Monophasic molybdenum selenide-reduced graphene oxide nanocomposite sheets based immunosensing platform for ultrasensitive serotonin detection

Multiwalled carbon nanotube nanofiller-polyindole polymer matrix-based efficient biosensor for the rapid detection of swine flu

“…3c]. This indicates the quasi-reversible electron transfer behavior 55 and follows eqn (5) and (6), suggesting that the rate of mass transfer is equal to the rate of electron transport.Δ E p(APTES/PIN/MTs/ITO) = [0.028 ± 0.0006 V (mV s −1 ) 1/2 × { ν (mV s −1 )} 1/2 ] + 0.138 ± 0.005 V R 2 = 0.99Δ E p (BSA/anti-SAA/APTES/PIN/MTs/ITO) = [0.027 ± 0.0006 V (mV s −1 ) 1/2 × { ν (mV s −1 )} 1/2 ] + 0.13 ± 0.005 V, R 2 = 0.99…”

“…This can be attributed to the presence of an electrostatic charge on the antibodies (-NH 2 ) fragments, which results in better electron diffusion. 55 Also, the good orientation and better immobilization of anti-SAA onto the APTES/PIN/MTs/ITO electrode contributes to rapid electron transfer between the solution and the electrode. 56…”

Nanostructured Mesoporous Carbon Based Electrochemical Biosensor for Efficient Detection of Swine Flu

“…For the validation of specific ligand activity of antigen and antibody towards each other, a control experiment has also been performed by using APTES/mPC/ITO electrode instead of BSA/anti-SAA/APTES/mPC/ITO biosensing electrode however other experimental conditions kept same as in electrochemical biosensing response studies. The obtained results are shown in Figure 4e, revealed that no substantial shift in the magnitude of the anodic peak current of APTES/mPC/ITO electrode with the increase in the concentration of SAA antigen from 30 μg mL À 1 to 70 μg mL À 1 , indicating that SAA shows no interaction with APTES/mPC/ITO electrode and it specifically binds with anti-SAA biomolecules immobilized onto the biosensing electrode [59]. Further, to investigate the cross-reactivity of biosensing electrode towards the other biomolecules such as glucose, urea, annexin, etc.…”

“…present in the serum sample of swine flu patients, interferents study has been conducted through CV technique and the obtained results are shown in Figure S6. Initially, we added 60 μL of glucose (7 mg mL À 1 ), uric acid (1 mg mL À 1 ), NaCl (1 mM), and annexin (20 μg mL À 1 ) molecules one by one in the experimental solution and providing at 12 min as incubation time [23]. Further, we recorded the current response through CV techniques, concluded that no significant changes (the average mean %RSD obtained by adding glucose, uric acid, NaCl and annexin is 0.92 %) have been observed in the peak current obtained through the BSA/ anti-SAA/APTES/mPC/ITO electrode.…”

“…[22] Carbonaceous nanomaterial predominantly graphene and its oxygenated derivative (GD) i. e., graphene oxide (GO) and reduced graphene oxide (rGO). The GO and rGO endowed with peculiar physical properties, such as high mechanical efficiency, specific surface area, flexibility, constancy in a physiological environment, and plenty of functional groups, have enormously employed and showed pioneer applications in nanoelectronics, composite materials, energy storage technology, electrode material, bioimaging, biosensing [22,23], drug delivery and cancer therapy [24]. Nevertheless, in consideration of physico-chemical properties of GD towards their differential in vitro behavior in human lung cells, thermally reduced graphene oxide (TRGO) shows an adverse effect on human lung (normal or cancer) cells, majorly on cellular viability, cell death, genotoxicity, and oxidative stress [24].…”