Monocyte/macrophage functions in patients with squamous cell carcinoma of the oral cavity

Murali, Pazhayannur S.; Somasundaram, Rajasekharan; Chiplunkar, Shubhada V.; Fakih, Abdul R.; Rao, Renuka; Gangal, Sudha G.

doi:10.1111/j.1600-0714.1989.tb01550.x

Cited by 5 publications

(9 citation statements)

References 19 publications

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“…This assay was performed as described previously (Jacob et al ., 1997; Murali et al ., 1989). Briefly, lymphocytes (10 4 cultured T cells per well of 96‐well flat‐bottom microtiter plates; Corning) were stimulated with various numbers of tumor cells in the presence or absence of autologous EBV‐B cells and IL‐2 (partially purified, 20 U/ml) for 5–6 days.…”

Section: Methodsmentioning

confidence: 99%

CD4+, HLA class I-restricted, cytolytic T-lymphocyte clone against primary malignant melanoma cells

et al. 2000

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Cytolytic T cell (CTL) lines and clones have been established from various lymphoid sources of patients with metastatic melanoma, and the antigens (Ags) and peptides recognized by the CTL have been identified (reviewed by Lindauer et al., 1998). Most of the CTL directed against melanoma were CD8 ϩ and HLA-class I-restricted (frequently A1 or A2; reviewed by Lindauer et al., 1998). At least 14 different melanoma-associated CTL Ags and more than 26 peptides derived from these Ags have been described. These peptides were associated primarily with HLA-A2, followed by HLA-A1, -A24, -A31 and -Cw16 (reviewed by Lindauer et al., 1998), and sensitized HLA-matched target cells for CTL lysis. Peptides mimicking melanoma-associated Ags MAGE-1, MAGE-3, MART-1/MELAN-A, tyrosinase or gp100 induced proliferative and/or cytolytic T lymphocyte responses in melanoma patients (reviewed by Rivoltini et al., 1998). The clinical responses of the vaccinated patients were highly variable and need to be evaluated in randomized control studies.CD4 ϩ , HLA class II-restricted CTL against melanoma, have been described at a much lower frequency than CD8 ϩ , HLA class I-restricted CTL (Carrel and Johnson, 1993;de Vries and Spits, 1984;Thomas and Hersey, 1998). Such CD4 ϩ CTL usually showed low in vitro growth stabilities.A third type of CTL against melanoma that is of CD4 phenotype and may utilize HLA class I for tumor cell lysis has been described (Darrow et al., 1996;LeMay et al., 1993;Morisaki et al., 1994). Similar CTL have been found in colon and pancreatic carcinoma patients (Jacob et al., 1997). However, involvement of HLA class I in tumor cell lysis by the CD4 ϩ CTL has not been demonstrated unequivocally.We describe here a CD4 ϩ , HLA class I-restricted CTL clone that specifically and effectively lyses autologous melanoma cells. HLA-class I (B57 [17]) dependency of the CTL clone is demonstrated in CTL assays using various HLA-B57[17]-matched and -unmatched target cells and in CTL blocking assays with monoclonal antibody (MAb) to B17. Importantly, we demonstrate lysis of allogeneic HLA-class I-and II-unmatched melanoma cells by the CTL after transfection of the melanoma cells with HLA-B57 [17] cDNA. Furthermore, CD4 is involved in the lysis of autologous melanoma cells by the CTL. Thus, our results provides direct evidence for the involvement of both CD4 and HLA-class I in the CTL lysis of tumor cells. MATERIAL AND METHODS Patient 793Patient 793 (male Caucasian, 39 years of age) had excision of a ''low risk'' primary melanoma [superficial spreading type with early vertical growth phase present; the tumor thickness was 0.55 mm and the vertical growth phase had a brisk lymphatic infiltrate, with no evidence of metastases. The primary lesion was excised approx. 14 years earlier and there has been no recurrence since. Patient 793 did not receive any chemotherapy after the removal of primary lesion. Tumor cell linesMelanoma cell lines WM793, WM902B and WM3248 were established from the vertical growth phase of the primary lesions of mela...

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Section: Methodsmentioning

confidence: 99%

CD4+, HLA class I-restricted, cytolytic T-lymphocyte clone against primary malignant melanoma cells

et al. 2000

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“…This assay was performed as described previously (Jacob et al, 1997;Murali et al, 1989) All determinations were performed in triplicates with standard errors of the means of Ͻ 5%.…”

Section: T-cell Proliferation Assaymentioning

confidence: 99%

CD4+, HLA class I-restricted, cytolytic T-lymphocyte clone against primary malignant melanoma cells

et al. 2000

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“…and interferon-gamma (IFN-7) production. IL-2 was measured by assaying -^H-TdR incorporation by murine CTLL-2 IL-2-dependent T cells ( [21]; obtained from Dr W. Gerhard, The Wistar Institute; 1 x lO** cells/100//I of RPMI 1640 medium supplemented with 10% FCS per well of 96-weII microtitre plates) incubated with dilutions of T cell supematants for 24h. lL-4and IFN--> were measured by radioimmunoassay (RIA).…”

Section: Cytokine Assaysmentioning

confidence: 99%

“…Lymphocyte proliferation assays were performed as described earlier [21]. In brief, lymphocytes (1 x lO'/well of microtitre…”

Section: Prolifcrative Responses Of Hutnan Lymphocytes and Establishmmentioning

confidence: 99%

Tetanus toxoid-specific T cell responses in severe combined immunodeficiency (SCID) mice reconstituted with human peripheral blood lymphocytes

Somasundaram

Jacob

Herlyn

1995

Clinical and Experimental Immunology

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SUMMARY SCID mice reconstituted with human peripheral blood lymphocytes (PBL) have repeatedly been shown 10 produce antigen‐specific B ceil responses. We have derived tetanus toxoid (TT)‐specific human T cell lines from cells of the peritoneal cavity, spleen and lymph nodes of SCID mice reconstituted with human PBL and boosted with TT. Establishment of these cell lines was dependent on the time interval between reconstitution of the mice with human PBL and initiation of lymphocyte cultures in vitro. When lymphocytes were collected from the mice 8 weeks after reconstitution, human lymphocytes with TT‐specific proliferative activity in vitro were isolated from the peritoneal cavity and spleen, but long‐term cell lines could not be established after repeated lymphocyte stimulation with TT. IL‐2 and autologous Epstein Barr virus‐transformed B cells. In contrast, three long‐term (>10 months) TT‐specific human T cell lines were established from lymphocytes collected from two of the eight mice in the group 4 weeks after reconstitution. The T cell lines were either CD4+ (two lines derived from peritoneal cavity and lymph node, respectively) or CD8+ (one line derived from spleen) and all expressed CD3, T cell receptor α/β, and human histocompatibility leucocyte class I antigen. The T cell lines, however, lacked cytotoxic, helper and suppressor activities. Thus, SCID mice can support human T cells that actively migrate to various organs and respond to antigenic stimuli both in vivo and in vitro, but these T cells lack characteristic functions.

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Monocyte/macrophage functions in patients with squamous cell carcinoma of the oral cavity

Cited by 5 publications

References 19 publications

CD4+, HLA class I-restricted, cytolytic T-lymphocyte clone against primary malignant melanoma cells

CD4+, HLA class I-restricted, cytolytic T-lymphocyte clone against primary malignant melanoma cells

CD4+, HLA class I-restricted, cytolytic T-lymphocyte clone against primary malignant melanoma cells

Tetanus toxoid-specific T cell responses in severe combined immunodeficiency (SCID) mice reconstituted with human peripheral blood lymphocytes

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