Monoclonal antibody-based immunoassays for cyprodinil residue analysis in QuEChERS-based fruit extracts

Esteve‐Turrillas, Francesc A.; Abad‐Somovilla, Antonio; Quiñones-Reyes, Guillermo; Agulló, Consuelo; Mercader, Josep V.; Abad‐Fuentes, Antonio

doi:10.1016/j.foodchem.2015.04.119

Cited by 24 publications

(13 citation statements)

References 18 publications

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“…As with other mAbs, linker-site heterologous haptens have demonstrated to be an excellent approach in order to enhance assay sensitivity in indirect assays. [23][24][25] In summary, the antibodies obtained with hapten FXn showed superior affinity and specificity to fluxapyroxad than those derived from hapten FXb, thus evidencing the relevance of the linker position in the binding properties of the generated antibodies, as also demonstrated for other low molecular weight compounds. [26][27][28] For further cELISA development, two combinations of immunoreagents were selected according to the lowest IC 50 value and moderate slope of the inhibition curve, one for the direct format and the other for the indirect format.…”

Section: Hapten Preparation and Conjugationmentioning

confidence: 84%

Combined heterologies for monoclonal antibody-based immunoanalysis of fluxapyroxad

Ceballos-Alcantarilla

López-Puertollano

Agulló

et al. 2018

Analyst

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Section: Hapten Preparation and Conjugationmentioning

confidence: 84%

Combined heterologies for monoclonal antibody-based immunoanalysis of fluxapyroxad

Ceballos-Alcantarilla

López-Puertollano

Agulló

et al. 2018

Analyst

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“…This pretreatment method involves only three steps: extraction of sample with organic solvent, transfer of the extract to a d-SPE tube and cleanup by d-SPE. The d-SPE uses less sorbent and equipment while also saving time, effort and money, thus making it more applicable in the sample preparation procedure before analysis by immunoassay323334.…”

Section: Resultsmentioning

confidence: 99%

Development and comparative study of chemosynthesized antigen and mimotope-based immunoassays for class-specific analysis of O,O-dimethyl organophosphorus pesticides

Zhao

Wang

Han

et al. 2016

Sci Rep

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The multi-residue determination of organophosphorus pesticides (OPs) is an important task due to the wide application and high toxicity of OPs. However, there is no promising immunoassay to monitor the multi-residue of O,O-dimethyl OPs. In this study, a monoclonal antibody (mAb) against a generic hapten of O,O-dimethyl OPs (O,O-dimethyl O-(3-carboxyphenyl)phosphorothioate) was prepared. To develop an effective class-specific immunoassay, two strategies were performed to select the appropriate coating antigen or competing antigen. On the one hand, a total of 20 haptens were chemosynthesized, attached to ovalbumin for use as coating antigen candidates, and selected by direct competitive ELISA (dcELISA). As a second strategy, mimotopes of the mAb were selected from a random phage-display peptide library by panning, and the optimum mimotope was expressed as a fusion protein and biotinylated in vitro. Based on the selected chemosynthesized coating antigen and the biotinylated mimotope fusion protein, two sensitive broad-specificity dcELISAs were developed. The sensitivity, selectivity and practicability of the two immunoassays were compared. The results demonstrated that both methods showed similar selectivity and sensitivity and were reliable for O,O-dimethyl OP residues screening. However, the screening operation of mimotopes was much simpler and safer compared to the preparation of chemosynthesized coating antigens.

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“…Some ELISAs were developed for the detection of mepanipyrim and its related anilinopyrimidine fungicides. [16][17][18][19] They were sufficiently sensitive for mepanipyrim detection but their reactivity with the propanol type was not considered. This study re-ports that monoclonal antibodies (MoAbs) reacted with mepanipyrim and/or its propanol type were prepared by improving the hapten design and that the direct competitive (dc)-ELISAs based on their MoAbs could be applied to residue analysis in vegetables.…”

Section: Introductionmentioning

confidence: 99%

Development of a direct competitive enzyme-linked immunosorbent assay for determination of the fungicide mepanipyrim and its metabolite

et al. 2019

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A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was developed for determination of anilinopyrimidine fungicide mepanipyrim in vegetables. Two derivatives of mepanipyrim and mepanipyrim propanol type metabolite which carried carboxy acid were synthesized and conjugated with keyhole limpet hemocyanin. BALB/c mice were immunized to prepare anti-mepanipyrim monoclonal antibodies (MoAbs) by obtained conjugates. The dc-ELISAs based on the prepared MoAbs, MPP107 and MPP204, showed working ranges between 0.12 and 1.8 ng/mL with mepanipyrim for MPP107, 0.12 and 2.4 ng/mL with mepanipyrim for MPP204, and 0.2 ng/mL and 5.7 ng/mL with the mepanipyrim propanol type for MPP204. The dc-ELISAs showed the sufficient sensitivity to determine the mepanipyrim residues for the MRLs of 1-15 mg/kg among the majority of vegetables and fruits in Japan. Recovery and/or correlation results from HPLC suggested that the dc-ELISAs would be applicable to the residue analysis of mepanipyrim and its propanol type in vegetables.

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Monoclonal antibody-based immunoassays for cyprodinil residue analysis in QuEChERS-based fruit extracts

Cited by 24 publications

References 18 publications

Combined heterologies for monoclonal antibody-based immunoanalysis of fluxapyroxad

Combined heterologies for monoclonal antibody-based immunoanalysis of fluxapyroxad

Development and comparative study of chemosynthesized antigen and mimotope-based immunoassays for class-specific analysis of O,O-dimethyl organophosphorus pesticides

Development of a direct competitive enzyme-linked immunosorbent assay for determination of the fungicide mepanipyrim and its metabolite

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