Monolayer grafting of aminosilane on magnetic nanoparticles: An efficient approach for targeted drug delivery system

Dhavale, Rakesh P.; Waifalkar, P. P.; Sharma, Apoorva; Sahoo, Subasa C.; Kollu, Pratap; Chougale, Ashok D.; Zahn, Dietrich R. T.; Salvan, Georgeta; Patil, Pramod

doi:10.1016/j.jcis.2018.06.006

Cited by 61 publications

(17 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, the spectrum in Fig. 2b may contain a stretching vibration of Fe-O-Si at 589 cm −1 overlaying with Fe-O vibrations from iron oxide 50 . The peak corresponding to Si-O-Si at 1015 cm −1 , and the bending and stretching vibrational modes of NH at 1637 cm −1 and 3400 cm −1 , respectively, confirm the coverage of silane with a free amine group of APTES on the MNS surface 58,59 .…”

Section: Resultsmentioning

confidence: 99%

APTES monolayer coverage on self-assembled magnetic nanospheres for controlled release of anticancer drug Nintedanib

Karade

Sharma

Dhavale

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

The use of an appropriate delivery system capable of protecting, translocating, and selectively releasing therapeutic moieties to desired sites can promote the efficacy of an active compound. In this work, we have developed a nanoformulation which preserves its magnetization to load a model anticancerous drug and to explore the controlled release of the drug in a cancerous environment. For the preparation of the nanoformulation, self-assembled magnetic nanospheres (MNS) made of superparamagnetic iron oxide nanoparticles were grafted with a monolayer of (3-aminopropyl)triethoxysilane (APTES). A direct functionalization strategy was used to avoid the loss of the MNS magnetization. The successful preparation of the nanoformulation was validated by structural, microstructural, and magnetic investigations. X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR) were used to establish the presence of APTES on the MNS surface. The amine content quantified by a ninhydrin assay revealed the monolayer coverage of APTES over MNS. The monolayer coverage of APTES reduced only negligibly the saturation magnetization from 77 emu/g (for MNS) to 74 emu/g (for MNS-APTES). Detailed investigations of the thermoremanent magnetization were carried out to assess the superparamagnetism in the MNS. To make the nanoformulation pH-responsive, the anticancerous drug Nintedanib (NTD) was conjugated with MNS-APTES through the acid liable imine bond. At pH 5.5, which mimics a cancerous environment, a controlled release of 85% in 48 h was observed. On the other hand, prolonged release of NTD was found at physiological conditions (i.e., pH 7.4). In vitro cytotoxicity study showed dose-dependent activity of MNS-APTES-NTD for human lung cancer cells L-132. About 75% reduction in cellular viability for a 100 μg/mL concentration of nanoformulation was observed. The nanoformulation designed using MNS and monolayer coverage of APTES has potential in cancer therapy as well as in other nanobiological applications.

show abstract

Section: Resultsmentioning

confidence: 99%

APTES monolayer coverage on self-assembled magnetic nanospheres for controlled release of anticancer drug Nintedanib

Karade

Sharma

Dhavale

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…For magnetic targeting, magnetic nanoparticles based on iron oxides should possess some key parameters, including: (i) high surface area to maximize the drug-loading amount, (ii) nanoparticle surface functionalization with specific targeting moieties to facilitate drug distribution at the tumor site while avoiding damage to other organs, (iii) a high saturation field to provide the maximum number of signals, and (iv) synergistic activity with antitumor agents to improve the efficacy of cancer treatment by decreasing the resistance of cancerous cells [ 16 , 50 , 51 , 52 ]. For drug delivery, it is imperative that the magnetic core of the nanomaterial (usually magnetite, Fe 3 O 4 , or maghemite, γ-Fe 2 O 3 ) to be nanocrystalline and to possess superparamagnetic behavior only in the presence of an external magnetic field, and outside of its presence the nanoparticles should no longer exhibit magnetic interactions.…”

Section: Discussionmentioning

confidence: 99%

Biocompatible Magnetic Colloidal Suspension Used as a Tool for Localized Hyperthermia in Human Breast Adenocarcinoma Cells: Physicochemical Analysis and Complex In Vitro Biological Profile

et al. 2021

View full text Add to dashboard Cite

Magnetic iron oxide nanoparticles are the most desired nanomaterials for biomedical applications due to their unique physiochemical properties. A facile single-step process for the preparation of a highly stable and biocompatible magnetic colloidal suspension based on citric-acid-coated magnetic iron oxide nanoparticles used as an effective heating source for the hyperthermia treatment of cancer cells is presented. The physicochemical analysis revealed that the magnetic colloidal suspension had a z-average diameter of 72.7 nm at 25 °C with a polydispersity index of 0.179 and a zeta potential of −45.0 mV, superparamagnetic features, and a heating capacity that was quantified by an intrinsic loss power analysis. Raman spectroscopy showed the presence of magnetite and confirmed the presence of citric acid on the surfaces of the magnetic iron oxide nanoparticles. The biological results showed that breast adenocarcinoma cells (MDA-MB-231) were significantly affected after exposure to the magnetic colloidal suspension with a concentration of 30 µg/mL 24 h post-treatment under hyperthermic conditions, while the nontumorigenic (MCF-10A) cells exhibited a viability above 90% under the same thermal setup. Thus, the biological data obtained in the present study clearly endorse the need for further investigations to establish the clinical biological potential of synthesized magnetic colloidal suspension for magnetically triggered hyperthermia.

show abstract

“…The FTIR spectra demonstrate the absorption bands for the silanol (Si-O-H) and siloxane (Si-O-Si) groups. The band 1008 cm −1 of Fe3O4/APTES (air) is attributed to the Si-O-Si groups, respectively [36,38], confirming the adsorption of APTES on the MNP surface. The band around 992 cm −1 of Fe3O4-APTES(Ar) is assigned to the Si-O [39] or Si-OH [38] groups.…”

Section: Microstructure Of Fe3o4/aptes Mnpsmentioning

confidence: 70%

“…The band 1008 cm −1 of Fe3O4/APTES (air) is attributed to the Si-O-Si groups, respectively [36,38], confirming the adsorption of APTES on the MNP surface. The band around 992 cm −1 of Fe3O4-APTES(Ar) is assigned to the Si-O [39] or Si-OH [38] groups. At the same time, White [40] reported that bands 1008 or 992 cm −1 could be attributed to the SiO bond of the SiOH-group, which H-bonded to NH2-group (Figure 3, case 4).…”

Section: Microstructure Of Fe3o4/aptes Mnpsmentioning

confidence: 70%

Fabrication, Microstructure and Colloidal Stability of Humic Acids Loaded Fe3O4/APTES Nanosorbents for Environmental Applications

et al. 2021

View full text Add to dashboard Cite

Nowadays, numerous researches are being performed to formulate nontoxic multifunctional magnetic materials possessing both high colloidal stability and magnetization, but there is a demand in the prediction of chemical and colloidal stability in water solutions. Herein, a series of silica-coated magnetite nanoparticles (MNPs) has been synthesized via the sol-gel method with and without establishing an inert atmosphere, and then it was tested in terms of humic acids (HA) loading applied as a multifunctional coating agent. The influence of ambient conditions on the microstructure, colloidal stability and HA loading of different silica-coated MNPs has been established. The XRD patterns show that the content of stoichiometric Fe3O4 decreases from 78.8% to 42.4% at inert and ambient atmosphere synthesis, respectively. The most striking observation was the shift of the MNPs isoelectric point from pH~ 7 to 3, with an increasing HA reaching up to the reversal of the zeta potential sign as it was covered completely by HA molecules. The zeta potential data of MNPs can be used to predict the loading capacity for HA polyanions. The data help to understand the way for materials’ development with the complexation ability of humic acids and with the insolubility of silica gel to pave the way to develop a novel, efficient and magnetically separable adsorbent for contaminant removal.

show abstract

Monolayer grafting of aminosilane on magnetic nanoparticles: An efficient approach for targeted drug delivery system

Cited by 61 publications

References 91 publications

APTES monolayer coverage on self-assembled magnetic nanospheres for controlled release of anticancer drug Nintedanib

APTES monolayer coverage on self-assembled magnetic nanospheres for controlled release of anticancer drug Nintedanib

Biocompatible Magnetic Colloidal Suspension Used as a Tool for Localized Hyperthermia in Human Breast Adenocarcinoma Cells: Physicochemical Analysis and Complex In Vitro Biological Profile

Fabrication, Microstructure and Colloidal Stability of Humic Acids Loaded Fe3O4/APTES Nanosorbents for Environmental Applications

Contact Info

Product

Resources

About