Monolithically integrated bacteriorhodopsin–GaAs/GaAlAs phototransceiver

Shin, Jonghyun; Bhattacharya, P.; Xu, Jian; Váró, György

doi:10.1364/ol.29.002264

Cited by 9 publications

(5 citation statements)

References 9 publications

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“…The almost molecular recognition used for fabrication of multilayers in this work, seems to be more efficient than the approach used for the generation of enhanced photovoltage produced by the stacking of hundreds of layers of loosely-oriented bacteriorhodopsin membrane patches. [19,20] Each of the bacteriorhodopsin proteins contains only a single chromophore, and a monolayer generates ca. 40 mV when excited by photons.…”

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confidence: 99%

Fabrication of Oriented Multilayers of Photosystem I Proteins on Solid Surfaces by Auto‐Metallization

et al. 2007

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Fabrication of serially-oriented multilayers of photosynthetic reaction center photosystem I (PS I) was mediated by the photo-catalytic specificity that reduced Pt 4+ ions to metal patches on the reducing side of PSI forming junctions with the oxidizing end of the proteins through Pt-sulfide bond of genetically-engineered cysteine mutants. The dry multilayers can be utilized in hybrid bio-solid-state electronic devices in which an increase in photo-voltage, resulting from the larger absorption cross-section and the serial-arrangement of PS I, is required. PS I is a transmembrane multisubunit protein-chlorophyll complex that mediates vectorial light-induced electron transfer. The nano-size dimension, an absorbed light energy yield of approximately 47% (or ca. 23% of solar radiation) and a photovoltage of 1 V with quantum efficiency of almost 1 [1] , make the reaction center a promising unit for applications in molecular nano-electronics. The robust PS I used in these experiments, that was isolated from the thylakoid membranes of cyanobacteria, is sufficiently stable to be used in hybrid solid-state electronic device. The dry PS I monolayer was shown earlier [3] to remain stable for more than three months and it stayed active for over one year in the present experiments. The structural stability is due to hydrophobic interactions that integrates 96 chlorophyll and 22 carotenoid pigment molecules and the trans membrane helixes of the core subunits.[2]The light-induced electron transfer at cryogenic temperatures [3] is an indication of little structural motions during function. We have fabricated self-assembled oriented monolayers by the formation of direct sulfide bonds between unique cysteine mutants of PS I from the cyanobacteria and the metal surface which generated, a photovoltage of 0.45 V under a dry environment. [4] In earlier works, only indirect adsorption of single plant PS I molecules [5] and binding of bacterial reaction center monolayers [6] were functioning in such an environment. Although a Schottky junction with PS I monolayer provides electronic coupling with unique photovoltaic properties, oriented multilayers can be advantageous when a larger light absorption cross section and enhanced photovoltage values are desired. As an efficient oriented multilayer, the PS I complexes need to be physically and electronically coupled and organized in a serial fashion. The use of the unique specificity of a photo-catalytic protein with redox potential of -0.53 V enabled the reduction of Pt 4+ ions and deposition of metallic platinum at the reducing end of PS I (Fig. 1a and b)

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Fabrication of Oriented Multilayers of Photosystem I Proteins on Solid Surfaces by Auto‐Metallization

et al. 2007

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“…PM shows functional response in both dry and humid conditions ͑following photocycles͒ and has been proposed as an excellent candidate for hybrid bioelectronic devices. [16][17][18] Patches of PM containing wild type bR were deposited onto a flat gold film evaporated on a mica substrate and dried under N 2 ͑g͒ flow. To avoid large current values, a resistor of 120 M⍀ was added in series with the sample.…”

Section: Nondestructive Thickness Measurement Of Biological Layers Atmentioning

confidence: 99%

Nondestructive thickness measurement of biological layers at the nanoscale by simultaneous topography and capacitance imaging

Casuso

Fumagalli

Gomila

et al. 2007

Applied Physics Letters

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Nanoscale capacitance images of purple membrane layers are obtained simultaneously to topography in a nondestructive manner by operating alternating current sensing atomic force microscopy in jumping mode. Capacitance images show excellent agreement with theoretical modeling and prove to be a noninvasive method for measuring the thickness of purple membrane layers beyond the single monolayer limit with nanoscale lateral spatial resolution. With the ability of spatially resolving the capacitance while preserving the sample from damaging, this technique can be applied for nanoscale thickness measurement of other biological layers and soft materials in general.

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“…bR has evolved as a protein extremely resistant to thermal, chemical, and photochemical degradations, which makes it one of the most promising candidates for the development of protein-based devices [1,5]. As such, it has been used for the development of photoreceivers [8], transceivers [9], optical switches [10], molecular engines [11], and electronic switches [12] and was also used for light-driven production of ATP [13]. bR is adapted to its biological function, but it should be re-engineered to meet the requirements for industrial hybrid materials used in competitive nanophotonic and photovoltaic applications.…”

Section: Introductionmentioning

confidence: 99%

Nano-biophotonic hybrid materials with controlled FRET efficiency engineered from quantum dots and bacteriorhodopsin

Bouchonville¹,

Cigne²,

Sukhanova³

et al. 2013

Laser Phys. Lett.

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Förster resonance energy transfer (FRET) between CdSe/ZnS core/shell quantum dots (QDs) and the photochromic protein bacteriorhodopsin (bR) in its natural purple membrane (PM) has been modulated by independent tuning of the Förster radius, the overlap integral between the donor emission spectrum and the acceptor absorption spectrum, and the distance between the donor (QD) and acceptor (bR retinal). The results have shown that the observed energy transfer from QDs to bR corresponds to that predicted by a multiple-acceptor geometric model describing the FRET phenomenon for QDs quasi-epitaxied on a crystalline lattice of bR trimers. Linking of QDs and bR via streptavidin–biotin linkers of different lengths improved FRET, with an efficiency as high as 82%, substantially exceeding the values predicted by the classical FRET theory. The data not only demonstrate the possibility of nano-bioengineering of efficient hybrid materials with controlled energy transfer properties, but also emphasize the necessity to develop an advanced theory of nano–bio energy transfer that would explain experimental effects contradicting the existing theoretical models.

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Monolithically integrated bacteriorhodopsin–GaAs/GaAlAs phototransceiver

Cited by 9 publications

References 9 publications

Fabrication of Oriented Multilayers of Photosystem I Proteins on Solid Surfaces by Auto‐Metallization

Fabrication of Oriented Multilayers of Photosystem I Proteins on Solid Surfaces by Auto‐Metallization

Nondestructive thickness measurement of biological layers at the nanoscale by simultaneous topography and capacitance imaging

Nano-biophotonic hybrid materials with controlled FRET efficiency engineered from quantum dots and bacteriorhodopsin

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