Monoclonal antibody 18B8 detects gangliosides associated with neuronal differentiation and synapse formation.

Grunwald, Gerald B.; Fredman, Pam; Magnani, John L.; Trisler, David; Ginsburg, Victor; Nirenberg, Marshall W.

doi:10.1073/pnas.82.12.4008

Cited by 36 publications

(19 citation statements)

References 35 publications

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“…rule out the possibility, however, that other glycoconjugates might be transiently expressed by subpopulations of sensory neurons at earlier stages, as occurs elsewhere in the developing nervous system (Levine et al, 1984;Grunwald et al, 1985;Yamamoto et al, 1985;Dodd and Jessell, 1986;Schwarting et al, 1987). The possibility also exists that other populations of DRG neurons express carbohydrates with unique subterminal structures, which we would not have detected in the present lectin-binding studies (cf.…”

Section: Discussionmentioning

confidence: 52%

“…Cell surface glycoconjugates are likely to serve important functions in neural development, such as cell-cell recognition and adhesion (Edelman, 1984;Fischer and Csillik, 1985;Streit et al, 1985;Dodd and Jessell, 1986;Peinado et al, 1987), axon guidance (Dodd and Jessell, 1986), and synapse formation and/ or maintenance (Fischer and Csillik, 1985;Grunwald et al, 1985). Few of these functions have been directly demonstrated in viva (Fraser et al, 1988;Landmesser et al, 1988).…”

Section: Lectin Binding Identifies a Subpopulation Of Neurons In Chicmentioning

confidence: 99%

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Lectin binding identifies a subpopulation of neurons in chick dorsal root ganglia

1990

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We screened a variety of lectins with different sugar specificates to determine whether subpopulations of dorsal root ganglion (DRG) neurons in the chick can be distinguished by the carbohydrates they express. Of the 15 lectins tested only those that recognize N-acetylgalactosamine (galNac) residues labeled a subset of DRG neurons. For example, Dolichos biflorus (DBA) labeled a population of small-diameter neurons in the dorsomedial DRG and their terminals in the dorsal horn in hatchling chicks. Staining of live neurons in vitro demonstrated that DBA was binding to the cell surface. Labeling first appeared in sensory neurons at about St.38 (E12) and in dorsal horn laminae 1 and 2 at about St.42 (E16). Fainter labeling appeared somewhat later in lamina 3, after hatching. Labeling of the tissue sections was eliminated by chloroform: methanol extraction and reduced by alpha-N- acetylgalactosaminidase digestion, but survived trypsinization. Together these results suggest that a subset of DRG neurons in the chick can be identified by the presence of a cell surface glycoconjugate, perhaps a glycolipid, containing terminal alpha-linked galNac residues.

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Section: Discussionmentioning

confidence: 52%

Section: Lectin Binding Identifies a Subpopulation Of Neurons In Chicmentioning

confidence: 99%

Lectin binding identifies a subpopulation of neurons in chick dorsal root ganglia

1990

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“…A number of these, including antigen 7A and SSEA-1, that contain the X-determinant trisaccharide (Lagenaur et al, 1983;Yamamoto et al, 1985), the antigen recognized by anti-ganglioside antibody Q2 11 (Henke-Fahle, 1983;Rohrer et al, 1985;Rosner et al, 1985), and the groups of gangliosides recognized by antibody 18B8 (Grunwald et al, 1985;Dubois et al, 1986) show discrete patterns of labeling in the developing nervous system. Thus, there is increasing evidence that lipids bearing carbohydrate epitopes, as defined by monoclonal antibodies, can be distributed in very discrete spatial and temporal patterns in the developing nervous system.…”

Section: Potential Functional Role Of the Jones Gangliosidesmentioning

confidence: 99%

“…Among the glycolipids, gangliosides have attracted particular attention in the context of neurogenesis (Hakamori,198 1). These small sialic acid-bearing molecules have specific patterns of expression in different brain regions and are under rigid developmental control (Dreyfus et al, 1976;Rosner, 1980b, Willinger andSchachner, 1980;HenkeFahle, 1983;Grunwald et al, 1985;Yamamoto et al, 1985). They have also been implicated in a variety of developmental functions.…”

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confidence: 99%

Developmental regulation of ganglioside antigens recognized by the JONES antibody

et al. 1988

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The JONES monoclonal antibody has been immunocytochemically associated with regions of the developing rat brain where cell and axon migrations are occurring (Mendez-Otero et al., 1986, 1988). In the present study the antigens recognized by the JONES antibody were analyzed in a variety of brain regions and at developmental ages selected to correspond to the preceding immunocytochemical observations. In accordance with earlier results from retina, JONES binding could not be detected in SDS gels from developing brain. Binding of the antibody was, however, prominent in chloroform/methanol extracts of the same tissues, and it was completely removed from tissue sections by brief chloroform/methanol form/methanol treatment. Enzymatic analyses of chloroform/methanol extracts indicated that the JONES epitope was sensitive to neuraminidase but insensitive to proteases. Overlay assays on developed high-performance thin-layer chromatographic plates (HPTLC) indicate that in all regions the JONES epitope resides on 2 or 3 ganglioside bands, depending on the age examined. These bands migrate between ganglioside standards GD 1a and GM2 on HPTLC plates and have been designated GJ1, GJ2, and GJ3, with the higher number designating the more rapidly migrating species. Occasionally, additional bands migrating in the range of polysialogangliosides were observed. The pattern of expression of GJ species was studied in forebrain, retina, and cerebellar tissue taken from embryonic day 18 (E18), postnatal day 0 (P0), P7, P14, and adult animals. Both region-specific differences in the relative prominence of each band and stage-specific differences in the total amount of the JONES gangliosides were detected. The stage- specific differences in the amount of JONES antigens are well correlated with the developmental periods of maximal cell migration in each region. While the JONES gangliosides are most prominent in forebrain before birth, in they are most prominent during the first 2 postnatal weeks. In cerebellum, JONES antigen expression is more pronounced during the 2 periods of cell migration in this tissue. In retina, the more rapidly migrating GJ3 band was the most prominent band at all stages examined, and this same band is retained in the adult. In cerebellum and forebrain GJ3 is also the most pronounced band during development. However, in contrast to the retina, the more slowly migrating GJ1 band is retained in adult forebrain and cerebellum. A variety of non-brain tissues have also been examined for the presence of the JONES antigens.

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“…For instance, the ganglioside GQlb enhances greatly neurite outgrowth and cell proliferation in neuroblastoma cell lines (Isuji et al, 1983). Gangliosides detected by a monoclonal antibody were shown to be developmentally regulated in chicken retina and brain (Grunwald et al, 1985) and a phosphorylated ganglioside was reported to stimulate species-specific adhesion of chicken hepatocytes (Roseman, 1985).…”

Section: Discussionmentioning

confidence: 99%

A novel glycosphingolipid that may participate in embryo inversion in volvox carteri

Wenzl

Sumper

1986

Cell

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Monoclonal antibody 18B8 detects gangliosides associated with neuronal differentiation and synapse formation.

Cited by 36 publications

References 35 publications

Lectin binding identifies a subpopulation of neurons in chick dorsal root ganglia

Lectin binding identifies a subpopulation of neurons in chick dorsal root ganglia

Developmental regulation of ganglioside antigens recognized by the JONES antibody

A novel glycosphingolipid that may participate in embryo inversion in volvox carteri

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