Monoclonal Antibody Against a Melanosomal Protein in Melanotic and Amelanotic Human Melanoma Cells

McEwan, Max; Parsons, Peter G.; Moss, Denis J.; Burrows, Scott R.; Stenzel, Deborah; Bishop, Chris; Strutton, Geoffrey

doi:10.1111/j.1600-0749.1989.tb00150.x

Cited by 11 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Color was developed by reaction with 50 pl of 7.5 mM BCIPl0.7 mM NBT at room temperature for 30 min. Positive staining with both the rabbit anti-bovine SlOO antibody (DAKOPA'M'S; Nakajima et al, 1982) and the monoclonal antibody l C l l to a melanosomal antigen (McEwan et al, 1989) confirmed the identity of cells as melanoma.…”

Section: Immunohistochemistrymentioning

confidence: 81%

In Vivo and In Vitro Expression of Octamer Binding Proteins in Human Melanoma Metastases, Brain Tissue, and Fibroblasts

Thomson

Parsons

Sturm

1993

Pigment Cell Research

Self Cite

View full text Add to dashboard Cite

The pattern of octamer sequence-specific DNA binding proteins expressed in human melanoma was examined in nuclear extracts of seven surgically-isolated tumors, short-term cultures of these tumors, and 25 human melanoma cell lines to determine the in vivo and in vitro distribution of the melanocytic-associated Oct-M1 and Oct-M2 octamer binding activities. In the biopsy tissue and cultured melanoma cells of a metastasis from the cerebellum, two other binding activities (N-Oct-2 and N-Oct-6) in addition to the Oct-M1, Oct-M2 and the generally expressed Oct-1 protein were detected; this profile was consistent with that seen in normal human and mouse brain tissue. Melanoma tissue removed from lymph nodes and cell lines established from them also showed Oct-1, Oct-M1, Oct-M2, and N-Oct-2. N-Oct-2 was distinguished from the comigrating Oct-2A activity by failure to react with Oct-2A-specific antibody. All but one of the 25 melanoma cell lines exhibited Oct-1, Oct-M1, and Oct-M2 and/or N-Oct-2 activity, whereas cultured normal melanocytes expressed only Oct-1 and Oct-M1. In contrast to murine fibroblasts, which express only Oct-1, human fibroblast strains also expressed Oct-2A binding activity, which was confirmed by reactivity with Oct-2A antibody and the presence of Oct-2A mRNA and indicated that Oct-2A has a more general role than that of a lymphoid-specific transcription factor. Overall, the results indicate that expression of neural-specific Oct factors in human melanoma is (1) aberrant compared with normal melanocytes, (2) can be modulated by the surrounding tissue in a brain metastasis, and (3) may be part of the altered program of differentiation accompanying transformation.

show abstract

Section: Immunohistochemistrymentioning

confidence: 81%

In Vivo and In Vitro Expression of Octamer Binding Proteins in Human Melanoma Metastases, Brain Tissue, and Fibroblasts

Thomson

Parsons

Sturm

1993

Pigment Cell Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several studies have reported the development of murine MoAbs against human melanosomes by immunising animals with intact human melanoma cells or with melanosomes derived from these cells (McEwan et al, 1989;Hayashibe et al, 1986). Four MoAbs, HMSA-1 to 4, previously developed by the latter method in this laboratory (Akutsu & Jimbow, 1986;Jimbow et al, 1990) were shown to recognise melanosomal antigens highly specific to neoplastic melanocytes.…”

Section: Discussionmentioning

confidence: 99%

A murine monoclonal antibody, MoAb HMSA-5, against a melanosomal component highly expressed in early stages, and common to normal and neoplastic melanocytes

Der

Dixon²,

Jimbow³

et al. 1993

Br J Cancer

View full text Add to dashboard Cite

Summary The melanosome is a secretory organelle unique to the melanocyte and its neoplastic counterpart, malignant melanoma. The synthesis and assembly of these intracytoplasmic organelles is not yet fully understood. We have developed a murine monoclonal antibody (MoAb) against melanosomes isolated from human melanocytes (newborn foreskin) cultured in the presence of 12-0 tetradecanoyl phorbol-13-acetate (TPA). This MoAb, designated HMSA-5 (Human Melanosome-Specific Antigen-5) (IgG1), recognised a cytoplasmic antigen in both normal human melanocytes and neoplastic cells, such as common and dysplastic melanocytic nevi, and malignant melanoma. None of the carcinoma or sarcoma specimens tested showed positive reactivity with MoAb HMSA-5. Under immunoelectron microscopy, immuno-gold deposition was seen on microvesicles associated with melanosomes, and a portion of the ER-Golgi complexes. Radioimmunoprecipitation analysis showed that the HMSA-5 reactive antigen was a glycoprotein of M, 69 to 73 kDa. A pulse-chase time course study showed that the amount of antigen detected by MoAb HMSA-5 decreased over a 24 h period without significant expression on the cell surface, or corresponding appearance of the antigen in the culture supernatant. This glycoprotein appears to play a role in the early stages of melanosomal development, and the HMSA-5 reactive epitope may be lost during subsequent maturation processes. Importantly, HMSA-5 can be identified in all forms of human melanocytes, hence it can be considered a new common melanocytic marker even on routine paraffin sections.

show abstract

“…MoAbs l C l l and 5C12 react with human melanosomal antigens and have been described previously (McEwan et al, 1988(McEwan et al, ,1989. MoAb 2B7 binds active tyrosinase but appears to recognise a different epitope from MoAb 5C12 (unpublished).…”

Section: Enzyme Assaysmentioning

confidence: 99%

Histopathology of Melanocytic Lesions in Goats and Establishment of a Melanoma Cell Line: A Potential Model for Human Melanoma

Parsons

Takahashi

Candy

et al. 1990

Pigment Cell Research

View full text Add to dashboard Cite

Melanocytic cells from white Angora goats were studied in vivo and in vitro. The histopathology of pigmented areas of skin from the most common sites of melanoma (solar-exposed areas of the ear, face, and perineum) resembled that of the epidermal melanocytes in Hutchinson's melanotic freckle in humans. Seven melanoma biopsies from 6 Angora goats showed histopathological features in common with human melanoma. A melanoma cell line, GM-1, was established in culture from a lymph node metastasis obtained from an animal that had a primary tumor excised and later developed extensive metastatic disease. GM-1 cells were mainly diploid, amelanotic, proliferated rapidly, spontaneously formed vacuolated cells, and were tumorigenic in nude mice. The species of origin of the GM-1 line was confirmed by isozyme profiles. GM-1 cultured cells and the original biopsy both expressed S-100 protein and tyrosinase antigen. Using GM-1 cells as the immunogen, a monoclonal antibody (MoAb 1F1) was derived that reacted strongly with a 116 kDa antigen in 50% of the GM-1 cells, but had little activity with goat fibroblasts (GM-F) or with human melanoma cells. GM-F, on the other hand, yielded more intense staining than GM-1 with an intermediate filament antibody (IFA), reacting with a 58 kDa antigen in both cell lines. The sensitivity of GM-1 to anticancer agents was similar to that of human melanoma cells. The pathology of caprine melanoma and its association with sun-exposed sites in relatively young animals suggest that it may be a suitable model for studying induction of melanoma by natural sunlight.

show abstract

Monoclonal Antibody Against a Melanosomal Protein in Melanotic and Amelanotic Human Melanoma Cells

Cited by 11 publications

References 19 publications

In Vivo and In Vitro Expression of Octamer Binding Proteins in Human Melanoma Metastases, Brain Tissue, and Fibroblasts

In Vivo and In Vitro Expression of Octamer Binding Proteins in Human Melanoma Metastases, Brain Tissue, and Fibroblasts

A murine monoclonal antibody, MoAb HMSA-5, against a melanosomal component highly expressed in early stages, and common to normal and neoplastic melanocytes

Histopathology of Melanocytic Lesions in Goats and Establishment of a Melanoma Cell Line: A Potential Model for Human Melanoma

Contact Info

Product

Resources

About