and Renal Cancer Cells

A Novel .GAMMA.-Lactam-Based Histone Deacetylase Inhibitor Potently Inhibits the Growth of Human Breast and Renal Cancer Cells

The Role of Myofibroblasts at the Tumor Border of Invasive Colorectal Adenocarcinomas

Oxamflatin is a novel antitumor compound that inhibits mammalian histone deacetylase

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