Monoclonal Antibody to Streptococcal Group B Carbohydrate: Applications in Latex Agglutination and Immunoprecipitin Assays

Ruch, Frank E.; Smith, L P

doi:10.1128/jcm.16.1.145-152.1982

Cited by 8 publications

(1 citation statement)

References 20 publications

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“…Theoretically, the ability of the monoclonal LA reagent to detect smaller quantities of GBS antigen (31.25 versus 62.5 ng/ml) (2) and to be more specific than polyclonal LA reagents may make it the method of choice. Although monoclonal antibody to GBS bound to latex microspheres has been previously evaluated as a potentially useful diagnostic reagent (9), Directigen provides the first such reagent which is commercially available. This availability combined with its excellent sensitivity and specificity make the Directigen GBS test a useful reagent for the rapid diagnosis of invasive GBS infection in neonates and young infants.…”

Section: Discussionmentioning

confidence: 99%

Detection of group B streptococcal antigen in body fluids by a latex-coupled monoclonal antibody assay

Rench¹,

Metzger²,

Baker³

1984

J Clin Microbiol

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A commercially available latex agglutination reagent, Directigen (Hynson, Westcott & Dunning, Div. Becton Dickinson & Co., Baltimore, Md.), in which a murine monoclonal antibody to group B streptococcal (GBS) antigen is the active component, was evaluated by using body fluid specimens from 94 sick infants. Antigen was detected in one or more admission specimens from 18 of 19 (94.7%) infants with symptomatic GBS infection. In 15 patients with GBS meningitis, cerebrospinal fluid, serum, and 10-fold-concentrated urine specimens were positive in 87, 50, and 100%, respectively. GBS antigen was detected in 50% of unconcentrated urines, but in no sera from infants with nonmeningitic GBS sepsis. Among specimens from 27 infants with invasive infection due to organisms other than GBS and from 48 culture-negative sick infants, false-positive latex agglutination reactions occurred in only 4 (9.5%) urine specimens and in no cerebrospinal fluid or serum specimens. The 94.7% sensitivity and specificity of the Directigen GBS test indicate that it is a useful reagent for the rapid diagnosis of invasive GBS infection in young infants.

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Section: Discussionmentioning

confidence: 99%

Detection of group B streptococcal antigen in body fluids by a latex-coupled monoclonal antibody assay

Rench¹,

Metzger²,

Baker³

1984

J Clin Microbiol

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Characterization of Cross-Reactivity Between Cryptococcal Polysaccharides By Use of Enzyme-Linked Immunosorbent Assays and Monoclonal Antibodies

Kozel

Eckert

1988

Fungal Antigens

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Evaluation of a rapid latex agglutination test for detection of group B streptococci in vaginal specimens

Lotz-Nolan

Amato

Iltis

et al. 1989

Eur. J. Clin. Microbiol. Infect. Dis.

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A rapid latex agglutination test (Bactigen Group B Streptococcus Cervical Screen) for detection of group B streptococci in cervical-vaginal specimens was evaluated using two different slide systems, the traditional serologic slide and capillary action track (Trak) slide. Culture was used as reference method. A total of 344 cervical-vaginal specimens were tested. The group B streptococci carrier rate was found by culture to be 10.8%, 56.8% of these specimens being heavily colonized. The sensitivity and specificity of the latex agglutination test in heavily colonized specimens was 95.2% and 99.3% for the serologic and track slides respectively. The overall sensitivity, including lightly colonized specimens, was 62.2%. The positive predictive value was 92% for both slide systems, and the negative predictive value 95.4% and 95.6% for the serologic and track slides respectively. The latex agglutination test, used with either slide, provides a rapid and effective method for identification of specimens heavily colonized with group B streptococci. The track slide may provide a convenient alternative to serologic slides since it does not require rotation.

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Monoclonal Antibody to Streptococcal Group B Carbohydrate: Applications in Latex Agglutination and Immunoprecipitin Assays

Cited by 8 publications

References 20 publications

Detection of group B streptococcal antigen in body fluids by a latex-coupled monoclonal antibody assay

Detection of group B streptococcal antigen in body fluids by a latex-coupled monoclonal antibody assay

Characterization of Cross-Reactivity Between Cryptococcal Polysaccharides By Use of Enzyme-Linked Immunosorbent Assays and Monoclonal Antibodies

Evaluation of a rapid latex agglutination test for detection of group B streptococci in vaginal specimens

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