Monoclonal antibody based inhibition ELISA as a new tool for the analysis of melamine in milk and pet food samples

Zhou, Yu; Li, Chunyuan; Li, Yansong; Ren, Hong-Lin; Lu, Shiying; Tian, Xiangli; Hao, Ya-Ming; Zhang, Yuanyuan; Shen, Qing-Feng; Liu, Zengshan; Meng, Xianmei; Zhang, Junhui

doi:10.1016/j.foodchem.2012.07.053

Cited by 51 publications

(14 citation statements)

References 27 publications

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“…Since 2007, a series of scandals involving the adulteration and contamination of dairy products with melamine and other compounds such as cyanuric acid, ammeline, and ammelide have brought serious challenges to Chinese food quality supervision departments [7–12]. To deal with the crisis, numerous targeted analysis methods were developed to detect melamine and its analogues in dairy products, including liquid chromatography [13, 14], gas chromatography [15], laser Raman spectrometry [16], immunoassay [17], electrochemical analysis [18], flow injection analysis [19], and infrared spectroscopy [20–22] among others [23–25]. However, new reports on adulterations with illegal ingredients other than melamine and its analogues are popping up from time to time.…”

Section: Introductionmentioning

confidence: 99%

The Feasibility of Using Near-Infrared Spectroscopy and Chemometrics for Untargeted Detection of Protein Adulteration in Yogurt: Removing Unwanted Variations in Pure Yogurt

Yan

Cai

et al. 2013

Journal of Analytical Methods in Chemistry

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Untargeted detection of protein adulteration in Chinese yogurt was performed using near-infrared (NIR) spectroscopy and chemometrics class modelling techniques. sixty yogurt samples were prepared with pure and fresh milk from local market, and 197 adulterated yogurt samples were prepared by blending the pure yogurt objects with different levels of edible gelatin, industrial gelatin, and soy protein powder, which have been frequently used for yogurt adulteration. A recently proposed one-class partial least squares (OCPLS) model was used to model the NIR spectra of pure yogurt objects and analyze those of future objects. To improve the raw spectra, orthogonal projection (OP) of raw spectra onto the spectrum of pure water and standard normal variate (SNV) transformation were used to remove unwanted spectral variations. The best model was obtained with OP preprocessing with sensitivity of 0.900 and specificity of 0.949. Moreover, adulterations of yogurt with 1% (w/w) edible gelatin, 2% (w/w) industrial gelatin, and 2% (w/w) soy protein powder can be safely detected by the proposed method. This study demonstrates the potential of combining NIR spectroscopy and OCPLS as an untargeted detection tool for protein adulteration in yogurt.

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Section: Introductionmentioning

confidence: 99%

The Feasibility of Using Near-Infrared Spectroscopy and Chemometrics for Untargeted Detection of Protein Adulteration in Yogurt: Removing Unwanted Variations in Pure Yogurt

Yan

Cai

et al. 2013

Journal of Analytical Methods in Chemistry

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“…As Fe3O4 nanoparticle and AOA are tens of thousands of times less expensive than monoclonal antibodies used in traditional sandwich immunoassays, and the total analysis time of the assay was less than 1 h, the current method affords promise as a biosensor platform that can be adapted for portable and cost-effective detection applications. In a traditional enzyme-linked immunosorbent assay (ELISA) or the immuno-based SERS detection of melamine, monoclonal or polyclonal antibodies are inevitably used to establish the assay [14,27,28]. In comparison with commercial Fe 3 O 4 nanoparticles, 5-aminoorotic acid, and Au nanoparticles, these antibodies are often tens of thousands of times more expensive and require careful handling and disposal because they are sensitive to temperature and pH value.…”

Section: Discussionmentioning

confidence: 99%

“…The regression of the logarithmic peak intensity at 1510 cm −1 versus the melamine concentration produced a linear plot with linear equation as lgI = 2.02148 + 0.05852 × C (lgI represents the logarithms of the peak intensity at 1510 cm −1 , C is the melamine concentrations) and R 2 = 0.9852 in the melamine concentration ranging from 2.5 to 15.0 µg/mL. In a traditional enzyme-linked immunosorbent assay (ELISA) or the immuno-based SERS detection of melamine, monoclonal or polyclonal antibodies are inevitably used to establish the assay [14,27,28]. In comparison with commercial Fe3O4 nanoparticles, 5-aminoorotic acid, and Au nanoparticles, these antibodies are often tens of thousands of times more expensive and require careful handling and disposal because they are sensitive to temperature and pH value.…”

Section: Detection Of Melamine In Milk Using [Fe3o4/au-aoa•••melaminementioning

confidence: 99%

A Fast and Cost-Effective Detection of Melamine by Surface Enhanced Raman Spectroscopy Using a Novel Hydrogen Bonding-Assisted Supramolecular Matrix and Gold-Coated Magnetic Nanoparticles

et al. 2017

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Abstract:A fast and cost-effective melamine detection approach has been developed based on surface enhanced Raman spectroscopy (SERS) using a novel hydrogen bonding-assisted supramolecular matrix. The detection utilizes was formed due to the strong multiple hydrogen bonding interactions between AOA and melamine. The complex was separated and concentrated to a pellet by an external magnet and used as a supramolecular matrix for the melamine detection. Laser excitation of the complex pellet produced a strong SERS signal diagnostic for RhB. The logarithmic intensity of the characteristic RhB peaks was found to be proportional to the concentration of melamine with a limit of detection of 2.5 µg/mL and a detection linearity range of 2.5~15.0 µg/mL in milk. As Fe 3 O 4 nanoparticles and AOA are thousands of times less expensive than the monoclonal antibody used in a traditional sandwich immunoassay, the current assay drastically cut down the cost of melamine detection. The current approach affords promise as a biosensor platform that cuts down sample pre-treatment steps and measurement expense.

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“…) and enzyme‐linked immunosorbent assay (Zhou et al . ) are the popular techniques for the accurate detection of melamine. Although these methods have been widely applied with significant achievements and high accuracy, expensive instruments have to be equipped and the complicated pretreatment process is required as well.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, developing a simple and effective method for the analysis of MEL in these milk products is urgently required. Until now, high-performance liquid chromatography (HPLC) (Wang et al 2013), mass spectrometry ), liquid chromatography/mass spectrometry (Wang et al 2012) and enzyme-linked immunosorbent assay (Zhou et al 2012) are the popular techniques for the accurate detection of melamine. Although these methods have been widely applied with significant achievements and high accuracy, expensive instruments have to be equipped and the complicated pretreatment process is required as well.…”

Section: Introductionmentioning

confidence: 99%

Simple Pretreatment and Portable UV‐Vis Spectrum Instrument for the Rapid Detection of Melamine in Milk Products

Xie

Huang

Yun

et al. 2015

Journal of Food Quality

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In this paper, we report a simple and reliable method for detection of melamine (MEL) in the field of milk products safety screening. This method is developed based on gold nanoparticle (AuNP) probe as the presence of MEL can induce the AuNPs to aggregate and make its color change from wine red to purple. This color change can be quantitatively recorded by a portable UV-vis spectrophotometer so as to calculate the amount of MEL simultaneously. Herein, alcohol is used as MEL extraction solvent without other complex sample processing method because alcohol can easily denature the casein in milk. Furthermore, under the optimized condition, only about 15 min for whole detection process is required to determine the amount of MEL in milk, and the detection limit of MEL can be as low as 2 ppm. PRACTICAL APPLICATIONSAt present, analysis of melamine (MEL) in these milk products is urgent and required. However, most of the published assays require expensive instruments and complicated pretreatment. Herein, a reliable and simple method for MEL detection for field screening of milk was developed, which is based on gold nanoparticle probe with a portable UV-vis spectrophotometer. Herein, ethanol was used as an environmentally friendly solvent to extract MEL from milk, and the whole detection process only required about 15 min.

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Monoclonal antibody based inhibition ELISA as a new tool for the analysis of melamine in milk and pet food samples

Cited by 51 publications

References 27 publications

The Feasibility of Using Near-Infrared Spectroscopy and Chemometrics for Untargeted Detection of Protein Adulteration in Yogurt: Removing Unwanted Variations in Pure Yogurt

The Feasibility of Using Near-Infrared Spectroscopy and Chemometrics for Untargeted Detection of Protein Adulteration in Yogurt: Removing Unwanted Variations in Pure Yogurt

A Fast and Cost-Effective Detection of Melamine by Surface Enhanced Raman Spectroscopy Using a Novel Hydrogen Bonding-Assisted Supramolecular Matrix and Gold-Coated Magnetic Nanoparticles

Simple Pretreatment and Portable UV‐Vis Spectrum Instrument for the Rapid Detection of Melamine in Milk Products

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