Monomer‐scale design of functional protein polymers using consensus repeat sequences

Chang, Marina P.; Huang, Winnie; Danielle, J.

doi:10.1002/pol.20210506

Cited by 12 publications

(8 citation statements)

References 265 publications

(335 reference statements)

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“…Indeed, the super-elastic properties of resilin are well known to play essential mechanical roles in arthropods, assisting movement and protecting compartments engaged in movement [ 36 , 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

The Bright Side of the Tiger: Autofluorescence Patterns in Aedes albopictus (Diptera, Culicidae) Male and Female Mosquitoes

Croce

Scolari

2022

Molecules

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Light-based events in insects deserve increasing attention for various reasons. Besides their roles in inter- and intra-specific visual communication, with biological, ecological and taxonomical implications, optical properties are also promising tools for the monitoring of insect pests and disease vectors. Among these is the Asian tiger mosquito, Aedes albopictus, a global arbovirus vector. Here we have focused on the autofluorescence characterization of Ae. albopictus adults using a combined imaging and spectrofluorometric approach. Imaging has evidenced that autofluorescence rises from specific body compartments, such as the head appendages, and the abdominal and leg scales. Spectrofluorometry has demonstrated that emission consists of a main band in the 410–600 nm region. The changes in the maximum peak position, between 430 nm and 500 nm, and in the spectral width, dependent on the target structure, indicate the presence, at variable degrees, of different fluorophores, likely resilin, chitin and melanins. The aim of this work has been to provide initial evidence on the so far largely unexplored autofluorescence of Ae. albopictus, to furnish new perspectives for the set-up of species- and sex-specific investigation of biological functions as well as of strategies for in-flight direct detection and surveillance of mosquito vectors.

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Section: Discussionmentioning

confidence: 99%

The Bright Side of the Tiger: Autofluorescence Patterns in Aedes albopictus (Diptera, Culicidae) Male and Female Mosquitoes

Croce

Scolari

2022

Molecules

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“…Multi-site ncAA incorporation is particularly desirable for the design and production of protein-based polymers (PBPs), which consist of tandem repeats of either natural or artificial short peptide motifs, and whose properties are determined by the sequence of the AAs and ncAAs in these motifs ( Connor and Tirrell, 2007 ; Israeli et al, 2019 ; Varanko et al, 2020 ; Chang et al, 2021 ). Although the incorporation of ncAAs in numerous PBPs can be used to expand their range of properties and functions ( Connor and Tirrell, 2007 ; Israeli et al, 2019 ), we focus here on the effect of ncAA incorporation on the properties of two families of bio-inspired, thermo-responsive PBPs: elastin-like polypeptides (ELPs) and resilin-like polypeptides (RLPs).…”

Section: Introductionmentioning

confidence: 99%

Tuning the Properties of Protein-Based Polymers Using High-Performance Orthogonal Translation Systems for the Incorporation of Aromatic Non-Canonical Amino Acids

Gueta

Sheinenzon

Azulay

et al. 2022

Front. Bioeng. Biotechnol.

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The incorporation of non-canonical amino acids (ncAAs) using engineered aminoacyl-tRNA synthetases (aaRSs) has emerged as a powerful methodology to expand the chemical repertoire of proteins. However, the low efficiencies of typical aaRS variants limit the incorporation of ncAAs to only one or a few sites within a protein chain, hindering the design of protein-based polymers (PBPs) in which multi-site ncAA incorporation can be used to impart new properties and functions. Here, we determined the substrate specificities of 11 recently developed high-performance aaRS variants and identified those that enable an efficient multi-site incorporation of 15 different aromatic ncAAs. We used these aaRS variants to produce libraries of two temperature-responsive PBPs—elastin- and resilin-like polypeptides (ELPs and RLPs, respectively)—that bear multiple instances of each ncAA. We show that incorporating such aromatic ncAAs into the protein structure of ELPs and RLPs can affect their temperature responsiveness, secondary structure, and self-assembly propensity, yielding new and diverse families of ELPs and RLPs, each from a single DNA template. Finally, using a molecular model, we demonstrate that the temperature-responsive behavior of RLPs is strongly affected by both the hydrophobicity and the size of the unnatural aromatic side-chain. The ability to efficiently incorporate multiple instances of diverse ncAAs alongside the 20 natural amino acids can help to elucidate the effect of ncAA incorporation on these and many other PBPs, with the aim of designing additional precise and chemically diverse polymers with new or improved properties.

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“…Since the establishment of the concept of macromolecule, 1,2 synthesis of new polymers and the study on their structureproperty relationship have always been the focus of polymer science. [3][4][5][6][7][8][9] Controlled synthesis of tailor-made polymers is of great significance in both academia and industry. 10,11 Polyethylene (PE) is arguably the most simple and useful polymer that produced in large amounts.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of precision poly(1,3‐bicyclo[1.1.1]pentane alkylene)s via acyclic diene metathesis polymerization

Cheng

2023

Journal of Polymer Science

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1,3-Disubstituted bicyclo[1.1.1]pentane (BCP) is a rigid, linear, non-conjugated hydrocarbon unit that is usually transformed from [1.1.1]propellane. This unit has been introduced into drugs as mimics of 1,4-disubstituted benzene moieties and used as rigid linkers in small-molecule materials. However, the influence of this unique structure on the polymer properties has only been scarcely investigated. In this work, three symmetrical α,ω-diene monomers containing one and two BCP units were synthesized for the first time from [1.1.1]propellane. Three types of precise poly(1,3-bicyclo[1.1.1]pentane alkylene)s with BCPs located on every 11th and 21st chain carbons were obtained via acyclic diene metathesis (ADMET) polymerization and subsequent hydrogenation. All these polymers show higher thermal stability than linear polyethylene. For polymers containing one BCP moiety in their repeating unit, BCP acts as a defect to distort the PE crystals and decrease the melting temperature of PE. However, the polymer that containing a [2]staffane moiety (two jointed BCP units) in the polymer backbone forms a new crystalline morphology. This work elucidates the impact of BCP units on the thermal and crystallization behavior of PE, providing new insights of utilizing BCP unit to construct new polymer backbone, regulate the packing order of polymers and their properties.

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Monomer‐scale design of functional protein polymers using consensus repeat sequences

Cited by 12 publications

References 265 publications

The Bright Side of the Tiger: Autofluorescence Patterns in Aedes albopictus (Diptera, Culicidae) Male and Female Mosquitoes

The Bright Side of the Tiger: Autofluorescence Patterns in Aedes albopictus (Diptera, Culicidae) Male and Female Mosquitoes

Tuning the Properties of Protein-Based Polymers Using High-Performance Orthogonal Translation Systems for the Incorporation of Aromatic Non-Canonical Amino Acids

Synthesis of precision poly(1,3‐bicyclo[1.1.1]pentane alkylene)s via acyclic diene metathesis polymerization

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