Monopronucleated (<scp>1PN</scp>) and tripronuclear (<scp>3PN</scp>) zygotes formation during assisted reproduction in <scp>POSEIDON</scp> group 4 patients: Emphasizing on polar bodies

Tsai, Ni-Chin; Su, Yu-Ting; Lin, Yu‐Jen; Chiang, Hsin‐Ju; Huang, Fu-Jen; Kung, Fu‐Tsai; Huang, Hung‐Yu; Lan, Kuo‐Chung

doi:10.1111/jog.14910

Cited by 5 publications

(2 citation statements)

References 43 publications

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“…Immuno uorescent analysis shows that most of 3PN zygotes derived from c-IVF had a female PN (fPN)and two male PNs (mPN), suggesting polyspermy [9]. On the other hand, 3PN zygotes after ICSI showed two fPNs and a mPN, indicating failure of the extrusion of the second polar body [4,9,10,11]. In the 3PN embryos after ICSI, equal ratios of XXX and XXY were observed and no XYY embryos were present [12].…”

Section: Discussionmentioning

confidence: 99%

Effect of three pro-nuclei (3PN) on the outcomes of PGT

Jiang

Yuan

et al. 2023

Preprint

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Aims To explore the influence of 3PN on outcomes in preimplantation genetic testing (PGT) cycles. Methods This was a retrospective study of patients undergoing PGT treatment included 139 PGT for aneuploidy (PGT-A) cycles and 285 cycles PGT for monogenic/single gene defects and/or chromosomal structural rearrangements (PGT-M/SR). The 424 PGT cycles were divided into two groups as follows: group 1 included 343 cycles with no 3PN zygotes and group 2 included 81 cycles with 3PN zygotes. 3PN cycle rate was compared between PGT-A and PGT-M/SR cycles. The outcomes of PGT were analyzed between the two groups. Results The female and male's age were significantly higher in PGT-A than PGT-M/SR cycles. Whereas 3PN rate was not significantly different between the two groups. The number of retrieved oocytes was significantly higher in 3PN group than Non 3PN group (13.98 ± 7.10 vs. 10.89 ± 6.65; P < 0.05). The MII oocyte rate, 2PN fertilizaion rate, D3 high embryo rate, biopsy blastocyst rate, per oocyte utilization and D5 blastocyst rate were significantly lower in 3PN group than Non 3PN group (P < 0.05). The results of chromosomal mosaic rate was significantly higher in 3PN group than Non 3PN group (16.3 vs. 11.9; P < 0.05). Conclusions No correlation was observed between 3PN formation rate and PGT-A or PGT-M/SR cycles. The occurrence of 3PN seems to impair the developing blastocyst and interfere with good embryo formation rate and mosaic rate in PGT. But the occurrence of 3PN does not seem to impair the euploid rate and aneuploid rate.

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Section: Discussionmentioning

confidence: 99%

Effect of three pro-nuclei (3PN) on the outcomes of PGT

Jiang

Yuan

et al. 2023

Preprint

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“…The dose of gonadotropin was adjusted based on the individual response. After the maturation of follicles, oocytes were retrieved, and standard IVF or ICSI procedures were used for fertilization, as previously described [25,26].…”

Section: Protocolsmentioning

confidence: 99%

The effectiveness of vitrified zygote accumulation for managing the extremely diminished ovarian reserve in infertile women: a retrospective cohort study

Huang,

Tsai,

Lin

et al. 2024

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Background Accumulated vitrified embryos are stored for later thawed transfer to deal with poor responders. However, the cost and outcome of vitrified zygotes have yet to be studied. Our study aimed to investigate the effectiveness of the vitrified zygote accumulation method in diminished ovarian reserve patients. Methods This retrospective study included 122 diminished ovarian reserve patients who met the Poseidon classification groups 3 and 4 from June 1, 2019, to December 31, 2022. The patients underwent controlled ovarian stimulation and embryo transfer using accumulated vitrified zygotes. The clinical pregnancy rate and live birth rate per embryo transfer and stimulation cycle are evaluated. Results Two hundred seventy-eight IVF/ICSI cycles in one hundred twenty-two females treated at our institution were divided into two groups, according to age < 40 years (n = 51) and ≥ 40 years (n = 71). The younger group had significantly more favorable outcomes than the older group only in live birth rates per cycle (12.6% vs. 4%, p = 0.029) and per transfer (24.1% vs. 9.9%, p = 0.027). However, the older group had more embryos per transfer (2.4 ± 0.9 vs. 2.0 ± 0.8, p = 0.014). There were no differences between the number of stimulation cycles per patient, the number of oocytes and mature oocytes retrieved, the number of transfer cycles per patient, the cost per patient, or transfer. We set a model mimicking the cost of fresh embryos. The zygote accumulation strategy costs were higher per patient and per cycle than those of the fresh embryo transfer model. Conclusions In diminished ovarian reserve women undergoing IVF/ICSI treatment, younger women had significantly better live birth rates than the older women applying the vitrified zygote accumulation strategy, even though the older group had a higher number of embryos per transfer. However, the zygote accumulation strategy benefits older women more than fresh embryo transfer. The strategy might cost more but decrease the anxiety and stress.

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