Monocytes and macrophages in flow cytometry

Lambert, Claude; Sack, Ulrich

doi:10.1002/cyto.b.21530

Cited by 8 publications

(5 citation statements)

References 10 publications

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“…In cirrhotic patients, [130], Cardoso et al observed an increase in circulating intermediate monocytes (CD14 + CD16 + ), distinguished from the classical monocytes (CD14 ++ CD16 − ) and from nonclassical monocytes (CD14 −/low CD16 + ) [131]. They detected alterations in the proportions of circulating monocytes, particularly in patients with more advanced liver disease.…”

Section: Bone-marrow/monocyte-derived Macrophagesmentioning

confidence: 97%

Cellular and Molecular Mechanisms Underlying Liver Fibrosis Regression

Caligiuri

Gentilini

Pastore

et al. 2021

Cells

137

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Chronic liver injury of different etiologies may result in hepatic fibrosis, a scar formation process consisting in altered deposition of extracellular matrix. Progression of fibrosis can lead to impaired liver architecture and function, resulting in cirrhosis and organ failure. Although fibrosis was previous thought to be an irreversible process, recent evidence convincingly demonstrated resolution of fibrosis in different organs when the cause of injury is removed. In the liver, due to its high regenerative ability, the extent of fibrosis regression and reversion to normal architecture is higher than in other tissues, even in advanced disease. The mechanisms of liver fibrosis resolution can be recapitulated in the following main points: removal of injurious factors causing chronic hepatic damage, elimination, or inactivation of myofibroblasts (through various cell fates, including apoptosis, senescence, and reprogramming), inactivation of inflammatory response and induction of anti-inflammatory/restorative pathways, and degradation of extracellular matrix. In this review, we will discuss the major cellular and molecular mechanisms underlying the regression of fibrosis/cirrhosis and the potential therapeutic approaches aimed at reversing the fibrogenic process.

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Section: Bone-marrow/monocyte-derived Macrophagesmentioning

confidence: 97%

Cellular and Molecular Mechanisms Underlying Liver Fibrosis Regression

Caligiuri

Gentilini

Pastore

et al. 2021

Cells

137

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“…Circulating monocytes have both pro‐inflammatory and resolution functions and are subdivided into three types: classical monocytes (cMo, CD14 ++ CD16 − ), intermediate monocytes (iMos, CD14 + CD16 + ), and nonclassical monocytes (ncMo, CD14 −/low CD16 + ). iMo and ncMo are also called tissue macrophages (TiMas) [ 16 ]. In turn, DCs are potent APCs, leading to naive T‐cell activation and effector differentiation [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

The relationship between peripheral immune response and disease severity in SARS‐CoV‐2‐infected subjects: A cross‐sectional study

et al. 2022

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Coronavirus disease 2019 (COVID-19) is a respiratory infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and marked by an intense inflammatory response and immune dysregulation in the most severe cases. In order to better clarify the relationship between peripheral immune system changes and the severity of COVID-19, this study aimed to evaluate the frequencies and absolute numbers of peripheral subsets of neutrophils, monocytes, and dendritic cells (DCs), in addition to quantifying the levels of inflammatory mediators. One hundred fifty-seven COVID-19 patients were stratified into mild, moderate, severe, and critical disease categories. The cellular components and circulating cytokines were assessed by flow cytometry. Nitric oxide (NOx) and myeloperoxidase (MPO) levels were measured by colourimetric tests. COVID-19 patients presented neutrophilia, with signs of emergency myelopoiesis. Alterations in the monocytic component were observed in patients with moderate to critical illness, with an increase in classical monocytes and a reduction in nonclassical monocytes, in addition to a reduction in the expression of HLA-DR in all subtypes of monocytes, indicating immunosuppression. DCs, especially plasmacytoid DCs, also showed a large reduction in moderate to critical patients. COVID-19 patients showed an increase in MPO, interleukin (IL)-12, IL-6, IL-10, and IL-8, accompanied by a reduction in IL-17A and NOx. IL-10 levels ≥14 pg/ml were strongly related to the worst outcome, with a sensitivity of 78•3% and a specificity of 79•1%. The results of this study indicate the presence of systemic

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“…[1,2] It has the advantages of simplicity, rapidity, many cell counts and high accuracy. [3] In recent years, with the popularization of flow cytometry, flow cytometry technology has attracted more and more clinical attention, especially lymphocyte subsets is the most common FCM test item in clinic. [4,5] Lymphocytes are a very important group of cells in the immune system.…”

Section: Introductionmentioning

confidence: 99%

Comparison of different combinations of antibodies and labeled fluorescein in the detection of lymphocyte subsets by flow cytometry

Ban

Zhao

2022

Medicine

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Flow cytometry is a classical method for analyzing human peripheral blood lymphocyte subsets. This study aims to explore a new combination of antibody and labeled fluorescein for detecting lymphocyte subsets by comparing the effects of different combinations of antibody and labeled fluorescein in flow cytometry. We conducted a prospective study and enrolled 362 healthy patients undergoing physical examination in the medical examination center of the third hospital of hebei medical university. Venous blood was drawn from volunteers at the same time in the morning and divided into 3 tubes (Tube A, Tube B and Tube C). T lymphocytes were detected by 3-colors method (CD4-FITC/CD8-PE/CD3-PC5) in Tube A, B lymphocytes were detected by 2-colors method (CD19-FITC/CD3-PE) in Tube B, and T lymphocytes and B lymphocytes were detected by 4-colors method (CD4-FITC/CD8-PE/CD3-PC5/CD19-FITC) in Tube C. The repeatability and accuracy of the test scheme for Tube C shall not be inferior to that of Tube A and Tube B. There were no significant difference in the results of CD3 + and CD4+/CD8 + between Tube A and C, as well as in the results of CD3 + and CD19 + between Tube B and C. Pearson correlation analysis showed that the test results of a and C and B and C were highly correlated. The 4-colors method (CD4-FITC/CD8-PE/CD3-PC5/CD19-FITC) can detect T lymphocytes and B lymphocytes at the same time, reduce the use of fluorescence channels and save the detection cost, which is worthy of recommendation.

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Monocytes and macrophages in flow cytometry

Cited by 8 publications

References 10 publications

Cellular and Molecular Mechanisms Underlying Liver Fibrosis Regression

Cellular and Molecular Mechanisms Underlying Liver Fibrosis Regression

The relationship between peripheral immune response and disease severity in SARS‐CoV‐2‐infected subjects: A cross‐sectional study

Comparison of different combinations of antibodies and labeled fluorescein in the detection of lymphocyte subsets by flow cytometry

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