Monomethylated arsenic was the Major methylated arsenic in Red blood cells of acute promyelocytic leukemia patients treated with arsenic trioxide

Arsenic-Related Health Risk Assessment of Realgar-Containing NiuHuangJieDu Tablets in Healthy Volunteers Po Administration

“…In our study, no traces of arsenic speciation were found in the human plasma because the absorption of arsenic was considerably low after oral administration of NHJDT, and the limited amount of arsenic in the blood was substantially bound to red blood cells (Lu et al, 2007;Nicolis et al, 2009;Yoshino et al, 2009;Chen et al, 2013). High affinity of arsenic with human hemoglobin was responsible for the accumulation of arsenic in the blood of APL patients (Guo et al, 2021), which was consistent with our study. Therefore, the whole blood total arsenic level is confirmed to be a more reliable indicator than that of plasma for arsenic-related health risk assessment.…”

“…No arsenic species was found above the LODs (1 ng/ml) in the plasma for all the components under investigation after both the single and multiple doses oral administration (Figure 3). As reported in a previous study, DMA, MMA, and inorganic arsenics were detected in the plasma of acute promyelocytic leukemia (APL) patients after daily intravenous administration of arsenic trioxide at 0.16 mg/kg (Guo et al, 2021). In our study, no traces of arsenic speciation were found in the human plasma because the absorption of arsenic was considerably low after oral administration of NHJDT, and the limited amount of arsenic in the blood was substantially bound to red blood cells (Lu et al, 2007;Nicolis et al, 2009;Yoshino et al, 2009;Chen et al, 2013).…”

“…As shown in Figures 4D,E, DMA can still be detected in human urine 10 days after the single dose or 38 days after the multiple doses. The slow elimination of arsenics from the human body is clinically once more proven because of the strong protein and tissue binding (Guo et al, 2021). Thence, precautions should be taken to avoid excessive or long-term medication of realgar-containing TCMs to prevent high levels of arsenic exposure.…”

Atomic spectrometry update: review of advances in the analysis of clinical and biological materials, foods and beverages

“…In the ongoing studies by Hai and co-workers on aspects of arsenic trioxide treatment of patients suffering from acute promyelocytic leukemia, relevant As species were determined by HPLC-HG-AFS. In the latest in a series of papers, the analyses of CSF, 107 plasma 108 and red blood cells 109 were described. Although each paper contains all the details of sample preparation necessary to replicate the work, there is little information about the HPLC-HG-AFS part of the analysis.…”

Atomic Spectrometry Update: review of advances in elemental speciation

“…A method to determine As species in red blood cells collected from 97 APL patients has also been reported. 78 A mixture of H 2 O 2 and HClO 4 was used to release the haemoglobin bound As and precipitate protein. Four As species, As III , As V , MMA V and DMA V , in plasma and red blood cells were then detected by HPLC-HG-AFS.…”