Monodisperse red blood cell-like particles via consolidation of charged droplets

Park, Chul Ho; Chung, Nae-oh; Lee, Jonghwi

doi:10.1016/j.jcis.2011.06.003

Cited by 25 publications

(18 citation statements)

References 43 publications

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“…Although the high throughput production of RBC templates that fulfill all these requirements continues to be a big challenge, in recent years, there have been important advances. In this section, it should be pointed out that we will focus our attention on particles with at least a similar size to that of RBCs, despite the fact that a few works can be found in the literature fabricating/using particles with sizes smaller than 5 μm [ 29 , 30 , 31 , 32 ].…”

Section: Rbc Templatesmentioning

confidence: 99%

Blood Particulate Analogue Fluids: A Review

et al. 2021

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Microfluidics has proven to be an extraordinary working platform to mimic and study blood flow phenomena and the dynamics of components of the human microcirculatory system. However, the use of real blood increases the complexity to perform these kinds of in vitro blood experiments due to diverse problems such as coagulation, sample storage, and handling problems. For this reason, interest in the development of fluids with rheological properties similar to those of real blood has grown over the last years. The inclusion of microparticles in blood analogue fluids is essential to reproduce multiphase effects taking place in a microcirculatory system, such as the cell-free layer (CFL) and Fähraeus–Lindqvist effect. In this review, we summarize the progress made in the last twenty years. Size, shape, mechanical properties, and even biological functionalities of microparticles produced/used to mimic red blood cells (RBCs) are critically exposed and analyzed. The methods developed to fabricate these RBC templates are also shown. The dynamic flow/rheology of blood particulate analogue fluids proposed in the literature (with different particle concentrations, in most of the cases, relatively low) is shown and discussed in-depth. Although there have been many advances, the development of a reliable blood particulate analogue fluid, with around 45% by volume of microparticles, continues to be a big challenge.

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Section: Rbc Templatesmentioning

confidence: 99%

Blood Particulate Analogue Fluids: A Review

et al. 2021

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“…Sun et al (2011) fabricated quantum dot (CdSeS QDs)-encoded poly(styrene-acrylate) microspheres by electrospraying the mixture of polymer solution and quantum dots solution, providing many reliable codes for multiplex detection. In different studies, Park et al (2011, 2012b, 2013) demonstrated the fabrication of PVA/MMT, PVA/Ag, and PVA/MMT/Ag composite particles using electrospray and explored the test of antibacterial activity against Staphylococcus aureus . In addition, a more recent study demonstrated the fabrication of micellar (polystyrene-block-ethylene oxide) nanocomposites containing quantum dots (QDs), superparamagnetic iron oxide nanoparticles (SPIONs) and their combination via co-axial electrospray (Duong et al, 2014).…”

Section: Compositionmentioning

confidence: 99%

Electrohydrodynamic atomization: A two-decade effort to produce and process micro-/nanoparticulate materials

Xie

Jiang

Davoodi

et al. 2015

Chemical Engineering Science

268

159

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Electrohydrodynamic atomization (EHDA), also called electrospray technique, has been studied for more than one century. However, since 1990s it has begun to be used to produce and process micro-/nanostructured materials. Owing to the simplicity and flexibility in EHDA experimental setup, it has been successfully employed to generate particulate materials with controllable compositions, structures, sizes, morphologies, and shapes. EHDA has also been used to deposit micro- and nanoparticulate materials on surfaces in a well-controlled manner. All these attributes make EHDA a fascinating tool for preparing and assembling a wide range of micro- and nanostructured materials which have been exploited for use in pharmaceutics, food, and healthcare to name a few. Our goal is to review this field, which allows scientists and engineers to learn about the EHDA technique and how it might be used to create, process, and assemble micro-/nanoparticulate materials with unique and intriguing properties. We begin with a brief introduction to the mechanism and setup of EHDA technique. We then discuss issues critical to successful application of EHDA technique, including control of composition, size, shape, morphology, structure of particulate materials and their assembly. We also illustrate a few of the many potential applications of particulate materials, especially in the area of drug delivery and regenerative medicine. Next, we review the simulation and modeling of Taylor cone-jet formation for a single and co-axial nozzle. The mathematical modeling of particle transport and deposition is presented to provide a deeper understanding of the effective parameters in the preparation, collection and pattering processes. We conclude this article with a discussion on perspectives and future possibilities in this field.

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“…This, thus, requires sacrificial discoid templates or lithographically templated substrates to fabricate concave particles, as described in previous reports, which makes the process more complex and expensive [ 23 , 24 ]. To overcome the deficiencies of the template-based approaches, coaxial electrosprays adopting two different fluids have been developed for anisotropically structuring droplets during the spraying to produce concave polymers and lipid particles [ 25 , 26 ]. However, this electrospray approach is not valid for fabricating NPs (only valid for microparticles) because of a short mutual diffusion distance between the small droplets [ 25 ], although smaller particles can exhibit better activities in intracellular penetration and microvascular circulation to reach distant tissues even in biomimetic delivery [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

“…To overcome the deficiencies of the template-based approaches, coaxial electrosprays adopting two different fluids have been developed for anisotropically structuring droplets during the spraying to produce concave polymers and lipid particles [ 25 , 26 ]. However, this electrospray approach is not valid for fabricating NPs (only valid for microparticles) because of a short mutual diffusion distance between the small droplets [ 25 ], although smaller particles can exhibit better activities in intracellular penetration and microvascular circulation to reach distant tissues even in biomimetic delivery [ 27 ]. Although the sizes of the concave particles produced by the coaxial electrosprays are similar to those of red blood cells (6–8 μm) [ 24 ], no studies on the efficacy for anticancer applications have been performed.…”

Section: Introductionmentioning

confidence: 99%

Artificial Nanoscale Erythrocytes from Clinically Relevant Compounds for Enhancing Cancer Immunotherapy

Nam

Park

et al. 2020

Nano-Micro Lett.

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ARTICLE HIGHLIGHTS• A two-phase coaxial electrospray was designed to produce paclitaxel-loaded fake blood cell Eudragit particles (Eu-FBCP/PTX).• The chemo-immunotherapeutic efficacy was further enhanced after combining with anti-programmed death-ligand 1 antibodies (Eu-FBCP/PTX + aPL).ABSTRACT Because of enhanced efficacy and lower side effects, cancer immunotherapies have recently been extensively investigated in clinical trials to overcome the limitations of conventional cancer monotherapies. Although engineering attempts have been made to build nanosystems even including stimulus nanomaterials for the efficient delivery of antigens, adjuvants, or anticancer drugs to improve immunogenic cancer cell death, this requires huge R&D efforts and investment for clinically relevant findings to be approved for translation of the nanosystems. To this end, in this study, an air-liquid two-phase electrospray was developed for stable bubble pressing under a balance between mechanical and electrical parameters of the spray to continuously produce biomimetic nanosystems consisting of only clinically relevant compounds [paclitaxel-loaded fake blood cell Eudragit particle (Eu-FBCP/PTX)] to provide a conceptual leap for the timely development of translatable chemo-immunotherapeutic nanosystems. This was pursued as the efficacy of systems for delivering anticancer agents that has been mainly influenced by nanosystem shape because of its relevance to transporting behavior to organs, blood circulation, and cell-membrane interactions. The resulting Eu-FBCP/PTX nanosystems exhibiting phagocytic and micropinocytic uptake behaviors can confer better efficacy in chemo-immunotherapeutics in the absence and presence of anti-PD-L1 antibodies than similar sized PTX-loaded spherical Eu particles (Eu-s/PTX).

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Monodisperse red blood cell-like particles via consolidation of charged droplets

Cited by 25 publications

References 43 publications

Blood Particulate Analogue Fluids: A Review

Blood Particulate Analogue Fluids: A Review

Electrohydrodynamic atomization: A two-decade effort to produce and process micro-/nanoparticulate materials

Artificial Nanoscale Erythrocytes from Clinically Relevant Compounds for Enhancing Cancer Immunotherapy

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