Monoclonal Antibody 2–2-B Kills K1-positive Escherichia coli in Conjunction with Cord Blood Neutrophils and Sera, but Not with Spinal Fluid

ABSTRACT'. Patients with cystic fibrosis (CF) whose respiratory tracts are colonized with Pseudomonas aeruginosa (PA) may develop a specific opsonic deficiency for alveolar macrophage phagocytosis of PA. We examined the possible role of altered antibody (Ab) isotype in this phenomenom by measuring serum levels and distribution of IgG and IgG subclass Ab (IgGl, IgG2, IgG3, and IgG4) to the major opsonic immunodeterminant, serotype-specific lipopolysaccharide (LPS), by means of enzyme-linked immunosorbent assays employing monoclonal secondary antibodies, and comparing these results to the serum opsonic capacity in an in vitro murine alveolar macrophage phagocytic assay. Twenty-one patients with CF who were colonized with PA had approximately a 30-fold elevation of PA LPS IgG Ab levels and higher IgG subclass 1-4 Ab compared to 10 uncolonized patients with CF and 11 healthy controls (p < 0.05-0.0005 depending on the isotype). Colonized patients with CF had a shift in PA LPS Ab distribution toward IgG3 compared to uncolonized patients with CF (p < 0.02). A surprising finding was that uncolonized patients with CF had lower levels (p < 0.05) and proportion (p < 0.002) of PA LPS IgG2 Ab than controls, with an apparent shift to higher levels and proportion of PA LPS IgG4 (p < 0.01). Serum from colonized patients with CF showed diminished opsonic capacity for phagocytosis of PA compared to uncolonized patients and controls (p < 0.005), with 42% showing inhibitory activity.

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Monoclonal Antibody 2–2-B Kills K1-positive Escherichia coli in Conjunction with Cord Blood Neutrophils and Sera, but Not with Spinal Fluid

Abstract: SummaryIn conjunction with 400 ngfml murine monoclonal antibody of . the IgM class, cord neutrophils were able to kill KI-positive

Cited by 4 publications

References 5 publications

Immunomodulating Agents

Immunomodulating Agents

Monoclonal antibodies

Altered Antibody Isotype in Cystic Fibrosis. Possible Role in Opsonic Deficiency

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