Monoclonal antibody against a “Pan-T-Cell” antigen expressed by thymocytes, peripheral T-lymphocytes and T-cell leukemias

Carrel, Stefan; Groß, Nicole; Heumann, Didier; Sékaly, Rafick Pierre; Girardet, Christophe; Schmidt-Kessen, Andreas; Mach, Jean‐Pierre

doi:10.1016/0161-5890(84)90136-6

Cited by 23 publications

(10 citation statements)

References 26 publications

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“…Injection of mRNA into Xenopus oocytes, treatment of the oocytes with tunicamycin, immunoprecipitation of translation products with anti-DR monoclonal antibodies, and analysis by gel electrophoresis have been described (9). When indicated, samples were prepared for electrophoresis under nonreducing condi- (20). Peptide Maps.…”

Section: Methodsmentioning

confidence: 99%

Isolation of cDNA clones for the p33 invariant chain associated with HLA-DR antigens.

Long¹,

Strubin²,

Wake³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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HLA-DR antigens are polymorphic cell surface glycoproteins involved in the control of the immune response in man. They consist of two subunits, the cr and the ,B chains. In addition, an invariant glycoprotein of Mr 33,000 (DRp33) is associated intracellularly with HLA-DR antigens. A cDNA clone for DRp33, called 33-10, was isolated. Because no amino acid sequence has yet been determined for DRp33 the identification of cDNA clone 33-10 was based on selection of mRNA by hybridization, subsequent translation in a rabbit reticulocyte lysate supplemented with microsomes, and translation in microinjected Xenopuw oocytes followed by immunoprecipitation with an anti-DR antiserum. The translation products assembled with DR a and (3 chains in oocytes coinjected with all three mRNAs. Assembly of DR a and .3 chains was also observed in the absence of DRp33 mRNA. Furthermore, when compared with DRp33 immunoprecipitated from a human B-cell line, translation products of the hybrid-selected mRNA showed (i) identical migration in two-dimensional gel electrophoresis, (ii) identical apparent molecular weight in the absence of N-linked glycosylation, and (iii) a very similar two-dimensional peptide map. Transcription of the DRp33 gene into a mRNA 1,400 nucleotides long was observed in B cells but was undetectable in T-cell lines and was very low in liver. Thus, DRp33 appears to be coordinately expressed with DR a and (8 chains. Hybridization to DNA of mouse-human somatic cell hybrids showed that DRp33 is encoded by a gene that is located outside the major histocompatibility complex.

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Section: Methodsmentioning

confidence: 99%

Isolation of cDNA clones for the p33 invariant chain associated with HLA-DR antigens.

Long¹,

Strubin²,

Wake³

et al. 1983

Proc. Natl. Acad. Sci. U.S.A.

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“…On the other hand, CALLA is found on lymphoblastic leukemias, on certain lymphomas, and on melanomas and glioma cell lines (1,5,7,8,32 Cell surface-labeling and immunoprecipitation. Cells from the NALM-I or Mel-1477 lines (1 X 107) were labeled with Na'251 using the lactoperoxidase/glucose oxidase-catalyzed reaction, and surface antigens were immunoprecipitated from a 1% Genapol X-080 (Calbiochem, Lucerne, Switzerland) extract as described earlier (38).…”

Section: Introductionmentioning

confidence: 99%

“…The common acute lymphoblastic leukemia antigen (CALLA; CD10)' is a membrane glycoprotein of Mr 95,000-100,000 (1)(2)(3)(4)(5)(6)) that was first identified on lymphoblasts of patients with the common (pre-B) type of acute lymphoblastic leukemia (ALL) (1,2,7,8). While CALLA is not expressed on normal T 1.…”

Section: Introductionmentioning

confidence: 99%

Common acute lymphoblastic leukemia antigen expressed on leukemia and melanoma cell lines has neutral endopeptidase activity.

Jongeneel

Quackenbush²,

Ronco³

et al. 1989

J. Clin. Invest.

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We have previously reported that the amino acid sequence of the common acute lymphoblastic leukemia antigen (CALLA, CD10) translated from a normal human kidney cDNA clone is identical to that of neutral endopeptidase (NEP, EC 3.4.24.11). In this study, we show that by flow cytometry, a monoclonal antibody (135A3) produced against rabbit NEP reacted selectively with leukemia and melanoma cell lines expressing CALLA on their surface. A glycoprotein of apparent

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“…LAU-A1, as 3A1 described by Haynes et al [5], reacts with a membrane glycoprotein of approxi mately 40,000 daltons, which is expressed on normal thymocytes and peripheral T cells but also on malig nant T cells [6]. If compared to Leu-1, LAU-A1 reacted with a larger spectrum of pediatric T cell neo plasia (table II), stained a higher percentage of ma lignant cells and produced a brighter fluorescence.…”

Section: Discussionmentioning

confidence: 99%

Reactivity of Monoclonal Antibodies LAU-A1 and Anti-Y 29/55 in T and B Cell Malignancies of Children: Correlation with Immunological Markers and Clinical Data

Hirt

Carrel²,

Forster

et al. 1986

Acta Haematol

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The monoclonal anti-pan-T cell antibody LAU-A1 stained neoplastic T cells arrested at different levels of maturation from all 21 children with T cell malignancies examined. Particularly in 7 patients with immature T cell neoplasia staining for LAU-A1 facilitated the recognition of a T cell origin of the malignant cells. Only 40% of these immature T cell malignancies were associated with an anterior mediastinal mass. A subdivision of T cell neoplasia into 4 differentiation-related subgroups did not permit to make predictions regarding the patients’ survival. Despite the rather uniform clinical presentation the immunological phenotypes of tumor cells in 14 children with B cell non-Hodgkin’s lymphoma (B NHL) were heterogeneous. Tumor cells lacked surface immunoglobulins (2 patients), expressed IgM only (7 patients), IgM and IgD (3 patients) or IgM, IgD and IgA (2 patients). Regardless of surface immunoglobulin expression anti-Y 29/55 stained practically all recognizable tumor cells of all B NHL examined. No correlation was found between the number of heavy-chain isotypes expressed on tumor cells and the survival of the patients. The only long-term survivors were 3 children transplanted with autologous bone marrow which had been purged in vitro with anti-Y 29/55 and complement.

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Monoclonal antibody against a “Pan-T-Cell” antigen expressed by thymocytes, peripheral T-lymphocytes and T-cell leukemias

Cited by 23 publications

References 26 publications

Isolation of cDNA clones for the p33 invariant chain associated with HLA-DR antigens.

Isolation of cDNA clones for the p33 invariant chain associated with HLA-DR antigens.

Common acute lymphoblastic leukemia antigen expressed on leukemia and melanoma cell lines has neutral endopeptidase activity.

Reactivity of Monoclonal Antibodies LAU-A1 and Anti-Y 29/55 in T and B Cell Malignancies of Children: Correlation with Immunological Markers and Clinical Data

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