Monocyte profile in peripheral blood of gestational diabetes mellitus patients

Angelo, Ana G.S.; Neves, Carla T.C.; Lobo, Thalita Frutuoso; Godoy, R.V.C.; Ono, Érika; Mattar, Rosiane; Daher, Sílvia

doi:10.1016/j.cyto.2017.11.017

Cited by 21 publications

(26 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The same observations had been made in women with GDM in whom the profiles of CD3+, CD4+, and CD8+ T and Treg cells were similar to those observed in insulin-treated pregnant women with T2D, with the exception of B cells which increased in GDM compared to pregnant women with T2D and control pregnant women. In fact, our results were supported by those of Angelo et al [20] and Mahmoud et al [48] who have also observed high percentages of CD4+CD25+ and CD4+Th17 cells in insulin-treated women with GDM, suggesting that insulin treatment did not influence the proportion of these cells in pregnant women with in type 2 diabetes as well as in women with GDM [49].…”

Section: Discussionsupporting

confidence: 90%

“…Pregnant women with preexisting insulin-treated T2D were separately selected in the population of women already monitored by the clinicians at the department of obstetrics and gynecology of these hospital centers. The number of twenty (20) insulin-treated T2D pregnant women corresponds to the mean of the number of women with GDM (15) and the number of control pregnant (25) women. The size of each group, fifteen (15) women with GDM, twenty (20) pregnant women with insulin-treated T2D, and twenty-five (25) pregnant controls, was appropriate for statistics.…”

Section: Subjects and Diabetes Diagnosis In Pregnant Womenmentioning

confidence: 99%

“…The number of twenty (20) insulin-treated T2D pregnant women corresponds to the mean of the number of women with GDM (15) and the number of control pregnant (25) women. The size of each group, fifteen (15) women with GDM, twenty (20) pregnant women with insulin-treated T2D, and twenty-five (25) pregnant controls, was appropriate for statistics. All selected participants were then submitted to blood collection for biochemical and immunological assays.…”

Section: Subjects and Diabetes Diagnosis In Pregnant Womenmentioning

confidence: 99%

“…Nonetheless, some studies showed that GDM is not only due to metabolic disturbances during pregnancy but also by a state of low-grade systemic inflammation [18]. This dysregulation of the immune system is characterized by an altered profile of monocytes and regulatory T cells in peripheral blood and an imbalance between T helper 1 (Th1) and 2 (Th2) cells favoring proinflammatory responses [12,[19][20][21]. As the hyperglycemia and disrupted immunological adaptations during pregnancy are responsible for increased maternal-fetal morbidity, as well as the short-and longterm complications in mothers and offspring [1,22,23], improving glycemic control by insulin therapy may substantially reduce the risks of these maternal and neonatal outcomes of diabetes in pregnancy [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Th2-Immune Polarizing and Anti-Inflammatory Properties of Insulin Are Not Effective in Type 2 Diabetic Pregnancy

Fagninou

Nekoua

Sossou³

et al. 2020

Journal of Immunology Research

View full text Add to dashboard Cite

Background. The implication of the immune system in the physiopathology of pregnancy complicated by diabetes has been reported. Here, we investigated the effects of insulin treatment on the frequencies of immune cell subpopulations as well as T cell-derived cytokines in type 2 diabetic (T2D) pregnancy compared to gestational diabetes mellitus (GDM). Methods. Fifteen (15) women with GDM, twenty (20) insulin-treated T2D pregnant women, and twenty-five (25) pregnant controls were selected. Immune cell subpopulation frequencies were determined in blood using flow cytometry. The proliferative capacity of T cells was performed, and serum and cell culture supernatant cytokine levels were also quantified. Results. The frequencies of total CD3+ and CD4+ T cells and nonclassical monocytes significantly increased in insulin-treated T2D pregnant women compared to pregnant controls. The proportions of CD4+ T cells as well as B cells were significantly higher in women with GDM than in pregnant controls. GDM was associated with high frequencies of total CD3+ and CD4+ T cells and B cell expansion, suggesting a concomitant activation of cellular and humoral immunity. Concomitantly, Th1/Th2 ratio, determined as IFN-γ/IL-4, was shifted towards Th1 phenotype in women with GDM and insulin-treated T2D pregnant women. Besides, isolated T cells elicited similar proliferative capacity in the three groups of women. Insulin-treated T2D pregnant women and women with GDM exhibited a low serum IL-10 level, without any change in the number of Treg cells. Conclusion. Our study showed that, despite insulin treatment, pregnant women with T2D displayed a proinflammatory status consistent with high proportions of CD3+ and CD4+ T cells, upregulation of Th1 cytokines, and low IL-10 production, suggesting a reduced immune-suppressive activity of regulatory T cells. However, GDM, although associated with proinflammatory status, has shown increased humoral immunity consistent with high proportion of CD19+ B cells. Thus, the lack of response to insulin in diabetes during pregnancy and clinical implications of these immunological parameters deserves further investigations.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Subjects and Diabetes Diagnosis In Pregnant Womenmentioning

confidence: 99%

Section: Subjects and Diabetes Diagnosis In Pregnant Womenmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Th2-Immune Polarizing and Anti-Inflammatory Properties of Insulin Are Not Effective in Type 2 Diabetic Pregnancy

Fagninou

Nekoua

Sossou³

et al. 2020

Journal of Immunology Research

View full text Add to dashboard Cite

show abstract

“…16,54 Only one retrieved study analysed this population in GDM. Angelo et al 55 observed increased numbers of monocytes (total) and lower percentage of intermediate subset in the diabetic compared to controls. Likewise, this small subpopulation seems to exert an immunomodulatory effect, so its decrease in GDM may contribute to the development of inflammation.…”

Section: Monocytesmentioning

confidence: 95%

Unveiling the pathophysiology of gestational diabetes: Studies on local and peripheral immune cells

et al. 2020

Self Cite

View full text Add to dashboard Cite

Gestational diabetes mellitus (GDM) has been associated with impaired maternal immune response. Our aim was to review the available literature linking immune cells profile to GDM, in order to comprehend the role that different subpopulations play in the development of this pathology. We searched in PubMed for studies published in the last decade on circulating levels and placenta expression of immune cells on GDM. We identified 18 studies with several differences regarding the study design, clinical characteristics, number of participants, cell subpopulation and type of sample. Most studies assessed only one subpopulation either in peripheral blood or placenta and did not analyse functional properties of the cells. The most frequently evaluated immune cells were T lymphocytes, especially regulatory T (Tregs), and natural killer (NK) cells in the peripheral blood, and placental macrophages. No studies analysing B cells were identified, and only one study each evaluating γδT cells, dendritic cell (DC) and monocytes was found. Although there are controversies, at least one study reported positive association between GDM and CD4+(activated), Tregs, Th17 and γδT cells; neutrophil/lymphocyte; NK cell (cytotoxic); macrophages; and monocytes. The number of studies is still small, so caution should be exercised in interpreting the data, and further research is required to validate these findings and establish the role of adaptive and innate immune cells in GDM pathophysiology.

show abstract

The birth of cardiac disease: Mechanisms linking gestational diabetes mellitus and early onset of cardiovascular disease in offspring

Tocantins

Diniz

Grilo

et al. 2022

WIREs Mechanisms of Disease

View full text Add to dashboard Cite

Cardiovascular disease (CVD) is the biggest killer worldwide, composing a major economic burden for health care systems. Obesity and diabetes are dual epidemics on the rise and major risk factors predisposing for CVD. Increased obesity‐ and diabetes‐related incidence is now observed among children, adolescents, and young adults. Gestational diabetes mellitus (GDM) is the most common metabolic pregnancy disorder, and its prevalence is rapidly increasing. During pregnancies complicated by GDM, the offspring are exposed to a compromised intrauterine environment characterized by hyperglycemic periods. Unfavorable in utero conditions at critical periods of fetal cardiac development can produce developmental adaptations that remodel the cardiovascular system in a way that can contribute to adult‐onset of heart disease due to the programming during fetal life. Epidemiological studies have reported increased cardiovascular complications among GDM‐descendants, highlighting the urgent need to investigate and understand the mechanisms modulated during fetal development of in utero GDM‐exposed offspring that predispose an individual to increased CVD during life. In this manuscript, we overview previous studies in this area and gather evidence linking GDM and CVD development in the offspring, providing new insights on novel mechanisms contributing to offspring CVD programming by GDM, from the role of maternal–fetal interactions to their impact on fetal cardiovascular development, how the perpetuation of cardiac programming is maintained in postnatal life, and advance the intergenerational implications contributing to increased CVD premature origin. Understanding the perpetuation of CVD can be the first step to manage and reverse this leading cause of morbidity and mortality. This article is categorized under: Reproductive System Diseases > Molecular and Cellular Physiology Cardiovascular Diseases > Molecular and Cellular Physiology Metabolic Diseases > Genetics/Genomics/Epigenetics

show abstract

Monocyte profile in peripheral blood of gestational diabetes mellitus patients

Cited by 21 publications

References 32 publications

Th2-Immune Polarizing and Anti-Inflammatory Properties of Insulin Are Not Effective in Type 2 Diabetic Pregnancy

Th2-Immune Polarizing and Anti-Inflammatory Properties of Insulin Are Not Effective in Type 2 Diabetic Pregnancy

Unveiling the pathophysiology of gestational diabetes: Studies on local and peripheral immune cells

The birth of cardiac disease: Mechanisms linking gestational diabetes mellitus and early onset of cardiovascular disease in offspring

Contact Info

Product

Resources

About