Monoclonal antibody‐based method to quantify Gly m 1. Its application to assess environmental exposure to soybean dust

Abstract

A specific and sensitive method is presented that can be used for the quantification of Gly m 1. The application of this method may allow the establishment of risk limits for soybean dust, and thus may contribute to the control of environmental contamination and to the prevention of new asthma epidemics.

Occupational asthma caused by soybean flour in bakers—differences with soybean‐induced epidemic asthma

“…The content of the major soybean hull allergen Gly m 1 was measured in the soybean flour and soybean hull extracts and in different baking additives containing soybean flour by using a monoclonal antibody‐based sandwich ELISA (detection limit < 0.2 ng/mL) as previously described [ 15]. The baking additives handled by the patients: Lecibrem complet (Lecibrem, Madrid, Spain) and Tolerant (Ireks‐Arcady, Kulmbach, Germany) contain a mixture of ingredients, including soybean flour, fungal α‐amylase, malt flour and several preservatives.…”

Quantification of the Major Allergen from Cypress <i>(Cupressus arizonica)</i> Pollen, Cup a 1, by Monoclonal Antibody-Based ELISA

“…This result confirms that this allergen is an important allergen in cypress extracts and indicates that the described assay could be very useful for the standardization of whole extracts in terms of their Cup a 1 allergen content and of their overall IgE-binding activity. Similarly, good correlations between mAb immunoassays and IgE-binding activity have been described in other extracts [14, 17, 25, 26, 27]. There is evidence that the optimal maintenance dose for specific immunotherapy with some allergens is 5–20 µg [11]but this range should not necessarily be followed as a general rule, especially for highly reactive allergens such as Alt a 1, Par j 1 or Cup a 1.…”

Assessment of soy aeroallergen levels in different work environments

“…On the other hand, soy-induced occupational asthma is probably not exclusively caused by soy flour [11]. Several immunoassays have been described for quantifying airborne levels of the LMW soy allergens [7,12,13]. A human IgE enzyme immunoassay (EIA)-inhibition method is currently used for daily monitoring of the soy hull LMW (SHLMW) aeroallergens in the city of Barcelona [12].…”