Monocyte-derived dendritic cells from late gestation cows have an impaired ability to mature in response to E. coli stimulation in a receptor and cytokine-mediated fashion

Pomeroy, Brianna; Sipka, Anja; Klaessig, Suzanne; Schukken, Y.H.

doi:10.1016/j.vetimm.2015.06.016

Cited by 10 publications

(28 citation statements)

References 43 publications

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“…We found previously that relative to nonpregnant cows, monocyte-derived DC (moDC) from cows in late gestation had hindered phenotypic maturation and altered cytokine secretion in response to E. coli stimulation and thus were likely to have a poor ability to activate T cells during late gestation. We also showed that these moDC from cows in late gestation showed heightened IL-10 production and hindered MHC II upregulation (Pomeroy et al, 2015). Hormone profiles unique to late gestation may affect the ability of DC to prime and activate T cells during this stage of pregnancy.…”

Section: Introductionmentioning

confidence: 67%

“…Across multiple species dendritic cells (DC) are known to play a crucial role in generating maternal immune tolerance toward fetal antigens by preventing the development of highly inflammatory, fetal antigen-specific responses (Zenclussen et al, 2005, Kahn and Baltimore, 2010, Petroff, 2011. Our previous work indicated that these cells are regulated in bovine late gestation (Bachy et al, 2008, Della Bella et al, 2011, Negishi et al, 2012, Leno-Duran et al, 2014, Pomeroy et al, 2015. We found previously that relative to nonpregnant cows, monocyte-derived DC (moDC) from cows in late gestation had hindered phenotypic maturation and altered cytokine secretion in response to E. coli stimulation and thus were likely to have a poor ability to activate T cells during late gestation.…”

Section: Introductionmentioning

confidence: 99%

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Impact of in vitro treatments of physiological levels of estradiol and progesterone observed in pregnancy on bovine monocyte-derived dendritic cell differentiation and maturation

Pomeroy

Klaessig

Schukken

2016

Veterinary Immunology and Immunopathology

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The specific factors which regulate differentiation and maturation of dendritic cells in bovine pregnancy remain unclear. We evaluated the influence of physiologically relevant in vitro treatments of progesterone (PG) and estradiol (E2) observed in late pregnancy on the differentiation and maturation of CD14+ monocyte-derived dendritic cell (moDC) from non-pregnant, lactating dairy cows (n=7). We found that moDC differentiated in the presence of both E2 and PG had impaired E. coli-induced phenotypic maturation, specifically a significant reduction in CD80 and MHC II expression. Contrary to our previous work characterizing moDC from late gestating dairy cattle, we did not observe an increase in CD14 expression relative to the untreated control; this increase was only observed in the current data in the dexamethasone-treated moDC. The moDC treated with a combination of both E2 and PG had significantly greater upregulation of anti-inflammatory cytokine IL-10 relative to the untreated control, but TNFα production was not suppressed; only dexamethasone-treated moDC showed abrogated TNFα production. These data suggest moDC may be regulated by E2 and PG to hinder phenotypic maturation and regulate inflammatory responses. Pregnancy-associated hormone profiles appear to be involved in the generation of maternal immune tolerance in pregnancy. These hormone-facilitated changes to moDC in pregnancy may also impede optimal immune responses to both invading pathogens and routine vaccinations administered in late gestation through limited antigen presentation and increased anti-inflammatory cytokine production. These results provide insight into maternal immune modulation and elucidate potential immune changes necessary to facilitate bovine pregnancy.

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Section: Introductionmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Impact of in vitro treatments of physiological levels of estradiol and progesterone observed in pregnancy on bovine monocyte-derived dendritic cell differentiation and maturation

Pomeroy

Klaessig

Schukken

2016

Veterinary Immunology and Immunopathology

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“…In contrast, a half of cattle in this study showed low expressions of CD205, CD80 and CD86 with low expressions of IL‐12a , IL‐4 , IFN‐ γ and IL‐6 . A previous study indicates that bovine DCs in late gestation have reduced Th1‐promoting cytokine production compared with regulatory cytokine production (Pomeroy, Sipka, Klaessig, & Schukken, 2015). Therefore, a half of bovine peripheral DCs before parturition may be immature and tend to maintain tolerance based on the low cytokine production.…”

Section: Discussionmentioning

confidence: 98%

Phenotypic and functional analysis of bovine peripheral blood dendritic cells before parturition by a novel purification method

Zhuang

Urakawa

Sato

et al. 2018

Animal Science Journal

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Dendritic cells (DCs) are specialized antigen presenting cells specializing in antigen uptake and processing, and play an important role in the innate and adaptive immune response. A subset of bovine peripheral blood DCs was identified as CD172a+/CD11c+/MHC (major histocompatibility complex) class II + cells. Although DCs are identified at 0.1%–0.7% of peripheral blood mononuclear cells (PBMC), the phenotype and function of DCs remain poorly understood with regard to maintaining tolerance during the pregnancy. All cattle used in this study were 1 month before parturition. We have established a novel method for the purification of DCs from PBMC using magnetic‐activated cell sorting, and purified the CD172a+/CD11c+ DCs, with high expression of MHC class II and CD40, at 84.8% purity. There were individual differences in the expressions of CD205 and co‐stimulatory molecules CD80 and CD86 on DCs. There were positive correlations between expression of cytokine and co‐stimulatory molecules in DCs, and the DCs maintained their immune tolerance, evidenced by their low expressions of the co‐stimulatory molecules and cytokine production. These results suggest that before parturition a half of DCs may be immature and tend to maintain tolerance based on the low cytokine production, and the other DCs with high co‐stimulatory molecules may already have the ability of modulating the T‐cell linage.

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“…On the other hand it turns out to be a high risk period as management of dry cows is often somewhat neglected and the cow in late gestation is reprogramming its immune response toward a Th-2 dominated response or an immune response that is more permissive of infections without mounting a substantial immune response to remove potential invading micro-organisms (Quesnell et al 2012). Several recent studies from our research team have been focused on the immune response during late gestation showing that the both Natural Killer cells and Dendritic Cells play an important role in directing the immune response in late gestation (Pomeroy et al 2015.…”

Section: Mastitis: High Risk Of Infection In Late Gestationmentioning

confidence: 99%

Farm animal health : progress, problems and perspectives

Schukken

Wias²

2017

Preprint

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Monocyte-derived dendritic cells from late gestation cows have an impaired ability to mature in response to E. coli stimulation in a receptor and cytokine-mediated fashion

Cited by 10 publications

References 43 publications

Impact of in vitro treatments of physiological levels of estradiol and progesterone observed in pregnancy on bovine monocyte-derived dendritic cell differentiation and maturation

Impact of in vitro treatments of physiological levels of estradiol and progesterone observed in pregnancy on bovine monocyte-derived dendritic cell differentiation and maturation

Phenotypic and functional analysis of bovine peripheral blood dendritic cells before parturition by a novel purification method

Farm animal health : progress, problems and perspectives

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