Monocyte Chemoattractant Protein-1 stimulates the differentiation of rat stem and progenitor Leydig cells during regeneration

Zhan, Xiangcheng; Zhang, Jingwei; Li, Saiyang; Zhang, Xiaolu; Li, Fulin; Song, Ting; Liu, Qunlong; Lu, Jun; Xu, Yunfei; Ge, Ren‐Shan

doi:10.1186/s12861-020-00225-1

Cited by 3 publications

(2 citation statements)

References 40 publications

(81 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…D medium (consisting of PBGF-BB, LH, thyroid hormone, and IGF-1) was previously reported by Ge et al [31]. D medium is widely used to induce the differentiation of SLCs into LCs [5,18,19,[32][33][34][35]. F medium contains forskolin (FSK), which is a stimulator of adenylate cyclase that results in increased adenosine-3 0 ,5cyclic monophosphate (cAMP) production [36].…”

Section: Htscs Can Differentiate Into Llcs In Vitromentioning

confidence: 99%

Rapid Differentiation of Human Embryonic Stem Cells into Testosterone-Producing Leydig Cell-Like Cells In vitro

Shin

Park

Choi³

et al. 2021

Tissue Eng Regen Med

View full text Add to dashboard Cite

Background: Leydig cells (LCs) are testicular somatic cells that are the major producers of testosterone in males. Testosterone is essential for male physiology and reproduction. Reduced testosterone levels lead to hypogonadism and are associated with diverse pathologies, such as neuronal dysfunction, cardiovascular disease, and metabolic syndrome. LC transplantation is a promising therapy for hypogonadism; however, the number of LCs in the testis is very rare and they do not proliferate in vitro. Therefore, there is a need for an alternative source of LCs. Methods: To develop a safer, simple, and rapid strategy to generate human LC-like cells (LLCs) from stem cells, we first performed preliminary tests under different conditions for the induction of LLCs from human CD34/CD73 double positive-testis-derived stem cells (HTSCs). Based on the embryological sequence of events, we suggested a 3-step strategy for the differentiation of human ESCs into LLCs. We generated the mesendoderm in the first stage and intermediate mesoderm (IM) in the second stage and optimized the conditions for differentiation of IM into LLCs by comparing the secreted testosterone levels of each group. Results: HTSCs and human embryonic stem cells can be directly differentiated into LLCs by defined molecular compounds within a short period. Human ESC-derived LLCs can secrete testosterone and express steroidogenic markers. Conclusion: We developed a rapid and efficient protocol for the production of LLCs from stem cells using defined molecular compounds. These findings provide a new therapeutic cell source for male hypogonadism.

show abstract

Section: Htscs Can Differentiate Into Llcs In Vitromentioning

confidence: 99%

Rapid Differentiation of Human Embryonic Stem Cells into Testosterone-Producing Leydig Cell-Like Cells In vitro

Shin

Park

Choi³

et al. 2021

Tissue Eng Regen Med

View full text Add to dashboard Cite

show abstract

“…TP53, a master transcription factor, plays a role in suppressing cell proliferation by upregulating CDKN1B levels [ 14 ]. Moreover, factors such as pAKT1, pERK1/2, and pCREB have been implicated in the activation of cell proliferation [ 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Patulin Stimulates Progenitor Leydig Cell Proliferation but Delays Its Differentiation in Male Rats during Prepuberty

Li,

Su,

Lin

et al. 2023

Toxins

View full text Add to dashboard Cite

Patulin is a mycotoxin with potential reproductive toxicity. We explored the impact of patulin on Leydig cell (LC) development in male rats. Male Sprague Dawley rats (21 days postpartum) were gavaged patulin at doses of 0.5, 1, and 2 mg/kg/day for 7 days. Patulin markedly lowered serum testosterone at ≥0.5 mg/kg and progesterone at 1 and 2 mg/kg, while increasing LH levels at 2 mg/kg. Patulin increased the CYP11A1+ (cholesterol side-chain cleavage, a progenitor LC biomarker) cell number and their proliferation at 1 and 2 mg/kg. Additionally, patulin downregulated Lhcgr (luteinizing hormone receptor), Scarb1 (high-density lipoprotein receptor), and Cyp17a1 (17α-hydroxylase/17,20-lyase) at 1 and 2 mg/kg. It increased the activation of pAKT1 (protein kinase B), pERK1/2 (extracellular signal-related kinases 1 and 2), pCREB (cyclic AMP response binding protein), and CCND1 (cyclin D1), associated with cell cycle regulation, in vivo. Patulin increased EdU incorporation into R2C LC and stimulated cell cycle progression in vitro. Furthermore, patulin showed a direct inhibitory effect on 11β-HSD2 (11β-hydroxysteroid dehydrogenase 2) activity, which eliminates the adverse effects of glucocorticoids. This study provides insights into the potential mechanisms via which patulin affects progenitor LC development in young male rats.

show abstract

Stem Leydig cells: Current research and future prospects of regenerative medicine of male reproductive health

Tian²,

Wang

et al. 2022

Seminars in Cell & Developmental Biology

View full text Add to dashboard Cite

Monocyte Chemoattractant Protein-1 stimulates the differentiation of rat stem and progenitor Leydig cells during regeneration

Cited by 3 publications

References 40 publications

Rapid Differentiation of Human Embryonic Stem Cells into Testosterone-Producing Leydig Cell-Like Cells In vitro

Rapid Differentiation of Human Embryonic Stem Cells into Testosterone-Producing Leydig Cell-Like Cells In vitro

Patulin Stimulates Progenitor Leydig Cell Proliferation but Delays Its Differentiation in Male Rats during Prepuberty

Stem Leydig cells: Current research and future prospects of regenerative medicine of male reproductive health

Contact Info

Product

Resources

About