Monoclonal antibodies to rhamnogalacturonan I backbone

Ralet, Marie-Christine; Tranquet, Olivier; Poulain, Daniel; Moïse, Adeline; Guillon, Fabienne

doi:10.1007/s00425-010-1116-y

Cited by 117 publications

(101 citation statements)

References 45 publications

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“…INRA-RU1 binds unbranched rhamnogalacturonan I chains (Ralet et al, 2010), while LM21 binds effectively to all HM polymers, regardless of their degree of substitution (Marcus et al, 2010). Wild-type and mutant mucilage showed a similar INRA-RU1 labeling (Fig.…”

Section: Muci10 Is Essential For the Distribution Of Hm In Adherent Mmentioning

confidence: 84%

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MUCILAGE-RELATED10 Produces Galactoglucomannan That Maintains Pectin and Cellulose Architecture in Arabidopsis Seed Mucilage

et al. 2015

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Plants invest a lot of their resources into the production of an extracellular matrix built of polysaccharides. While the composition of the cell wall is relatively well characterized, the functions of the individual polymers and the enzymes that catalyze their biosynthesis remain poorly understood. We exploited the Arabidopsis (Arabidopsis thaliana) seed coat epidermis (SCE) to study cell wall synthesis. SCE cells produce mucilage, a specialized secondary wall that is rich in pectin, at a precise stage of development. A coexpression search for MUCILAGE-RELATED (MUCI) genes identified MUCI10 as a key determinant of mucilage properties. MUCI10 is closely related to a fenugreek (Trigonella foenumgraecum) enzyme that has in vitro galactomannan a-1,6-galactosyltransferase activity. Our detailed analysis of the muci10 mutants demonstrates that mucilage contains highly branched galactoglucomannan (GGM) rather than unbranched glucomannan. MUCI10 likely decorates glucomannan, synthesized by CELLULOSE SYNTHASE-LIKE A2, with galactose residues in vivo. The degree of galactosylation is essential for the synthesis of the GGM backbone, the structure of cellulose, mucilage density, as well as the adherence of pectin. We propose that GGM scaffolds control mucilage architecture along with cellulosic rays and show that Arabidopsis SCE cells represent an excellent model in which to study the synthesis and function of GGM. Arabidopsis natural varieties with defects similar to muci10 mutants may reveal additional genes involved in GGM synthesis. Since GGM is the most abundant hemicellulose in the secondary walls of gymnosperms, understanding its biosynthesis may facilitate improvements in the production of valuable commodities from softwoods.

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Section: Muci10 Is Essential For the Distribution Of Hm In Adherent Mmentioning

confidence: 84%

“…Whole seeds were immunolabeled as described previously (Macquet et al, 2007) using LM21 (PlantProbes) and INRA-RU1 (Institut National de la Recherche Agronomique) primary antibodies (Marcus et al, 2010;Ralet et al, 2010). Alexa Fluor 488 (Molecular Probes, Life Technologies) was used as a secondary antibody.…”

Section: Immunolabeling Experimentsmentioning

confidence: 99%

MUCILAGE-RELATED10 Produces Galactoglucomannan That Maintains Pectin and Cellulose Architecture in Arabidopsis Seed Mucilage

et al. 2015

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“…Same as in flax [42,43], fraction of this polymer is so tightly retained by cellulose that can be obtained only after complete cellulose degradation [38]. The antibody RU2 against rhamnogalacturonan I backbone [44] does not recognize any epitopes within S1 layer, but binds to the thick inner cell wall layers of hemp fibers, both primary and secondary, same as to the primary cell wall/middle lamellae region [38] (Figure 6). Cytochemical staining for pectin is also positive in the inner layers of hemp fibers [6].…”

Section: Cell Wall Thickeningmentioning

confidence: 99%

Development of Hemp Fibers: The Key Components of Hemp Plastic Composites

Tatyana¹,

Mikshina²,

Vadim³

et al. 2018

Natural and Artificial Fiber-Reinforced Composites as Renewable Sources

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Plant fibers in general and hemp fibers in particular have great prospects for their use in various innovative applications such as ecological, biodegradable, and renewable resources with unique properties. Such properties together with the increased strength due to high-cellulose content and specific morphological parameters are widely used to produce plant fiber-based plastic composites. The properties of plant fibers that may influence the properties of composites depend on crop processing, but the basis for them is provided during fiber development in planta. It is known that two types of bast fibers are developed in the hemp stem: primary fibers formed from procambium cells and secondary fibers that originate as a result of cambium activity. Both types of fibers may significantly vary in their yield and quality depending on the variety and growth conditions. Differences in the anatomical and morphological characteristics of the two types of hemp fibers, together with peculiarities in the composition and architecture of cell wall, influence the technical parameters of the raw material quality. Based on our study of both primary and secondary fiber development in hemp stem that was focused on the two key stages, intrusive elongation and deposition of thick cell wall layers, we suggest the set of parameters that can influence the quality of the mature fibers and trace their biological origin.

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“…2A). For example, the relative abundances of rhamnogalacturonan I (RGI) backbone epitopes recognized by the mAbs INRA-RUI and INRA-RU2 (Ralet et al, 2010) were reduced by 58% and 68%, respectively, in CDTA fractions, while the relative level of binding of all HG-directed mAbs was reduced by between 32% and 57% ( Fig. 2A).…”

Section: Carbohydrate Microarray Profiling Provides An Overview Of Cementioning

confidence: 99%

Pea Border Cell Maturation and Release Involve Complex Cell Wall Structural Dynamics

et al. 2017

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The adhesion of plant cells is vital for support and protection of the plant body and is maintained by a variety of molecular associations between cell wall components. In some specialized cases, though, plant cells are programmed to detach, and root cap-derived border cells are examples of this. Border cells (in some species known as border-like cells) provide an expendable barrier between roots and the environment. Their maturation and release is an important but poorly characterized cell separation event. To gain a deeper insight into the complex cellular dynamics underlying this process, we undertook a systematic, detailed analysis of pea (Pisum sativum) root tip cell walls. Our study included immunocarbohydrate microarray profiling, monosaccharide composition determination, Fourier-transformed infrared microspectroscopy, quantitative reverse transcription-PCR of cell wall biosynthetic genes, analysis of hydrolytic activities, transmission electron microscopy, and immunolocalization of cell wall components. Using this integrated glycobiology approach, we identified multiple novel modes of cell wall structural and compositional rearrangement during root cap growth and the release of border cells. Our findings provide a new level of detail about border cell maturation and enable us to develop a model of the separation process. We propose that loss of adhesion by the dissolution of homogalacturonan in the middle lamellae is augmented by an active biophysical process of cell curvature driven by the polarized distribution of xyloglucan and extensin epitopes.

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Monoclonal antibodies to rhamnogalacturonan I backbone

Cited by 117 publications

References 45 publications

MUCILAGE-RELATED10 Produces Galactoglucomannan That Maintains Pectin and Cellulose Architecture in Arabidopsis Seed Mucilage

MUCILAGE-RELATED10 Produces Galactoglucomannan That Maintains Pectin and Cellulose Architecture in Arabidopsis Seed Mucilage

Development of Hemp Fibers: The Key Components of Hemp Plastic Composites

Pea Border Cell Maturation and Release Involve Complex Cell Wall Structural Dynamics

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