Monoclonal antibodies to soluble, human milk galactosyltransferase (lactose synthase a protein)

Berger, Eric G.; Aegerter, E.; Mandel, Theres; Hauri, Hans‐Peter

doi:10.1016/s0008-6215(00)90366-5

Cited by 37 publications

(27 citation statements)

References 27 publications

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“…p33/NAGT I was tagged with a myc epitope at the C-terminus (Figure 1), so was immunoprecipitated using the mAb 9E10 (Evan et al, 1985). Both p33/Mann II and p33/GalT were immunoprecipitated using polyclonal antibodies specific for the catalytic (lumenal) domain (Berger et al, 1986;. The immunoprecipitates were fractionated by SDS-PAGE.…”

Section: Resultsmentioning

confidence: 99%

Kin recognition between medial Golgi enzymes in HeLa cells.

et al. 1994

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Section: Resultsmentioning

confidence: 99%

Kin recognition between medial Golgi enzymes in HeLa cells.

et al. 1994

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“…Anti-GM130, anti-Syntaxin 6, and anti-␦-adaptin were obtained from BD Biosciences Transduction Laboratories (Lexington, KY). The mouse mAb against ␤-1,4-galactosyltransferase (GT) was from J. Rohrer (Institute of Physiology, University of Zü rich, Zü rich, Switzerland) (Berger et al, 1986). mAb against CD63 (H5C6) was obtained from the Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, IA).…”

Section: Reagentsmentioning

confidence: 99%

Clathrin-dependent Association of CVAK104 with Endosomes and theTrans-Golgi Network

Düwel

Ungewickell

2006

MBoC

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“…Antibody dilutions were as follows: Al /118 at 1:100, anti-giantin (Linstedt et al, 1995) at 1:1000, anti-galactosyltransferase (Berger et al, 1986) at 1:200, and anti-mannose-6-phosphate receptor at 1:500. Fixable fluorescein isothiocyanate (FITC)-dextran (Mr, 10,000, Molecular Probes, Eugene, OR) was added to DMEM at 1 mg/ml and the cells were cultured for 1 h and then fixed for 12 h. Antibody uptake was performed by incubation of cells with 1 ,ug/ml A1/118 or antigiantin for 3 h in DMEM.…”

Section: Immunofluorescence and Immunoelectron Microscopymentioning

confidence: 99%

Sequence and overexpression of GPP130/GIMPc: evidence for saturable pH-sensitive targeting of a type II early Golgi membrane protein.

Linstedt

Mehta

Suhan

et al. 1997

MBoC

113

165

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It is thought that residents of the Golgi stack are localized by a retention mechanism that prevents their forward progress. Nevertheless, some early Golgi proteins acquire late Golgi modifications. Herein, we describe GPP130 (Golgi phosphoprotein of 130 kDa), a 130-kDa phosphorylated and glycosylated integral membrane protein localized to the cis/medial Golgi. GPP130 appears to be the human counterpart of rat Golgi integral membrane protein, cis (GIMPc), a previously identified early Golgi antigen that acquires late Golgi carbohydrate modifications. The sequence of cDNAs encoding GPP130 indicate that it is a type II membrane protein with a predicted molecular weight of 81,880 and an unusually acidic lumenal domain. On the basis of the alignment with several rod-shaped proteins and the presence of multiple predicted coiled-coil regions, GPP130 may form a flexible rod in the Golgi lumen. In contrast to the behavior of previously studied type II Golgi proteins, overexpression of GPP130 led to a pronounced accumulation in endocytotic vesicles, and endogenous GPP130 reversibly redistributed to endocytotic vesicles after chloroquine treatment. Thus, localization of GPP130 to the early Golgi involves steps that are saturable and sensitive to lumenal pH, and GPP130 contains targeting information that specifies its return to the Golgi after chloroquine washout. Given that GIMPc acquires late Golgi modifications in untreated cells, it seems likely that GPP130/GIMPc continuously cycles between the early Golgi and distal compartments and that an unidentified retrieval mechanism is important for its targeting.

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Monoclonal antibodies to soluble, human milk galactosyltransferase (lactose synthase a protein)

Cited by 37 publications

References 27 publications

Kin recognition between medial Golgi enzymes in HeLa cells.

Kin recognition between medial Golgi enzymes in HeLa cells.

Clathrin-dependent Association of CVAK104 with Endosomes and theTrans-Golgi Network

Sequence and overexpression of GPP130/GIMPc: evidence for saturable pH-sensitive targeting of a type II early Golgi membrane protein.

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