1988
DOI: 10.1111/j.1600-0897.1988.tb00228.x
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Monoclonal Antibody GB24 Recognizes a Trophoblast‐Lymphocyte Cross‐Reactive Antigen

Abstract: GB24 is a mouse monoclonal antibody (IgG1) raised against human term placental microvilli. This antibody displayed pan-trophoblast reactivity pattern. In addition, GB24 recognized normal peripheral leukocytes and transformed cell lines (Daudi, HL-60, Jurkat, AV3, BeWo, HT-29) by using membrane immunofluorescence and flow cytometry. By SDS-polyacrylamide gel electrophoresis, this antibody immunoprecipitated two proteins of 62 kilodaltons (kDa) and 75 kDa from placental microvilli. Isoelectrofocusing analysis re… Show more

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Cited by 54 publications
(26 citation statements)
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“…Mouse monoclonal antibodies (clones GB24 and TRA-2-10) and rabbit polyclonal antibodies to human CD46 have been described elsewhere (14,30,37,59). Mouse monoclonal antibodies (clone E4.3) against human CD46 were purchased from Ancell, Bayport, Minn.…”
Section: Methodsmentioning
confidence: 99%
“…Mouse monoclonal antibodies (clones GB24 and TRA-2-10) and rabbit polyclonal antibodies to human CD46 have been described elsewhere (14,30,37,59). Mouse monoclonal antibodies (clone E4.3) against human CD46 were purchased from Ancell, Bayport, Minn.…”
Section: Methodsmentioning
confidence: 99%
“…MC57 cells expressing the CD46-BC1 isoform were generated by calcium phosphate transfection ofplasmid K5-23 (11,13), followed by selection with G418 (Geneticin, GIBCO/BRL) at 0.4 mg/ml. Expression of CD46 in the MC57 cells was confirmed by immunofluorescence assay using a monoclonal antibody (GB24) against CD46 (14).…”
mentioning
confidence: 99%
“…GB24 [28] (IgG1; a kind gift of Prof. Atkinson, Washington University, St Louis, MO), directed against CD46; MBC1 (IgG1; hybridoma cells were kindly provided by Prof. B. P. Morgan, University of Wales, Cardiff, GB) and Bric216 (IgG1, IBGRL, Bristol, GB), both directed against CD55; Bric229 (IgG2b; IBGRL) directed against CD59; goat anti-mouse-IgG/M-FITC (DAKO, Glostrup, Denmark) and goat-anti-Human-C3c-FITC (Nordic Immunologicals, Tilburg, The Netherlands). 323/A3(IgG2a)*MBC1 (bispecific mAb), quadroma cells producing this bispecific mAb were developed in our own department as described by Blok et al [14].…”
Section: Antibodiesmentioning
confidence: 99%