Monoclonal antibody against Fasciola gigantica glutathione peroxidase and their immunodiagnosis potential for fasciolosis

Kueakhai, Pornanan; Chaithirayanon, Kulathida; Chaiwichien, Athit; Samrit, Tepparit; Osotprasit, Supawadee; Suksomboon, Phawiya; Jaikua, Wipaphorn; Sobhon, Prasert; Changklungmoa, Narin

doi:10.1016/j.vetpar.2019.108979

Cited by 6 publications

(2 citation statements)

References 27 publications

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“…Since this study did not obtain sheep serum from days 4 to 12 of infection, whether our method can detect circulating antigens during this period needs further experimental verification. Similarly, Pornanan Kueakhai used the anti-rFgGPx monoclonal antibody to detect circulating antigens in mouse serum 1 week after infection with F. gigantica, but he did not conduct artificial infection experiments with F. gigantica in large animals such as cattle and sheep [41]. We found that there are almost no reported articles on the detection of circulating antigens in sheep infected with F. hepatica, and there is also no commercialized serum circulating antigen detection kit.…”

Section: Discussionmentioning

confidence: 95%

The Detection of Circulating Antigen Glutathione S-Transferase in Sheep Infected with Fasciola hepatica with Double-Antibody Sandwich Signal Amplification Enzyme-Linked Immunosorbent Assay

Duan,

Zhang,

Liu

et al. 2024

Animals

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Fasciolosis is a global zoonotic parasitic disease caused by F. hepatica infection that is particularly harmful to cattle and sheep. A biotin–streptavidin signal amplification ELISA (streptavidin-ELISA/SA-ELISA) based on circulating antigens can allow for the early detection of F. hepatica-infected animals and is suitable for batch detection. It is considered to be a better means of detecting F. hepatica infection than traditional detection methods. In this study, using the serum of sheep artificially infected with F. hepatica, the cDNA expression library of F. hepatica was screened, 17 immunodominant antigen genes of F. hepatica were obtained, and glutathione s-transferase (GST) was selected as the candidate detection antigen. Firstly, the GST cDNA sequence was amplified from F. hepatica, followed by the preparation of recombinant protein GST (rFhGST). Then, monoclonal and polyclonal antibodies against rFhGST were prepared using the GST protein. Afterward, the immunolocalization of the target protein in the worm was observed via confocal microscopy, and it was found that the GST protein was localized in the uterus, intestinal tract, and body surface of F. hepatica. Finally, a double-antibody sandwich SA-ELISA based on the detection of circulating antigens was established. There was no cross-reaction with positive sera infected with Dicrocoelium lanceatum (D. lanceatum), Haemonchus contortus (H. contortus), Neospora caninum (N. caninum), or Schistosoma japonicum (S. japonicum). Forty serum and fecal samples from the same batch of sheep in Nong’an County, Changchun City, Jilin Province, China were analyzed using the established detection method and fecal detection method. The positive rate of the SA-ELISA was 17.5%, and the positive rate of the fecal detection method was 15%. The detection results of this method were 100% consistent with commercial ELISA kits. A total of 152 sheep serum samples were tested in Nong’an County, Changchun City, Jilin Province, and the positive rate was 5.92%. This study laid the foundation for the development of serological detection preparations for F. hepatica infection based on the detection of circulating antigens.

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Section: Discussionmentioning

confidence: 95%

The Detection of Circulating Antigen Glutathione S-Transferase in Sheep Infected with Fasciola hepatica with Double-Antibody Sandwich Signal Amplification Enzyme-Linked Immunosorbent Assay

Duan,

Zhang,

Liu

et al. 2024

Animals

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“…Моноклональные антитела (MoAb) против рекомбинантной глутатионпероксидазы F. gigantica (rFgGPx) могут быть использованы для иммунодиагностики как раннего, так и позднего фасциолеза у животных и людей [54,55], а рекомбинантную аденилаткиназу 3 предлагают в качестве маркера серодиагностики клонорхоза [56]. Определение уровня катепсина в случае инвазии O. viverrini также по-казывает хорошие результаты -чувствительность и специфичность 62,1 и 84,1% соответственно, поэтому он может стать иммунодиагностической альтернативой при описторхозе человека в эндемичных районах [57,58].…”

Section: технологии иммуноферментного анализаunclassified

Foresight in the diagnosis of trematodiasis: innovations versus routine methods

et al. 2023

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Aim. To analyze modern methods for the diagnosis of trematodiasis in experimental and epidemiological studies.Trematodiasis is a group of common parasitic diseases that are a socially sensitive health problem worldwide. According to World Health Organization, more than 250 million people are affected by trematode infections globally. The most common types of human trematode infections are diseases caused by Schistosoma, Fasciola, Clonorchis, and Opisthorchis pathogens. Diagnosis of trematodiasis is often multistage and includes identification of disease symptoms, analysis of medical history, and use of various laboratory tests. Clinical presentation of parasitic infections often varies, making a definitive diagnosis difficult. Various tools are used to diagnose trematode infections: epidemiological criteria, laboratory tests (complete blood count and blood biochemistry, serological methods), instrumental methods (abdominal X-ray and ultrasound), and parasitological techniques, which often have insufficient sensitivity and specificity. Therefore, development of modern and effective non-invasive methods for detection of trematode infections with high sensitivity and specificity, including screening in endemic regions, is relevant.The present review analyzes the results of 90 clinical trials and experimental studies on the diagnosis of trematode infections using the PubMed search engine and the eLibrary database. The review analyzes original articles published from January 1, 2015 to December 31, 2021.Most studies confirm that the absence of a standard diagnostic approach highlights obvious convenience of utilizing a combined approach to reliable diagnosis of trematodiasis. An adequate combination of different diagnostic tests makes it possible to diagnose the disease correctly, devise a correct treatment and follow-up strategy, and organize preventive measures.

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Livestock health: current status of helminth infections and their control for sustainable development

Abidi

2022

Advances in Animal Experimentation and Modeling

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Monoclonal antibody against Fasciola gigantica glutathione peroxidase and their immunodiagnosis potential for fasciolosis

Cited by 6 publications

References 27 publications

The Detection of Circulating Antigen Glutathione S-Transferase in Sheep Infected with Fasciola hepatica with Double-Antibody Sandwich Signal Amplification Enzyme-Linked Immunosorbent Assay

The Detection of Circulating Antigen Glutathione S-Transferase in Sheep Infected with Fasciola hepatica with Double-Antibody Sandwich Signal Amplification Enzyme-Linked Immunosorbent Assay

Foresight in the diagnosis of trematodiasis: innovations versus routine methods

Livestock health: current status of helminth infections and their control for sustainable development

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