Monolayer-Protected Nanoparticle Doped Xerogels as Functional Components of Amperometric Glucose Biosensors

Freeman, M.H.; Hall, Jori N.; Leopold, Michael C.

doi:10.1021/ac3037188

Cited by 54 publications

(99 citation statements)

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“…The sol-gels were aged at 50% RH for 48 h to form xerogels. Note: The environment during aging is of crucial importance, since sensor performance has been shown to vary drastically depending on time and RH [5,35]. SEM and electrochemical characterization of the xerogel layers has been either previously presented [5,35] or is included in Supplementary material herein.…”

Section: Fabrication and Characterization Of The Xerogel Biosensormentioning

confidence: 99%

“…Note: The environment during aging is of crucial importance, since sensor performance has been shown to vary drastically depending on time and RH [5,35]. SEM and electrochemical characterization of the xerogel layers has been either previously presented [5,35] or is included in Supplementary material herein. Additionally, prior work in the lab evaluated the porosity and surface area of drop-cast xerogel films, including films from silane precursors used in this report, using electrochemistry, spectroscopy, and contact angle goniometry [5].…”

Section: Fabrication and Characterization Of The Xerogel Biosensormentioning

confidence: 99%

“…The outer polyurethane (PU) layer semipermeable membrane was applied as previously reported [5,34,35]. Briefly, PU blends were prepared from dissolving different ratios of HPU and TPU in ethanol and THF, which was stirred overnight.…”

Section: Fabrication and Characterization Of The Xerogel Biosensormentioning

confidence: 99%

“…As in previous work [5,34,35], assembled sensors were pre-treated by being soaked in PBS (65.55 mM; pH = 7) for 1 h to ensure saturation of the xerogel matrix. The sensors then were immersed in 25 mL of stirred PBS (65.55 mM; pH = 7) for amperometric measurements wherein the working electrode potential was held at +0.65 V (vs. Ag/ AgCl, satrd' KCl) for 20 min.…”

Section: Evaluation Of Sensor Performancementioning

confidence: 99%

“…For example, Schoenfisch et al have successfully used layering methodology of sol-gel materials for effective, biocompatible glucose sensors [34]. Others have used LbL approaches with sol-gel materials to introduce nanoparticle networks that enhance the signal from glucose sensors [35]. In the context of UA biosensing, Ferreira et al [36] and Wang et al [37] reported layering of UOx within polymer films at indium tin oxide and layering of gold nanoparticle-doped choline at glassy carbon electrodes, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Layer-by-layer design and optimization of xerogel-based amperometric first generation biosensors for uric acid

Conway

Lambertson

Schwarzmann

et al. 2016

Journal of Electroanalytical Chemistry

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a b s t r a c tA layer-by-layer (LbL) strategy of modifying an electrode with a specific combination of polymeric and xerogel materials is used to create an effective first generation biosensor for uric acid (UA) -a clinically relevant molecule implemented in pregnancy induced hypertension (PIH) diagnosis, a condition that can lead to a serious disorder called preeclampsia. In addition to offering a new, promising sensor for UA, this study represents significant progress for amperometric biosensor development since the strategy and materials employed were successfully and readily adapted from a glucose biosensor model scheme and used for the effective detection of UA, a fundamentally different molecule compared to glucose. Specifically, each of four, functional modifying layers (outer polyurethane (PU) selective membrane, the inner selective electropolymer, and the xerogel bi-layer) are systematically investigated and tailored for UA permeability and interferent discrimination. The role of PU hydrophobicity and its UA permeability are established while the enzyme-doped and outer diffusional xerogel layers are evaluated for uricase (UOx) species/loading and silane precursor dependence, respectively. LbL systematic evaluation reveals the specific combination of hydroxyl-methyl triethoxy silane (HMTES) xerogels, a polyluminol-aniline electropolymer, and 100% hydrophilic polyurethane yields impressive uric acid sensing performance: effective sensitivity (0.8 nA/μM), linear response across physiologically relevant UA concentrations (100-700 μM), fast response times (~10 s), low limits of detection (b 10 μM), and selectivity against most common interferents. Toward the specific application of PIH risk assessment, the optimized sensor exhibited 10 day stability as well as effective shelf-life exceeding 35 days. The presented system rivals or exceeds UA biosensor performance found in the literature and offers the possibility of miniaturization for in situ or in vivo remote diagnostic sensing. The successful adaptation suggests that the strategy and materials may be applicable to detecting/monitoring other medically significant molecules via sensor development.

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Section: Fabrication and Characterization Of The Xerogel Biosensormentioning

confidence: 99%

Section: Fabrication and Characterization Of The Xerogel Biosensormentioning

confidence: 99%

Section: Fabrication and Characterization Of The Xerogel Biosensormentioning

confidence: 99%

Section: Evaluation Of Sensor Performancementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Layer-by-layer design and optimization of xerogel-based amperometric first generation biosensors for uric acid

Conway

Lambertson

Schwarzmann

et al. 2016

Journal of Electroanalytical Chemistry

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Noble Metal Construction for Electrochemical Nonenzymatic Glucose Detection

Asif

Liu

et al. 2022

Adv Materials Technologies

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transistor, [6] electrochemical glucose sensors with superior selectivity, sensitivity, and portability have aroused great interest of researchers. [7] In the past few decades, most researches have focused on the traditional glucose sensors, which are based on the immobilization of glucose oxidase on various substrates. [8,9] Among them, the catalytic oxidation of glucose is carried out under a certain voltage, and an electrical signal is output. These enzyme-based sensors display good selectivity and high sensitivity in glucose detection, but the enzyme activity is susceptible to pH, temperature, and toxic chemicals. [10] Nonenzymatic glucose sensors mainly depend on the current response of glucose directly oxidized on the surface of nanomaterials modified electrode, which shows higher stability than the enzyme-based glucose biosensor. [11] Therefore, great efforts have been made to develop novel nonenzymatic glucose sensors to solve the instability problem associated with enzyme-based catalysts. The low potential for catalyzing the glucose oxidation is critical for the stability of enzyme-free glucose sensors. Noble metal-based catalysts have been widely investigated for glucose sensing due to their lower redox potential, ranging between 0.1 and 0.5 V versus Ag/AgCl or saturated calomel electrode (SCE) reference electrodes, and high selectivity without interference from maltose, ascorbic, uric acid, and other biomolecules. Benefiting from these advantages, the noble metal-based glucose sensors also possess good stability and reproducibility. [12,13] However, noble metal catalysts with smooth surface and simple structure lack enough active sites, which directly affect the adsorption of glucose molecules and the desorption of glucose oxidation products, and further lead to poor sensitivity and stability of the enzyme-free glucose sensor. [14] Electrocatalytic glucose oxidation is a type of heterogenous catalytic reaction that occurs on the surfaces and interfaces of noble-metal-based nanostructures and electrolytes. It is generally believed that the activity, selectivity, and stability of electrocatalysts are sensitive to the surface and interface, which are not only related to their morphology, structure, and composition, but also closely related to the adsorption strength of reactant molecules or intermediates. [15,16] In this sense, each step should be beneficial to adsorption and desorption, thereby promoting the whole catalytic reaction. Sufficient active sites are the key to catalytic performance, which largely depends on the surface and interface state. In order to improve the catalytic ability of NM catalysts Precise glucose monitoring is needed to meet the growing demand of blood glucose determination in diabetes diagnosis. In spite of good selectivity and high sensitivity of enzyme-based glucose sensors, the activity of enzyme is sensitive to pH and temperature, which makes them unstable in detecting glucose level. Alternatively, nonenzymatic electrocatalysts have attracted intensive attention because o...

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Layered Xerogel Films Incorporating Monolayer‐Protected Cluster Networks on Platinum‐Black‐Modified Electrodes for Enhanced Sensitivity in First‐Generation Uric Acid Biosensing

2016

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Monolayer-Protected Nanoparticle Doped Xerogels as Functional Components of Amperometric Glucose Biosensors

Cited by 54 publications

References 50 publications

Layer-by-layer design and optimization of xerogel-based amperometric first generation biosensors for uric acid

Layer-by-layer design and optimization of xerogel-based amperometric first generation biosensors for uric acid

Noble Metal Construction for Electrochemical Nonenzymatic Glucose Detection

Layered Xerogel Films Incorporating Monolayer‐Protected Cluster Networks on Platinum‐Black‐Modified Electrodes for Enhanced Sensitivity in First‐Generation Uric Acid Biosensing

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