Monoclonal BALB/c anti‐progesterone antibodies use family IX variable region heavy chain genes

Deverson, Edward V.; Berek, Claudia; Taussig, Michael J.; Feinstein, A.

doi:10.1002/eji.1830170103

Cited by 30 publications

(20 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, they are found in several strains of mice and code for plasmacytomas as well as for elicited or auto antibodies. For instance, the V, 5.1 gene encodes the Abl' antibodies of the GAT cascade ) and antibodies against digoxin (Novotny & MargoUes 1983), DNA (Kofler et al 1985), dinitrophenol (Hum et al 1984), progesterone (Deverson et al 1987), and alprenolol (Nahmias et al 1988). The V« 1A5 gene encodes the Abl of the GAT cascade , as well as antibodies against lysozyme (Darsley & Rees 1985), flagellin ( Hum et al 1984), and phenyloxazolone (Kaartinen et al 1986).…”

Section: The Vhw Germline Genementioning

confidence: 99%

Breaking The First Circle

Fougereau¹,

Scmff²

1988

Immunological Reviews

View full text Add to dashboard Cite

Section: The Vhw Germline Genementioning

confidence: 99%

Breaking The First Circle

Fougereau¹,

Scmff²

1988

Immunological Reviews

View full text Add to dashboard Cite

“…Messenger RNA sequencing of several anti-progesterone mAbs from our panel, including DB3, has shown that VH and VL are derived from a restricted set of V genes (23,24). The structural relatedness of anti-progesterone antibodies is reflected in the cross-reaction of the rabbit anti-DB3-Id with different members of the panel (Fig.…”

Section: Discussionmentioning

confidence: 97%

Blocking of pregnancy in mice by immunization with anti-idiotype directed against monoclonal anti-progesterone antibody.

Wang

Heap

Taussig

1989

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Passive transfer of monoclonal anti-progesterone antibodies shortly after mating blocks the onset of pregnancy in different species (mouse, rat, and ferret). Here we report that BALB/c mice can be actively immunized against progesterone, and hence against pregnancy, by means of rabbit anti-idiotypic antibodies specific for a mouse monoclonal antiprogesterone antibody, DB3. Some of the anti-idiotypic antibodies reacted with the steroid-combining site on the DB3 molecule. In response to repeated anti-idiotypic immunization, mice produced serum anti-progesterone antibodies (up to 100 ,ug/ml) that resembled DB3 in idiotypy, affinity, and specificity for progesterone and other steroid ligands. Thus an anti-idiotype can mimic the antigenicity of a steroid hormone with a high degree of accuracy. Compared with immunization with a progesterone-bovine serum albumin conjugate, the anti-progesterone response to anti-idiotype was considerably lower and clonally restricted. When mated after completion of the immunization course, the fertility rate of anti-idiotypeimmunized mice was reduced to 30% from a control level of 91%. The anti-fertility effect was correlated with the circulating anti-progesterone concentration in individual animals and persisted for 4 or 5 estrous cycles. Active immunization with progesterone-bovine serum albumin was a highly effective means of rendering mice infertile; it reduced the fertility rate to zero over 16 or 17 estrous cycles. Our results suggest that anti-idiotypes may form the basis of contraceptive vaccines.Immunological intervention in pregnancy is of interest as a possible approach to contraception and the control of reproduction. The targets for such intervention include the hormones associated with pregnancy and antigens on gametes. Thus, recent work has focused on a vaccine against human chorionic gonadotrophin (1) and immunization against sperm-specific proteins (2). Progesterone, the key steroid hormone required for the establishment and maintenance of pregnancy, is another possible target molecule. Active antiprogesterone immunization with a progesterone-protein complex blocks pregnancy in rats (3) and rabbits (4), and we have shown that passive immunization with anti-progesterone monoclonal antibodies (mAbs) shortly after mating prevents implantation and pregnancy in mice (5, 6), rats (7), and ferrets (8). The action of anti-progesterone antibody involves sequestration of the hormone in the circulation, preventing it from reaching progesterone receptors in uterine tissue (9); in addition, we have recently shown that antibody becomes localized specifically on the surface of progesterone-sensitive areas of the uterus (luminal and glandular epithelium) just before the time of implantation, indicating the possibility of a local blocking action affecting hormone uptake (10).Anti-idiotypes (Id) are antibodies that recognize the antigen-combining regions of other antibodies and as such have been widely used as antigen mimics and experimental vaccines (11,12). A possible route to ...

show abstract

“…The comparison of their VH sequences showed many amino acid substitutions. This suggests that several members can be used [38]. On the other hand, the comparison of VGAM3.8 sequences of h2 (VxJ2) hybridomas of mouse E showed a quasi nucleotide identity with the sequence of H130.5 [39] (Fig.…”

Section: Individual Vhna Biases Are Reinforced By the Use Of Limited mentioning

confidence: 94%

Available λ B cell repertoire in the mouse: Evidence of positive selection by environmental factors

et al. 1993

View full text Add to dashboard Cite

We have recently shown that, from two BALB/c mice treated with rabbit anti-C lambda 2/C lambda 3 antibodies coupled to lipopolysaccharide, variable heavy chain (VH) family repertoires associated with lambda 2 or lambda 3 light chains can differ from one lambda subtype to another and from one individual mouse to another. Indeed, 4 out of 6 lambda 2 (VxJ2) hybridomas from one mouse preferentially expressed the VH10 family while 3 out of 8 lambda 2 (V2J2) and 5 out of 8 lambda 2 (VxJ2) hybridomas from a second mouse preferentially expressed the S107 and VGAM3.8 VH families, respectively. In this report, we describe the structural basis of such preferential pairings by sequence analysis of the 12 lambda 2 hybridomas. The sequence comparison of their VH regions show that each preferential association of a VH family to one V lambda region is restricted to the use of a single member or very closely related members inside a VH family and that a great variability of CDR3 of heavy chain is observed. We, therefore, suggest that environmental factors can modify the available lambda B cell repertoire through a positive selection of particular VH/V lambda pairings. Moreover, our data support that this selection does not require clonal expansion and punctual somatic mutation.

show abstract

Monoclonal BALB/c anti‐progesterone antibodies use family IX variable region heavy chain genes

Cited by 30 publications

References 27 publications

Breaking The First Circle

Breaking The First Circle

Blocking of pregnancy in mice by immunization with anti-idiotype directed against monoclonal anti-progesterone antibody.

Available λ B cell repertoire in the mouse: Evidence of positive selection by environmental factors

Contact Info

Product

Resources

About