Monoclonal Antibody Detects a Common Surface Antigen on Two Independently Established Murine Moloney Sarcoma Virus Non-producer Transformants

Lactate Dehydrogenase-elevating Virus Induces Anti-Golgi Apparatus Antibodies

“…After 48 and 68 h the serum of the infected mice was collec'~ed by orbital puncture. Two strains of LDV (LDVRow and LDVpLA) were kindly given by T. Inada, Guy's Hospital Medical School, London, U.K. Titration of AGIA was performed using a recently described bioassay, based on the regular development of AGIA-induced autoantibodies against the GA antigen of cells from different species and against the surface antigen of the Mo-MSV NP transformant Sac (Weiland & Weiland, 1985). The titre of AGIA was determined by injecting i.m.…”

“…AGIA was regularly maintained by acellular transmission in vivo in STU mice. It was isolated originally from the progressor Mo-MSV tumour derivative PV-T-112 (Weiland & Weiland, 1985). Stocks of AGIA were prepared by infecting STU mice intramuscularly (i.m.)…”

“…During our studies of the mouse system we demonstrated that it was not the turnout itself which induced GA autoantibodies, but a transmissible agent found to be associated with progressor-Moloney murine sarcoma virus (Mo-MSV) transformants that had been serially passaged in vivo. It was lost after growth of the tumour cells in culture (Weiland & Weiland, 1985). Characteristically, this agent (tentatively designated anti-GA-inducing agent, AGIA) has the capacity to induce two groups of antibodies: one group which can be demonstrated by indirect immunofluorescence to react with intracellular antigen located in the GA of mammalian, avian and piscine cells (Weiland et al, 1984); and another group, which reacts with surface antigen of the Mo-MSV non-producer (NP) transformant Sac.…”

“…Characteristically, this agent (tentatively designated anti-GA-inducing agent, AGIA) has the capacity to induce two groups of antibodies: one group which can be demonstrated by indirect immunofluorescence to react with intracellular antigen located in the GA of mammalian, avian and piscine cells (Weiland et al, 1984); and another group, which reacts with surface antigen of the Mo-MSV non-producer (NP) transformant Sac. After removal of cytotoxic antibodies by absorption with Sac cells, the GA-reactive antibodies remained demonstrable (Weiland & Weiland, 1985). Sac cells express a cell surface glycoprotein (46K antigen) which is presumably a sarcoma virus-induced cellular antigen that is not immunogenic on the surface of the NP cells (Weiland & Thiel, 1985).…”

“…After removal of cytotoxic antibodies by absorption with Sac cells, the GA-reactive antibodies remained demonstrable (Weiland & Weiland, 1985). Sac cells express a cell surface glycoprotein (46K antigen) which is presumably a sarcoma virus-induced cellular antigen that is not immunogenic on the surface of the NP cells (Weiland & Thiel, 1985). Both groups of AGIAinduced antibodies must be defined as autoantibodies directed against distinct antigens.…”