Monoclonal antibody stains oligodendrocytes and Schwann cells in zebrafish ( Danio rerio )

Tomizawa, Kaori; Inoue, Yasushi; Doi, Satoko; Nakayasu, Hiroshi

doi:10.1007/s004290050327

Cited by 18 publications

(12 citation statements)

References 24 publications

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“…Synaptic membranes from zebrafish brain were prepared by the method used to prepare bovine brain synaptic membrane fractions (Tomizawa, 2000a and2000b). Zebrafish were maintained at 28.5 o C under a 14h light and 10h dark cycle (Westerfield, 1995).…”

Section: Preparation Of the Synaptic Membrane Fraction From Zebrafishmentioning

confidence: 99%

Distribution of a brain-specific extracellular matrix protein in developing and adult zebrafish

2007

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Section: Preparation Of the Synaptic Membrane Fraction From Zebrafishmentioning

confidence: 99%

Distribution of a brain-specific extracellular matrix protein in developing and adult zebrafish

2007

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“…Briefly, a zebrafish head was fixed in FX buffer (Tomizawa et al, 2000a), containing 4% freshly depolymerized paraformaldehyde at 4°C overnight. The brain was carefully removed from the skull and gently washed in 0.1 M sodium phosphate buffer (pH 7.3) containing 4% sucrose and 0.15 mM CaCl 2 and then placed into a 30% sucrose solution until it sank.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Three‐dimensional distribution of astrocytes in zebrafish spinal cord

Kawai¹,

Arata²,

Nakayasu³

2001

Glia

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We prepared a monoclonal antibody (A-22) that recognizes a 60-kDa protein in the zebrafish brain. The antigen is distributed throughout the brain but is not found outside it. The antibody recognizes star-shaped cells with long processes in the spinal cord. All A-22-positive cells are also GFAP-immunopositive, but there are GFAP-positive cells that are A-22-negative. The cells are connected to small veins and to the surface of the spinal cord. Immunopositive cells are generally homogeneous in size and shape and are found not only in the spinal cord but also in several areas of the brain. These results indicate that the stained cell is an astrocyte. Most of these cells (88%) are distributed in the gray matter of the spinal cord; the remainder (12%) are found in the white matter. Most of the cells in the gray matter are found in the ventral and dorsal horns, but some are also present in the central area along the ventricle. Glial cell bodies form an array along the longitudinal axis and are connected to each other by thick projections. The cellular array is not visible in coronal sections. In contrast, thin processes from the cells extend to the surfaces of veins, to neurons, and to the periphery of the spinal cord. We estimate that there are about 13,500 A-22-positive astrocytes in the spinal cord; however, this represents only 26% of the total number of astrocytes in the spinal cord (approximately 52,000).

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“…Zebrafish (Danio rerio) were kept and raised according to standard conditions (Westerfield 1995, Tomizawa 2000a. Fertilization was carried out according to the conditions listed in "The zebrafish book, 3rd edition" (Westerfield 1995).…”

Section: Animalsmentioning

confidence: 99%

“…For immunohistochemical studies rabbit polyclonal anti-human myelin basic protein (MBP) was obtained from YLEM and was used at a 2:3 dilution (Tomizawa 2000a).…”

Section: Mbp Primary Antibodymentioning

confidence: 99%