Monocyte-derived tissue factor contributes to stent thrombosis in an in vitro system

Palmerini, Tullio; Coller, Barry S.; Cervi, Vittorio; Tomasi, Luciana; Marzocchi, Antonío; Marrozzini, Cinzia; Leone, Ornella; Piccioli, Milena; Branzi, Angelo

doi:10.1016/j.jacc.2004.07.028

Cited by 46 publications

(38 citation statements)

References 28 publications

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“…One of the other finding, the presence of tissue factor at sites of fibrin deposition, also provide important information to gain further insight into the role of tissue factor in the formation. Although our finding do not exclude possible contributory role of other coagulation related proteins such as factor Xa [32], and factor XIa [33], our results confirm previously published findings demonstrating the crucial role of tissue factor in the formation of arterial-occlusive thrombus formation with the experimental thrombosis [11][12][13]34,35]. Co-localization of platelet and fibrin shown in our finding also suggested the possible contributory role of activated platelet in the formation of fibrin fibrils previously shown in the experimental conditions [36,37].…”

Section: Discussionsupporting

confidence: 81%

“…Our results represent the first clear demonstration of the co-localization of VWF with platelets, and of tissue factor and platelets with fibrin fibrils, in human coronary thrombi causing acute myocardial infarction, confirming their possible contribution suggested by experimental thrombosis [10][11][12][13]. Characterization of inflammatory cells involved in coronary thrombi by cell surface markers revealed the constant presence of CD45-and CD16-positive cells.…”

Section: Discussionsupporting

confidence: 80%

“…Indeed, several ex vivo studies have demonstrated the possible roles of von Willebrand factor (VWF), platelets, leukocytes, tissue factor, and fibrin deposition in experimental occlusive thrombus formation [10][11][12][13]. However, their roles in the formation of human coronary thrombi causing myocardial infarction are still to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Co‐localization of von Willebrand factor with platelet thrombi, tissue factor and platelets with fibrin, and consistent presence of inflammatory cells in coronary thrombi obtained by an aspiration device from patients with acute myocardial infarction

Hoshiba

Hatakeyama

Tanabe

et al. 2006

Journal of Thrombosis and Haemostasis

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Summary. Background: Detailed histochemical analysis of coronary thrombi obtained freshly from acute phase of myocardial infarction patients may provide information necessary to understand the mechanism of coronary occlusive thrombus formation. Methods and Results: Coronary thrombi causing myocardial infarction were obtained from 10 consecutive patients of myocardial infarction in the acute phase, using a newly developed aspiration catheter. All the fixed specimens of coronary thrombi, by hematoxylin and eosin staining, were found to contain three major constituents, namely, platelets, densely packed fibrin and inflammatory cells, including polymorphonuclear and mononuclear cells, although their distribution in each specimen is totally heterogeneous. Immunohistochemical staining revealed the prominent presence of von Willebrand factor (VWF) at the sites of platelet accumulation, presence of tissue factor and platelets at the sites of deposition of fibrin fibrils. It also revealed the presence of CD16-, CD45-and CD34-positive cells, yet the functional roles of these cells have still to be elucidated. There are weak positive correlation between the number of inflammatory cells involved in the unit area of coronary thrombi specimen and the time of collection of the specimens after the onset of chest pain. Conclusions: In spite of various limitations, our results contain information suggesting the possible role of VWF in platelet-thrombus formation, possible important role played by tissue factor and activated platelets in the formation of fibrin fibrils, and the positive relationship between inflammatory cells migration and the formation of occlusive thrombi in human coronary arteries.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

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Co‐localization of von Willebrand factor with platelet thrombi, tissue factor and platelets with fibrin, and consistent presence of inflammatory cells in coronary thrombi obtained by an aspiration device from patients with acute myocardial infarction

Hoshiba

Hatakeyama

Tanabe

et al. 2006

Journal of Thrombosis and Haemostasis

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“…Leukocytes may have a prothrombotic effect by producing tissue factor, 16,17 by providing a catalytic surface for thrombin generation, 18 and/or by forming plateletleukocyte aggregates. 19 An elevated WBCc may also result in diminished microcirculatory perfusion by adhesion, aggregation, platelet recruitment, 20 and release of proinflammatory and vasculotoxic factors.…”

Section: Discussionmentioning

confidence: 99%

Impact of Leukocyte Count on Mortality and Bleeding in Patients With Myocardial Infarction Undergoing Primary Percutaneous Coronary Interventions

et al. 2011

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Background-The relationship between white blood cell count (WBCc) and mortality in patients with ST-segmentelevation acute myocardial infarction treated with percutaneous coronary intervention is poorly understood. Furthermore, whether there is a relationship between WBCc and risk of noncardiac mortality and bleeding after percutaneous coronary intervention is unknown. Methods and Results-The baseline WBCc was available in 3193 of 3345 patients (95.5%) who underwent percutaneous coronary intervention in the Harmonizing Outcome With Revascularization and Stent in Acute Myocardial Infarction (HORIZONS-AMI) trial. In a propensity-adjusted multivariable analysis, WBCc was an independent predictor of 1-year cardiac mortality (hazard ratio, 1.15; 95% confidence interval, 1.09 to 1.22), noncardiac mortality (hazard ratio, 1.19; 95% confidence interval, 1.10 to 1.29), and major bleeding (hazard ratio, 1.08; 95% confidence interval, 1.04 to 1.12). After adjustment for baseline creatinine phosphokinase levels and left ventricular ejection fraction, WBCc remained an independent predictor of 1-year all-cause mortality and cardiac mortality. In patients matched for baseline creatinine phosphokinase levels at hospital admission, the median peak creatinine phosphokinase level was significantly higher in patients with high WBCc (Ͼ11 000 per 1 mm 3 ) compared with low WBCc (1851 U/L [range, 880-3307 U/L] versus 1241 U/L [range, 540 to 2,78], respectively; PϽ0.0001). In this subgroup of patients, WBCc was an independent correlate of peak creatinine phosphokinase level, and remained an independent predictor of 1-year mortality. Conclusions-In patients with ST-segment-elevation acute myocardial infarction undergoing percutaneous coronary intervention, elevated baseline WBCc is an independent predictor of infarct size, as assessed by peak creatinine phosphokinase level, and of 1-year cardiac mortality, noncardiac mortality, and major bleeding. Clinical Trial Registration-URL: http://www.ClinicalTrials.gov. Unique identifier: NCT00433966.

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“…15 Although generally considered a nonspecific marker of inflammatory processes, leukocytosis is now emerging as a potential factor that may directly contribute to vascular disease. Leukocytes, in fact, may have prothrombotic effects, producing tissue factor, 16,17 providing a catalytic surface for thrombin generation, and forming platelet-leukocyte aggregates. 18 They may also exert vascular effects by compromising the microvasculature by adhesion, aggregation, and platelet recruitment 19 ; releasing proinflammatory and vasculotoxic factors 20 ; and contributing to intimal hyperplasia after vascular injury.…”

Section: Discussionmentioning

confidence: 99%

Preprocedural Levels of C-Reactive Protein and Leukocyte Counts Predict 9-Month Mortality After Coronary Angioplasty for the Treatment of Unprotected Left Main Coronary Artery Stenosis

et al. 2005

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Background-An accurate preprocedural risk stratification scheme for patients with unprotected left main coronary artery (ULMCA) stenosis who are undergoing coronary stenting is lacking. We examined the predictive value of preprocedural levels of C-reactive protein (CRP), fibrinogen, and leukocyte counts with respect to 9-month clinical outcomes after stenting of the ULMCA stenosis. Methods and Results-Levels of CRP, fibrinogen, and leukocyte count were prospectively measured in 83 patients undergoing stenting of the ULMCA. A drug-eluting stent was used in 42 patients, and a bare metal stent was used in 41. The end point of the study was death and the combination of death and myocardial infarction (MI). By the 9-month follow-up, there were 11 deaths (13%), 7 MIs (8%), and 16 target lesion revascularizations (19%). Death and death/MI occurred in 19% and 31%, respectively, of 59 patients with high serum levels of CRP (Ͼ3 mg/L) but in none of 24 patients with normal CRP levels (for death, Pϭ0.02; for death/MI, Pϭ0.006). In multivariate analysis, the highest tertiles of CRP level (Pϭ0.028) and leukocyte count (Pϭ0.002) were the only independent predictors of death. The highest tertiles of CRP level (Pϭ0.002) and leukocyte count (Pϭ0.002) and acute coronary syndromes (Pϭ0.05) were the only independent predictors of the combined end point death/MI. Conclusions-Elevated preprocedural levels of CRP indicate an increased risk of death and death/MI after ULMCA stenting. Inflammatory risk assessment in patients with ULMCA stenosis may be useful for selecting patients for percutaneous coronary interventions with very low risk.

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Monocyte-derived tissue factor contributes to stent thrombosis in an in vitro system

Abstract: Circulating TF plays a pivotal role in thrombus formation on stents. Monocytes appear to be the main, but not only, source of TF depositing in the thrombus.

Cited by 46 publications

References 28 publications

Co‐localization of von Willebrand factor with platelet thrombi, tissue factor and platelets with fibrin, and consistent presence of inflammatory cells in coronary thrombi obtained by an aspiration device from patients with acute myocardial infarction

Co‐localization of von Willebrand factor with platelet thrombi, tissue factor and platelets with fibrin, and consistent presence of inflammatory cells in coronary thrombi obtained by an aspiration device from patients with acute myocardial infarction

Impact of Leukocyte Count on Mortality and Bleeding in Patients With Myocardial Infarction Undergoing Primary Percutaneous Coronary Interventions

Preprocedural Levels of C-Reactive Protein and Leukocyte Counts Predict 9-Month Mortality After Coronary Angioplasty for the Treatment of Unprotected Left Main Coronary Artery Stenosis

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