Monolithic media for applications in affinity chromatography

Abstract

Affinity chromatography presents a highly versatile analytical tool, which relies on exploiting highly specific interactions between molecules and their ligands. This review covers the most recent literature on the application of monoliths as stationary phases for various affinity-based chromatographic applications. Different affinity approaches as well as separations using molecularly imprinted monoliths are discussed. Hybrid stationary phases created by embedding of particles or nanoparticles into a monolithic stationary phase are also considered in this review article. The ease of preparation of monoliths and the multitude of functionalization techniques, which have matured during the past years, make monoliths interesting for an increasing number of biochemical and medical applications.

Recent advances in preparation and application of hybrid organic‐silica monolithic capillary columns

“…Their much higher external porosity compared to conventional particle-packed columns minimize the pore diffusion and mass transfer resistance, resulting in higher permeability and low-pressure drop with higher separation efficiency [1][2][3][4]. As a result, monolithic columns become an excellent tool in the analytical laboratory, not only for separation fields covering RP, ion-exchange, hydrophilic interaction, size exclusion, and affinity chromatography etc., but also for sample preparation including SPE or solid-phase microextraction (SPME), and as basis for immobilized enzyme reactors (IMERs) [5][6][7][8][9][10][11].…”

Recent advances and future trends in new materials for sample preparation

“…The MIPs have several advantages, such as high selectivity, specificity for the molecule used in the imprinting process, low cost of synthesis, thermal stability, higher physical strength, reusability, and robustness, and they allow pH variation [147]. MIPs have demonstrated promising applications in several areas {e.g., chemical/biological sensors [142], chromatographic stationary phases [143], chiral separation [152], antibody mimics [146], catalysts [147], and drugdelivery systems [148]}. MIPs have many applications in sample-preparation techniques as exemplified in several studies [135,158].…”

Macroporous polymers tailored as supports for large biomolecules: Ionic liquids as porogenic solvents and as surface modifiers

“…3 Organic polymer monoliths consisting of acrylamide-, methacrylate ester-, and styrenebased polymers have been successfully prepared. 4 However, creating a well-defined, homogeneous structure is a challenge in the preparation of monolithic materials. It is difficult to control the porous morphology of polymer monoliths prepared by traditional free radical polymerization because of the fast phase separation between the growing polymer chains and the porogenic solvents.…”