1996
DOI: 10.1007/bf02527716
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Monoclonal antibodies to proteins of the myelin-like sheath of earthworm giant axons show cross-reactivity to crayfish CNS glia: An immunogold electron microscopy study

Abstract: Monoclonal antibodies were generated to the proteins in myelin-like membranes isolated from the nerve cords of the earthworm, Lumbricus terrestris. One of these showing cross-reactivity to 30-32 and 40 kDa proteins was shown by immunofluorescence microscopy and immunogold electron microscopy to be bound primarily to glial cell process and their membranes and the myelin-like layers. This antibody cross-reacted with proteins of 60-65, 42, and 40 kDa in crayfish (Procambarus clarki) nerve cord homogenates. Locali… Show more

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Cited by 6 publications
(4 citation statements)
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“…Similarly, for several insect and crustacean species, myelin-like structures were described (light pink shading) and species in which myelin-like structures have not been described appear in red. This schematic representation is inspired and modified from Figure 3 myelin may share some molecular properties as monoclonal antibodies generated against earthworm myelin-like sheaths, cross-reacted with crayfish glia forming myelin-like structures (Cardone & Roots, 1996). Myelin, however, may also have different evolutionary origins.…”
Section: Evolution Of Myelinmentioning
confidence: 99%
“…Similarly, for several insect and crustacean species, myelin-like structures were described (light pink shading) and species in which myelin-like structures have not been described appear in red. This schematic representation is inspired and modified from Figure 3 myelin may share some molecular properties as monoclonal antibodies generated against earthworm myelin-like sheaths, cross-reacted with crayfish glia forming myelin-like structures (Cardone & Roots, 1996). Myelin, however, may also have different evolutionary origins.…”
Section: Evolution Of Myelinmentioning
confidence: 99%
“…Roots suggests that crayfish and crabs also have myelin [14], whilst Hartline and Colman include only prawns, shrimps, and copepods [38]. There is evidence that monoclonal antibodies to annelid myelin protein bind to crayfish CNS glia but no direct imaging evidence [39]. Other authors find no evidence for myelination in crayfish, instead arguing that they have lost myelin over the course of evolution-an intriguing possibility discussed further below [40].…”
Section: Arthropodsmentioning
confidence: 99%
“…The protein composition of invertebrate myelin is obscure. Some components have been identified on electrophoresis which appear to be unrelated to any of the vertebrate proteins [39,54], but almost nothing is known of their function. This is clearly an area where further research would be beneficial; we can speculate that a better understanding of the genetics of invertebrate myelination might shed light on why molluscs and insects have not developed myelin.…”
Section: Molecular Evidencementioning
confidence: 99%
“…These cytoplasmic brown bodies were seen as electron-dense particles using transmission electron microscopy (TEM). Previous ultrastructural analysis of Lumbricidae nerve tissue has concentrated on the major ganglia and axons of the ventral nerve cord (Levi et al 1966;AL-Yousuf et al 1992;Guenther 1976;Kensler et al 1979;Oesterle and Barth 1981;Roots et al 1991;Cardone and Roots 1996). However, very little is known about the ultrastructure of the neurons within the muscular body wall of L. terrestris.…”
Section: Introductionmentioning
confidence: 99%