Monomeric (7S) IgM found in the serum of rheumatoid arthritis patients share idiotypes with pentameric (19S) monoclonal rheumatoid factors.

Bonagura, Vincent R.; Mendez, LM Gomez; Agostino, Nicodemo; Pernis, Benvenuto

doi:10.1172/jci112888

Cited by 12 publications

(7 citation statements)

References 23 publications

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“…'2 13 These RCRI+7S IgM molecules inhibited binding of affinity purified anti-RCRI polyclonal antibodies with some RCRI+ pokeweed mitogen plasma cells obtained from RF+ patients with RA expressing the RCRI as a dominant idiotype in pokeweed mitogen culture.12 Two populations of RCRI+ pokeweed mitogen plasma cells were therefore identified: one closely related to the monoclonal IgM RF dominant idiotype defined by Waldenstrom's macroglobulinaemia prototypes, and another background population, found in similar prevalence both in patients with RA and in control individuals. 12 We now describe a similar observation in RF-patients with RA.…”

Section: Rheiumatoid Factor Cross Reactive Idiotype Expression 489supporting

confidence: 72%

“…' 12 In addition, low molecular weight 7S IgM obtained from some RF+ adults with RA is marked by the major RCRI yet fails to bind human or rabbit IgG in classic 'functional' binding assays. 12 15 In the present communication we have identified a group of RFadults with RA who generate pokeweed mitogen plasma cells which express the major RCRI as a dominant idiotype. We have previously described a similar finding in RF+ patients with RA.…”

Section: Discussionmentioning

confidence: 81%

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Seronegative rheumatoid arthritis, rheumatoid factor cross reactive idiotype expression, and hidden rheumatoid factors.

Bonagura

Wedgwood²,

Agostino³

et al. 1989

Annals of the Rheumatic Diseases

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Section: Rheiumatoid Factor Cross Reactive Idiotype Expression 489supporting

confidence: 72%

Section: Discussionmentioning

confidence: 81%

Seronegative rheumatoid arthritis, rheumatoid factor cross reactive idiotype expression, and hidden rheumatoid factors.

Bonagura

Wedgwood²,

Agostino³

et al. 1989

Annals of the Rheumatic Diseases

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“…However, while the Wa and Po CRI have been detected in polyclonal rheumatoid factors present in the sera of patients with rheumatoid arthritis (21)(22)(23)(24), the amounts of these CRI are quite modest. Extensive serologic analyses of polyclonal rheumatoid factors from patients with rheumatoid arthritis show them to be diverse, and that the common idiotypes ofthe mixed cryoglobulin rheumatoid factors account for only a minor population (25,26).…”

Section: Introductionmentioning

confidence: 99%

Rheumatoid factors isolated from patients with autoimmune disorders are derived from germline genes distinct from those encoding the Wa, Po, and Bla cross-reactive idiotypes.

Victor¹,

Randen²,

Thompson³

et al. 1991

J. Clin. Invest.

104

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To better understand the structural basis for rheumatoid factor activity, the nucleotide sequence of the light chain variable regions of nine human monospecific IgM rheumatoid factors were analyzed. Rheumatoid factors were isolated from three patients with rheumatoid arthritis, a patient with systemic lupus erythematosus, and a normal individual. The VL gene segments used by these rheumatoid factors are not as restricted as previous work on mixed cryoglobulin rheumatoid factors had suggested. Each of the different VK families is represented and there are two examples where a V, gene segment is used. Molecules with structures similar to those of the Wa and Po CRI, characteristic of mixed cryoglobulin rheumatoid factors, are not common among these rheumatoid factors isolated from patients with rheumatoid arthritis. While there are clear examples of rheumatoid factors that are direct copies of germline genes, most of the sequence data suggest that the processes of antigenic selection and somatic mutation contribute significantly to the generation of monospecific rheumatoid factors in patients with autoimmune disease. (J. Clin. Invest. 1991.

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“…Of 10 RF-F&4 individuals, five expressed high frequencies of RCRI+ PWM-PCs comparable to those observed in RF+ RA patients, TAble I (12,13). Of 10 RF-F&4 individuals, five expressed high frequencies of RCRI+ PWM-PCs comparable to those observed in RF+ RA patients, TAble I (12,13).…”

Section: Detection Of Classic Rf Igg Binding In Ra Pnrient Ctnd Contrmentioning

confidence: 81%

“…All had RA by ARA criteria (12,13) had active sustained disease for a minimum of two years, and had received, or were currently receiving, second-line drug therapy. All had RA by ARA criteria (12,13) had active sustained disease for a minimum of two years, and had received, or were currently receiving, second-line drug therapy.…”

Section: Hwnnn Subjectsmentioning

confidence: 99%

Cellular Expression of Rheumatoid Factor Cross-reactive Idiotype in Patients with Seropositive and Seronegative Rheumatoid Arthritis

Pernis

Bonagura

1988

Scandinavian Journal of Rheumatology

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A study has been made of the presence of the major rheumatoid Factor cross-reacting idiotype (RCRI) in the serum of patients with seropositive and seronegative rheumatoid arthritis a s well a s in the cytoplasm of plasmacells induced by cultivation of peripheral blood leucocytes (PBL) from the same RA patients and from normal humans. The PBL were stimulated in wire with two different 13 cell niitogens, namely pokeweed mitogen (PWM) or Staph Auerus Cowan strain I (SAC).The RCRI was detected in the serum of seropositive RA patients and in the scruni of four out of twenty seronegative RA patients. In these four, however, the seronegative condition was only apparent, since all four had hidden rheumatoid factors that could be shown after dissociation from IgG. The expression of the RCRI in the cytoplasm of PWM-stimulated PBL from seronegative RA patients was concordant with the expression in the serum. Thus the majority of truly seronegative RA patients also appear not to express the RCRI neither in the serum nor in the cytoplasm of plasmacells induced by PWM stimulation of PBL.An unexpected finding was the detection of the RCRI in plasmacells obtained by SAC stimulation of PBL from normal individuals. U p to 40% of RCRI-positive plasmacells were seen in many of these cultures, as opposed to a n average of 2% in cultures stimulated with PWM from PBL of the same individuals. High percentages of RCRI-positive plasma-cells have previously been detected by us in PWM cultures only if these were from PBL from RA patients.Our working hypothesis is that PWM is a non-selective stin~ulator of B cells and that R C R I f precursors are in high frequency in RA patients, whereas SAC selectively stimulates these precursors even in normal PBL populations, where their frequency is not abnormally high. Scand J Rheumatol Downloaded from informahealthcare.com by Chinese University of Hong Kong on 02/08/15 For personal use only. Scand J Rheumatol Downloaded from informahealthcare.com by Chinese University of Hong Kong on 02/08/15 For personal use only.Appearance of RCM+ P W -P C s , induced j?om RF-, RA yntienrs' PBM RF-RApatients or control PBM cultivated for 6 d with PWM induced B lymphocyte maturation to PCs. These PWM-PCs contained intracytoplasmic immunoglobulin demonstrable Scand J Rheumatol Downloaded from informahealthcare.com by Chinese University of Hong Kong on 02/08/15 For personal use only. Scand J Rheumatol Downloaded from informahealthcare.com by Chinese University of Hong Kong on 02/08/15 For personal use only. Scand J Rheumatol Downloaded from informahealthcare.com by Chinese University of Hong Kong on 02/08/15 For personal use only. Scand J Rheumatol Downloaded from informahealthcare.com by Chinese University of Hong Kong on 02/08/15 For personal use only. 134:3281-5. Scand J Rheumatol Downloaded from informahealthcare.com by Chinese University of Hong Kong on 02/08/15 For personal use only.

show abstract

Monomeric (7S) IgM found in the serum of rheumatoid arthritis patients share idiotypes with pentameric (19S) monoclonal rheumatoid factors.

Abstract: Serum from some seropositive (RF +) rheumatoid arthritis (RA)

Cited by 12 publications

References 23 publications

Seronegative rheumatoid arthritis, rheumatoid factor cross reactive idiotype expression, and hidden rheumatoid factors.

Seronegative rheumatoid arthritis, rheumatoid factor cross reactive idiotype expression, and hidden rheumatoid factors.

Rheumatoid factors isolated from patients with autoimmune disorders are derived from germline genes distinct from those encoding the Wa, Po, and Bla cross-reactive idiotypes.

Cellular Expression of Rheumatoid Factor Cross-reactive Idiotype in Patients with Seropositive and Seronegative Rheumatoid Arthritis

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