Monoclonal antibodies to oxytocin: production and characterization

Burgeon, Emmanuel; Chapleur, Michèle; Schoenen, Jean; Remichius, Didier; Legros, Jean-Jacques; Geenen, Vincent; Robert, Françoise

doi:10.1016/0165-5728(91)90045-9

Cited by 16 publications

(9 citation statements)

References 15 publications

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“…033 and 013 are anti-OT mAbs; 033 is directed against the cyclic part of OT and 013 is directed against the C-terminal part of OT [25]. BER 312 is an anti-VP mAb [26].…”

Section: Antibodiesmentioning

confidence: 99%

Cytokine production by human thymic epithelial cells: control by the immune recognition of the neurohypophysial self-antigen

Martens

Malgrange

Robert

et al. 1996

Regulatory Peptides

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Oxytocin (OT) has been shownto be the dominantpeptideof the neurohypophysial familyexpressedby thymicepithelialand nurse cells (TEC/TNC) invmiousspecies. Thymic OT is not secreted but, after translocation of a hybrid neurophysin/MHC class I protein,is integratedwithinthe plasmamembraneof TEC, thus allowing its presentation to pre-T cells. In order to further demonstrate that thymic OT behaves like a membrane antigen, we assessed the effect of rnAbs to OT on cytokine productions by cultures enriched in human TEC. 75-85% pure TEC cultures were prepared tlom human thymic fragments. Using immunofluorescence and confocal microscopy, ir-OT, ir-interleukin-1~(IL-1P), ir-interleukin-6 (IL-6) and ir-leukemia inhibitory factor (LIF) could be detected in these TEC cultures. ir-OT was restricted to TEC, while some ir-IL-6 and ir-LIF were also seen in occasional fibroblasts. In basal conditions, ir-IL-6 and ir-LIF (but not ir-OT and ir-IL-l@) were detected in the supematants of human TEC cultures. MAbs to OT induced a marked increase of ir-IL-6 and ir-LIF secretion in TEC cultures. No significant effect was observed using mAbs against vasopressin, mouse immunoglobulins, or control ascitic fluid controls. These data show that OT is fully processed and recognized by specific mAbs at the outer surface of TEC plasma membrane. They further support that thymic OT behaves as the self-antigen of the nenrohypophysial family.

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“…033 and 013 are anti-OT mAbs; 033 is directed against the cyclic part of OT and 013 is directed against the C-terminal part of OT [25]. BER 312 is an anti-VP mAb [26].…”

Section: Antibodiesmentioning

confidence: 99%

Cytokine production by human thymic epithelial cells: control by the immune recognition of the neurohypophysial self-antigen

Martens

Malgrange

Robert

et al. 1996

Regulatory Peptides

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“…Further, in human thymus, we showed that the stromal cells containing ir-neuropeptides corre-spond to a subset of epithelial cells expressing KL1-and KL4-defined cytokeratins (Robert et al, 1991). The epithelial nature of the cells containing NHP-related peptides was confirmed, in rodent thymuses, using an antiserum to human keratins and KL4 (data not shown).…”

Section: Evidence For Neurohypophysial-like Peptides In Thymic Extractsmentioning

confidence: 60%

“…The epithelial nature of the cells containing NHP-related peptides was confirmed, in rodent thymuses, using an antiserum to human keratins and KL4 (data not shown). However, KL1 only stained a few medullary TEC in rodents (Nicolas et al, 1989), whereas it strongly labels cortical and medullary TEC from humans (Robert et al, 1991), evidencing a certain disparity between rodent and human TEC.…”

Section: Evidence For Neurohypophysial-like Peptides In Thymic Extractsmentioning

confidence: 99%

“…Using double immunofluorescence staining with A/5/III, a rabbit serum anti-NPs, we found that the same cells express together ir-OT and ir-NP (Robert et al, 1991 (Robert et al, 1991).…”

Section: Evidence For Neurohypophysial-like Peptides In Thymic Extractsmentioning

confidence: 99%

“…On the other hand, it was suggested that ANP could be localized in thymocytes (Vollmar and Schulz, 1990). Furthermore, NT and SS immunoreactivities were found in sparse stromal cells (Sundler et al, 1978), whereas neurokinin A (NKA) (Ericsson et al, 1990), opioid peptides (Von Gaudecker et al, 1986;Piantelli et al, 1990), OT, VP, and NPs (Robert et al, 1991) were localized in thymic epithelial cells (TEC).…”

Section: Introductionmentioning

confidence: 99%

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The Recognition of Hypothalamo‐NeurohypophysialFunctions by Developing T Cells

Robert

Martens

Cormann

et al. 1992

Journal of Immunology Research

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KEYWORDS:Neuropeptide signals and specific neuropeptide receptors have been described in the thymus supporting the concept of a close dialogue between the neuroendocrine and the immune systems at the level of early T-cell differentiation. In this paper, we review recent data about neurohypophysial (NHP)-related peptides detected in the thymus from different species. We suggest that we are dealing in fact with other member(s) of the NHP hormone family, which seems to exert its activity locally through a novel model of cell-to-cell signaling, that of cryptocrine communication. This model involves exchange of signals between thymic epithelial cells and developing thymocytes. The NHP-related peptides have been shown to trigger thymocyte proliferation and could induce immune tolerance of this highly conserved neuroendocrine family.Oxytocin, vasopressin, thymic epithelium, neurohypophysial peptide receptors, self-recognition, cryptocrine signaling. INTRODUCTIONIn the last decade, a number of studies has been reported indicating that various neuropeptides could be found in the thymus or in thymic neoplasms. Thus, neurotensin (NT) (Sundler et al., 1978;Herbst et al., 1987), somatostatin (SS) (Sundler et al., 1978;Geppetti et al., 1987;Fuller and Verity, 1989;Gomariz et al., 1990), oxytocin (OT) (Geenen et al., 1986;Ervin et al., 1988; Argiolas et al., 1990a;Jevremovic et al., 1990), vasopressin (VP) (Markwick et al., 1986;Geenen et al., 1987;Giraud et al., 1990), neurophysins (NPs) (Geenen et al., 1986), tachykinins (TKs) (Geppetti et al., 1988;Weihe et al., 1989;Ericsson et al., 1990;Lorton et al., 1990;Piantelli et al., 1990), neuropeptide Y (NPY) (D'Andrea et al., 1989;Weihe et al., 1989;Ericsson et al., 1990), vasoactive intestinal peptide (VIP) (Felten et al., 1985;Gomariz et al., 1990;A1-Shawaf et al., 1991), calcitonin gene-related peptide (CGRP) (Geppetti et al., 1989;Weihe et al., 1989), opioid peptides (Von Gaudecker et al., 1986;Piantelli et al., 1990), corticotropin (ACTH) (Her.bst et al., 1987), cholecystokinin (CCK) (Herbst et al., 1987), and atrial *Corresponding author. natriuretic peptide (ANP) (Vollmar and Schulz, 1990) have been immunologically, biologically, biochemically, and/or molecularly evidenced. These neuropeptides were not systematically located in the thymic structures. However, some immunoreactive (ir-) neuropeptides were localized in nerve fibers: substance P (SP) (Geppetti et al., 1987(Geppetti et al., , 1988Weihe et al., 1989;Lorton et al., 1990), CGRP (Weihe et al., 1989), VIP (Felten et al., 1985;A1-Shawaf et al., 1991), NPY (D'Andrea et al., 1989;Weihe et al., 1989). On the other hand, it was suggested that ANP could be localized in thymocytes (Vollmar and Schulz, 1990). Furthermore, NT and SS immunoreactivities were found in sparse stromal cells (Sundler et al., 1978), whereas neurokinin A (NKA) (Ericsson et al., 1990), opioid peptides (Von Gaudecker et al., 1986;Piantelli et al., 1990), OT, VP, and NPs (Robert et al., 1991) were localized in thymic epithelial c...

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Subcellular localization of immunoreactive oxytocin within thymic epithelial cells of the male mouse

Wiemann

Ehret

1993

Cell Tissue Res

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Immunoreactive oxytocin is expressed by thymic epithelial cells, which share properties with neuroendocrine cells. In order to investigate the assumed paracrine secretion of oxytocin, we studied the subcellular localization of immunoreactive oxytocin within thymic tissue and cultured thymic epithelial cells of the male mouse. Three types of immunoreactive cells were distinguished with the electron microscope. Immunoreactive oxytocin was found to be restricted to the cytoplasm by the use of pre- and postembedding methods. Some epithelial cells, especially in the cortex, showed a pronounced labelling of vesicular membranes and membrane tubules of the endoplasmic reticulum. In some cells, keratin filaments were associated with the electron-dense stain. Under culture conditions immunoreactive cells of different shapes were found, all displaying similar patterns of labelling. The contents of different types of vacuoles were only rarely labelled. A special class of immunoreactive exocytotic vesicles could not be identified. Thus, our results do not support neuroendocrine secretion of oxytocin via vesicles of thymic epithelial cells but offer alternative modes of secretion.

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Monoclonal antibodies to oxytocin: production and characterization

Cited by 16 publications

References 15 publications

Cytokine production by human thymic epithelial cells: control by the immune recognition of the neurohypophysial self-antigen

Cytokine production by human thymic epithelial cells: control by the immune recognition of the neurohypophysial self-antigen

The Recognition of Hypothalamo‐NeurohypophysialFunctions by Developing T Cells

Subcellular localization of immunoreactive oxytocin within thymic epithelial cells of the male mouse

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