Monomolecular enzyme films stabilized by amphiphilic polyelectrolytes for biosensor devices

Eremenko, A. V.; Kurochkin, Ilya N.; Chernov, S. F.; Barmin, A. V.; Yaroslavov, Alexander A.; Moskvitina, T. A.

doi:10.1016/0040-6090(94)06403-2

Cited by 18 publications

(6 citation statements)

References 8 publications

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“…If a layer of an oppositely charged amphiphile is then spread on the water surface and formed into an LB film, the biomolecule will then be incorporated into the LB film to obtain the nanostructured biointerface. Various enzymes, such as HRP, 150 glucose oxidase, 151 and urease, 152 solid substrate followed by crosslinking with glutaraldehyde. 153 When amine LB films were deposited from a sub-phase containing dissolved DNA, the DNA was unsurprisingly incorporated into the LB film.…”

Section: Assembly and Characterizations Of Nanostructured Biointerfacesmentioning

confidence: 99%

Interfacial Bioelectrochemistry: Fabrication, Properties and Applications of Functional Nanostructured Biointerfaces

2006

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Bioelectrochemistry is a new interdisciplinary field, which combines biotechnology with electrochemical science, while the emergence of nanotechnology is opening new horizons for the investigation of this field. Nanostructures (such as nanoparticles, nanotubes) have similar dimensions to those of biomolecules (such as proteins or DNA). The combination of nanostructures with biomolecules yields functional nanostructured biointerfaces with synergetic properties and functions. The recent surge of research interest in the bioelectrochemical field is focused on the advanced design and preparation of such potential nanostructured biointerfaces, as well as the fundamental understanding of the interfacial bioelectrochemical processes within these systems. This review describes recent advances in the development of nanostructured biointerfaces based on the nanostructure-biomolecule hybrid systems in the bioelectrochemical field. To begin with, a wide range of nanostructures, which derive from metal, semiconductor, carbon, and so on, are discussed, involving their synthesis, properties, and conjugation with biomolecules, respectively. Sequentially, the fabrication, properties and characterization of functional nanostructured biointerfaces on electrode surfaces are discussed. Particular emphasis is directed to the discussion and exploration of interfacial bioelectrochemical theories of these architectures, which are of fundamental importance to the unraveling of key biological processes and the comprehensive acquaintance of electron-transfer processes. In addition, the wide applications of nanostructured biointerfaces in the bioelectrochemical field are sketched, and prospects and further developments in this exciting interdisciplinary field are also suggested.

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Section: Assembly and Characterizations Of Nanostructured Biointerfacesmentioning

confidence: 99%

Interfacial Bioelectrochemistry: Fabrication, Properties and Applications of Functional Nanostructured Biointerfaces

2006

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“…The layer-by-layer (LBL) − and Langmuir−Blodgett (LB) film deposition techniques are two major immobilization methods for OPH. They have potential industrial applications in the development of biosensors because well-ordered, stable films with high strength are formed. − The unique native fold of an enzyme is crucial to its activity. Therefore, upon immobilization, the enzyme conformation must be maintained for optimal performance of the biosensor.…”

Section: Introductionmentioning

confidence: 99%

Secondary Structure of Organophosphorus Hydrolase in Solution and in Langmuir−Blodgett Film Studied by Circular Dichroism Spectroscopy

et al. 2004

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The secondary structure of organophosphorus hydrolase (OPH) has been studied with circular dichroism (CD) spectroscopy in the far-UV region. The effect of pH on the secondary structure of OPH solution was examined over the pH range from 3.56 to 9.60. As shown on the CD spectra, the secondary structure of OPH is well defined when the pH value is near the isoelectric point (7.6); however, it is destroyed when the pH values are increased or decreased further. This is explained by the loss of helical structure. The pH effect on CD spectra contributes to clarify the optimum pH needed to obtain a stable OPH Langmuir film at the airwater interface and its correlation to the secondary structure of the enzyme. Comparative study of the thermal treatment on the secondary structure of OPH in solution, Langmuir-Blodgett film, and dry film shows that the molecular arrangement plays a dominant role in the thermal stability of OPH. With use of the CDPro software package a quantitative estimation of the secondary structure from the CD spectra of OPH solution was obtained. Results show that there is a decrease in the percentage of the R-helical and an increase of β-strands with the change of pH or temperature.

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“…81 For the immobilization of biomolecules in polyelectrolyte complexes, it has been reported that immobilized enzymes retain higher activities than native enzymes, 82,83 and were stable. 82,84 From these reports, it is also evident that polyelectrolyte complexes are suitable materials for immobilizing biomolecules. The main focus of the preceding discussion was the application of polyelectrolyte membranes to biosensors and enzyme electrodes.…”

Section: ·5 Other Properties and Applicationsmentioning

confidence: 99%

Polyelectrolyte Complex Membranes for Immobilizing Biomolecules, and Their Applications to Bio-analysis

Yabuki

2011

ANAL. SCI.

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The immobilization of biomolecules is an important technique for bio-analysis, and can be applied to biosensors with both high selectivity and high sensitivity. Many researchers have developed immobilization techniques to optimize these characteristics. In the last two decades, an immobilization technique that meets the desired requirements was developed by using polyelectrolytes to form complexes, based on the electrostatic binding between polycations and polyanions. This review summarizes the techniques used for the immobilization of biomolecules by polyelectrolyte complexes; it also discusses related subjects.

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Monomolecular enzyme films stabilized by amphiphilic polyelectrolytes for biosensor devices

Cited by 18 publications

References 8 publications

Interfacial Bioelectrochemistry: Fabrication, Properties and Applications of Functional Nanostructured Biointerfaces

Interfacial Bioelectrochemistry: Fabrication, Properties and Applications of Functional Nanostructured Biointerfaces

Secondary Structure of Organophosphorus Hydrolase in Solution and in Langmuir−Blodgett Film Studied by Circular Dichroism Spectroscopy

Polyelectrolyte Complex Membranes for Immobilizing Biomolecules, and Their Applications to Bio-analysis

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