Monoclonal Antibody against Mycoplasma fermentans‐Specific Aminoglycoglycerolipid

Abstract

Previously, we reported that Mycoplasma fermentans has specific antigens (phosphocholinecontaining glycoglycerolipids: GGPL-I and GGPL-III) and discussed the possibility of their pathogenic role. In this paper, we report the characterization of a monoclonal antibody (MF-III-1) specific to GGPL-III (phosphocholine-containing aminoglycoglycerolipid) using methods of electron microscopy, immunofluorescence cell surface staining, laser scanning microscopy, immunoelectron microscopy, and thin-layer chromatography immunostaining. The MF-III-1 antibody specifically recognized M. fermentans attached to the surface of HTLV-I-infected human helper T-cells, and it did not cross-react with other lipids nor with human T-cell antigens. Since MF-III-1 distinguishes GGPL-III from GGPL-I, the binding site may include a serinol (2-amino-1,3-propanediol) residue of GGPL-III. MF-III-1 is useful for the in vitro study of M. fermentans, and may also be useful as a tool for the study of the involvement of M. fermentans in human diseases.

Structural elucidation of novel phosphocholine-containing glycosylinositol-phosphoceramides in filamentous fungi and their induction of cell death of cultured rice cells

“…Furthermore, PC was found in lipoteichoic acid [47] and the cell wall associated with teichoic acid of Streptococcus pneumoniae [48], in the lipopolysaccharide of Haemophilus influenzae [49], and in the glycoglycerolipid of Mycoplasma fermentans [20]. PC-containing glycoglycerolipids of Mycoplasma fermentans were reported to be one important factor associated with rheumatoid arthritis [50]. A striking finding in the present work is the presence of PC-containing GSLs in fungi.…”

“…There have been no previous reports of the existence of glycolipid-conjugated PC in fungi, and this is the first study revealing the occurrence of GSLs that include both PC and inositol phosphate in organisms. PC-containing GSLs may also have important roles in the fungal infection of mammals, since PCcontaining molecules have been found in many pathogens [20,[47][48][49][50] and parasitic nematodes [21][22][23], and have been shown to modulate host-pathogen interactions [24].…”

An easy α-glycosylation methodology for the synthesis and stereochemistry of mycoplasma α-glycolipid antigens

“…In recent biochemical studies, Mycoplasma outer-membrane lipoproteins [2–3] and glycolipids [4–6] are thought to serve not only as the main antigens but also as probable pathogens. Also in our research team, Matsuda et al [7–10] isolated a new class of α-glycolipid antigens (GGPL-I and GGPL-III, Fig. 1) from M. fermentans .…”

Micoplasmas y su importancia médica.

“…Los ensayos inmunológicos con anti-GGPL-III permitieron detectar a este glicofosfolípido en individuos infectados con VIH. La frecuencia de anticuerpos contra GLP-III en pacientes infectados con VIH fue de 44.6 % (29/65), los donadores negativos al VIH presentaron 1.7 % (2/17), y los pacientes con otras enfermedades presentaron 0 % (0/20) y 8.7 % (2/23) (47,48).…”