Monolayer interactions of individual lecithins with natural sterols

Ghosh, Dolly; Tinoco, J.

doi:10.1016/0005-2736(72)90117-4

Cited by 83 publications

(38 citation statements)

References 10 publications

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“…Model system studies have shown that the type and ratio of phospholipids and sterol incorporated in a bilayer can have significant effects on the properties of the bilayer. In particular, it has been shown that increased ratios of sterol to phospholipid inhibit mobility (Darke et al, 1972), that ergosterol is less effective than cholesterol in condensing phospholipid bilayers (Butler, Smith & Schneider, 1970;Demel Bruckdorfer & van Deenen, 1972;Ghosh & Tinoco, 1972;Hsia, Long, Hruska & Gesser, 1972), and that increased proportions of unsaturated fatty acids increase bilayer fluidity (Chapman & Leslie, 1971). For the last two reasons, lipid bilayers in yeast mitochondria would be expected to be more fluid and hence exhibit a more resolved NMR spectrum than similar regions in rat liver mitochondria.…”

Section: Discussionmentioning

confidence: 98%

Comparison of membrane organization in mitochondria from yeast and rat liver by nuclear magnetic resonance spectroscopy

et al. 1975

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Proton magnetic resonance (PMR) and carbon-13 magnetic resonance (CMR) spectra of intact, unsonicated yeast and rat liver motochondria show differences which may be correlated with the composition of the membranes. High resolution PMR and CMR signals in intact yeast mitochondria have been assigned to regions of fluid lipid-lipid interaction on the basis of spectra of extracted lipid and protein, and the temperature dependence of NMR signals from the intact membrane. PMR spectra suggest that about 20% of total yeast phospholipid is in regions where both intramolecular fatty acid chain mobility and lateral diffusion of entire phospholipid molecules are possible. No such regions apear to exist in rat liver mitochondria. For both yeast and rat liver mitochondria, comparison of PMR and CMR spectra suggests that about 50% of phospholipid appears to be in regions where intramolecular fatty acid chain motion is considerable, but lateral diffusion is restricted. The remaining phospholipid appears to have little inter- or intramolecular mobility. Since NMR observation of lipid extracts from membranes indicates that phospholipid-sterol interactions do not account for the spectra of intact mitochondria, these effects are interpreted in terms of extensive lipid-protein interactions.

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Section: Discussionmentioning

confidence: 98%

Comparison of membrane organization in mitochondria from yeast and rat liver by nuclear magnetic resonance spectroscopy

et al. 1975

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“…A wide variety of experimental methods indicate, however, that cholesterol exhibits a preferential affinity for saturated over polyunsaturated chains and is incompatible with DHA. The sterol condenses monolayers made from phospholipids containing a saturated chain in the sn-1 position and a saturated, oleic (18:1) or ␣-linolenic (␣18:3) chain, but not DHA, in the sn-2 position (Demel et al, 1972;Ghosh and Tinoco, 1972). Contrary to most fatty acids, cholesterol has little effect on the permeability of DHA-containing membranes (Stillwell et al, 1996).…”

Section: Dha-cholesterol Interactionsmentioning

confidence: 98%

Order from disorder, corralling cholesterol with chaotic lipids

Wassall

Brzustowicz

Shaikh

et al. 2004

Chemistry and Physics of Lipids

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“…Many studies have demonstrated that sterols greatly affect the barrier properties of membranes. Monolayer studies have demonstrated a so-called condensing effect [1][2][3], this is a decrease in mean molecular area in mixtures of phospholipid and cholesterol. The increase in chain order in the liquid-crystalline state leads to a decreased permeability [4,5].…”

Section: Introductionmentioning

confidence: 99%

The effect of the sterol oxygen function on the interaction with phospholipids

Demel

Lala

Kumari

et al. 1984

Biochimica et Biophysica Acta (BBA) - Biomembranes

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The effect of cholesteryi ethers (namely cholesteryl methyl ether, cholesteryi ethyl ether, cholesteryl n-propyl ether, cholesteryi isopropyl ether, cholesteryi butyl ether, cholesteryl methoxymethyl ether, cholesteryl (2'-hydroxy)-3-ethyl ether) and cholesteryl ester (namely cholesteryi acetate) is tested on the interaction with phosphatidylcholines in liquid-crystalline and crystalline state. The interfacial properties of sterols are tested at the air-water interface. The cholesteryl ethers show a reduced interracial stability with increasing hydrophobicity of the ether-linked moiety. The interaction between the sterol derivatives and phospholipids in mixed monolayers is indicated by measuring the deviation from the simple addivity rule (condensing effect). An interaction is found only for cholesteryl (2'-hydroxy)-3-ethyl ether, cholesteryl methyl ether and cholesteryl ethyl ether. These sterols also reduce the glucose permeability of liposomal membranes in this order. In this respect cholesteryl (2'-hydroxy)-3-ethyl ether is as effective as cholesterol. Cholesteryl methyl ether and cholesteryl ethyl ether show 62 and 33 percent of the effect observed with cholesterol. The effect of the sterol derivatives on the gel-to-liquid-crystalline phase transition of dipalmitoylphosphatidylcholine is measured by differential scanning calorimetry. Cholesteryl methyl ether, cholesteryl ethyl ether, and cholesteryl (2'-hydroxy)-3-ethyl ether reduce the energy content of the phase transition nearly as effective as cholesterol, cholesteryl n-propyl ether has only a small effect. Although cholesteryl acetate, and cholesteryl methoxymethyl ether have no condensing or permeability-reducing effect, they have a considerable effect on the gel-to-liquid-crystalline phase transition. Cholesteryi isopropyl ether and cholesteryl butyl ether have no effect. It is concluded that a free 30-hydroxy group is not a prerequisite to observe a sterol-like effect in membranes. However, the interfacial stability and the orientation of the sterol and oxygen moiety at the sterol 3-position are important.

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Monolayer interactions of individual lecithins with natural sterols

Cited by 83 publications

References 10 publications

Comparison of membrane organization in mitochondria from yeast and rat liver by nuclear magnetic resonance spectroscopy

Comparison of membrane organization in mitochondria from yeast and rat liver by nuclear magnetic resonance spectroscopy

Order from disorder, corralling cholesterol with chaotic lipids

The effect of the sterol oxygen function on the interaction with phospholipids

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