Monocyte-derived macrophages from Zebu (Bos taurus indicus) are more efficient to control Brucella abortus intracellular survival than macrophages from European cattle (Bos taurus taurus)

Macêdo, Auricélio A.; Costa, Érica Azevedo; Silva, A.P.C.; Paixão, Tatiane Alves da; Santos, Renato L.

doi:10.1016/j.vetimm.2012.12.001

Cited by 23 publications

(10 citation statements)

References 61 publications

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“…Macrophages from Zebu are more efficient in controlling intracellular replication of Brucella abortus . Furthermore, Zebu macrophages generate higher levels of nitric oxide and cytokines such as IL-12 and TNF-α [ 30 ]. Therefore, increasing lines of evidences support the notion that resistance of Zebu cattle may be due to quantitative and qualitative differences in immune cells.…”

Section: Discussionmentioning

confidence: 99%

Influence of endogamy and mitochondrial DNA on immunological parameters in cattle

et al. 2014

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BackgroundEndogamy increases the risk of manifestation of deleterious recessive genes. Mitochondrial DNA allows the separation of American Zebu (Bos indicus and Bos taurus) and evaluate the effect of mitochondrial DNA on productive traits of cattle. However, the effect of endogamy and mitochondrial DNA (mtDNA) on the immune system remains unclear. The aim of this study was to evaluate the association between endogamy, mtDNA and immune parameters.ResultsA total of 86 cattle (43 cows and 43 calves) were used in this study. Age, endogamy, milk yield, and origin of mtDNA were measured and their influence on immunological parameters was evaluated. Older cows had increased CD4+ T cells, decreased CD21+ and γδhigh T cells as well as increased CD4+/CD8+ and T/B ratio. Multiple regression analysis indicated that endogamy in calves was associated with increased CD8+ T and CD21+ B lymphocytes, and decreased γδhigh T cells in peripheral blood. Cows with medium and lower endogamy had a lower percentage of B lymphocytes and γδlow T cells and cows with lower endogamy had higher levels of γδ T cells and γδhigh T cells, as well as the CD4+/CD48+ cell ratio. Calves with higher endogamy had higher levels of CD8+ T lymphocytes, whereas calves with lower endogamy had lower levels of γδlow T cells.ConclusionsThese results demonstrated for the first time that endogamy influences the immune system of cattle.

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Section: Discussionmentioning

confidence: 99%

Influence of endogamy and mitochondrial DNA on immunological parameters in cattle

et al. 2014

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“…When appropriate, transcript levels were indicated as number of mRNA copies per 1000 copies of GAPDH mRNA. RNA extracted from peripheral blood monocytederived macrophages obtained from a 3-year-old crossbred bull as previously described (Macêdo et al, 2013) was used as a positive control for MHC class I amplification.…”

Section: Chorioallantoic Membrane Explants Culture and Infectionmentioning

confidence: 99%

Transcription of non-classic major histocompatibility complex (MHC) class I in the bovine placenta throughout gestation and after Brucella abortus infection

Santos

Mol

Macêdo

et al. 2015

Veterinary Immunology and Immunopathology

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“…However, macrophages are also important for the persistence of Brucella spp. in the host [ 25 , 26 ]. Therefore, experimental studies in primary macrophages infected with Brucella are important for a full understanding of the pathogenesis and virulence mechanisms of this bacterium, as well as the immune response of the host.…”

Section: Introductionmentioning

confidence: 99%

The abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophages

et al. 2015

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Brucella ovis infection is associated with epididymitis, orchitis and infertility in rams. Most of the information available on B. ovis and host cell interaction has been generated using murine macrophages or epithelial cell lines, but the interaction between B. ovis and primary ovine macrophages has not been studied. The aim of this study was to evaluate the role of the B. ovis abcEDCBA-encoded ABC transporter and the virB operon-encoded Type IV Secretion System (T4SS) during intracellular survival of B. ovis in ovine peripheral blood monocyte-derived macrophages. ΔabcBA and ΔvirB2 mutant strains were unable to survive in the intracellular environment when compared to the WT B. ovis at 48 hours post infection (hpi). In addition, these mutant strains cannot exclude the lysosomal marker LAMP1 from its vacuolar membrane, and their vacuoles do not acquire the endoplasmic reticulum marker calreticulin, which takes place in the WT B. ovis containing vacuole. Higher levels of nitric oxide production were observed in macrophages infected with WT B. ovis at 48 hpi when compared to macrophages infected with the ΔabcBA or ΔvirB2 mutant strains. Conversely, higher levels of reactive oxygen species were detected in macrophages infected with the ΔabcBA or ΔvirB2 mutant strains at 48 hpi when compared to macrophages infected with the WT strain. Our results demonstrate that B. ovis is able to persist and multiply in ovine macrophages, while ΔabcBA and ΔvirB2 mutations prevent intracellular multiplication, favor phagolysosome fusion, and impair maturation of the B. ovis vacuole towards an endoplasmic reticulum-derived compartment.

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Monocyte-derived macrophages from Zebu (Bos taurus indicus) are more efficient to control Brucella abortus intracellular survival than macrophages from European cattle (Bos taurus taurus)

Cited by 23 publications

References 61 publications

Influence of endogamy and mitochondrial DNA on immunological parameters in cattle

Influence of endogamy and mitochondrial DNA on immunological parameters in cattle

Transcription of non-classic major histocompatibility complex (MHC) class I in the bovine placenta throughout gestation and after Brucella abortus infection

The abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophages

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