Monounsaturated fatty acid-enriched olive oil exacerbates chronic alcohol-induced hepatic steatosis and liver injury in C57BL/6J mice

Guo, Rui; Lin, Chen; Zhu, Jinyan; Li, Jiaomei; Ding, Qiong; Chang, Kaixin; Han, Qiang; Li, Songtao

doi:10.1039/d2fo03323b

Cited by 7 publications

(5 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[37] More research has revealed that dietary fat is intimately linked to the formation and progression of chronic metabolic disorders in recent years. [38][39][40][41] Different types of fatty acids can interplay the host health improvement or disease through modifying the composition of gut microbiota. [42] TKO contain approximately 42.6-61.5% oil, and unsaturated fatty acids account for 76.1-94.3% of the total fatty acids.…”

Section: Discussionmentioning

confidence: 99%

Torreya grandis Kernel Oil Alleviates Loperamide‐Induced Slow Transit Constipation via Up‐Regulating the Colonic Expressions of Occludin/Claudin‐1/ZO‐1 and 5‐HT3R/5‐HT4R in BALB/c Mice

Wang,

Guo,

et al. 2023

Molecular Nutrition Food Res

Self Cite

View full text Add to dashboard Cite

ScopeTorreya grandis kernel has traditionally been used to remove intestinal parasites and increases intestinal motility. However, the effect of Torreya grandis kernel oil (TKO) on constipation has not yet been investigated. Therefore, mouse model is used to investigate the effect of TKO on slow transit constipation (STC) and its possible mechanism.Methods and resultsThe effects of TKO on intestinal motility of STC mice are evaluated by fecal weight, fecal water content, colon length, defecation test, and intestinal propulsion test. The mechanism of TKO alleviating STC is explored by detecting biochemical analysis, histological analysis, western blot, qRT‐PCR, immunohistochemistry, and gut microbiota analysis. The results reveal that TKO effectively promotes defecation and intestinal motility, increases the level of endothelin‐1, and restores the histopathological morphology of the colon under LOP pretreatment. The expression levels of occludin, claudin‐1, and zonula occludens‐1 (ZO‐1) mRNA and protein are up‐regulated in mice receiving TKO treatment. The colonic 5‐hydroxytryptamine 3R/4R (5‐HT3R/5‐HT4R) expressions are also increased by TKO supplementation. Additionally, TKO rescues LOP‐caused disorders of the gut microbiota.ConclusionConsumption of TKO is beneficial to STC recovery, and it can alleviate LOP‐induced STC by up‐regulating the colonic expressions of Occludin/Claudin‐1/ZO‐1 and 5‐HT3R/5‐HT4R.

show abstract

Section: Discussionmentioning

confidence: 99%

Torreya grandis Kernel Oil Alleviates Loperamide‐Induced Slow Transit Constipation via Up‐Regulating the Colonic Expressions of Occludin/Claudin‐1/ZO‐1 and 5‐HT3R/5‐HT4R in BALB/c Mice

Wang,

Guo,

et al. 2023

Molecular Nutrition Food Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Studies suggest that elevated expression of ACCα mRNA promotes the deposition of UFAs, and the accumulation of UFAs inhibits the expression of CPT1B mRNA ( Zhu et al, 2022 ). Meanwhile, the main function of SREBP-1c is to promote the synthesis of fatty acids and TG, exerting a positive regulatory effect on adipose deposition and fatty acid metabolism ( Guo et al, 2023 ). In this study, the expression levels of AMPKα2 mRNA and CPT1B mRNA were negatively correlated with IMF, C18:3n6, C20:3n6, and C20:4n6, while the expression levels of ACCα mRNA were positively correlated with their contents.…”

Section: Discussionmentioning

confidence: 99%

Probiotics Increase Intramuscular Fat and Improve the Composition of Fatty Acids in Sunit Sheep through the Adenosine 5′-Monophosphate-Activated Protein Kinase (AMPK) Signaling Pathway

Zhang¹,

Yao²,

Huang³

et al. 2023

Food Sci Anim Resour

View full text Add to dashboard Cite

This experiment aims to investigate the impact of probiotic feed on growth performance, carcass traits, plasma lipid biochemical parameters, intramuscular fat and triglyceride content, fatty acid composition, mRNA expression levels of genes related to lipid metabolism, and the activity of the enzyme in Sunit sheep. In this experiment, 12 of 96 randomly selected Sunit sheep were assigned to receive the basic diet or the basic diet supplemented with probiotics. The results showed that supplementation with probiotics significantly increased the loin eye area, and decreased plasma triglycerides and free fatty acids, increasing the content of intramuscular fat and triglycerides in the muscle and improving the composition of the fatty acids. The inclusion of probiotics in the diet reduced the expression of adenosine 5’-monophosphate-activated protein kinase alpha 2 (AMPKα2) mRNA and carnitine palmitoyltransferase 1B (CPT1B) mRNA, while increasing the expression of acetyl-CoA carboxylase alpha (ACCα) mRNA, sterol regulatory element-binding protein-1c (SREBP-1c) mRNA, fatty acid synthase mRNA, and stearoyl-CoA desaturase 1 mRNA. The results of this study indicate that supplementation with probiotics can regulate fat deposition and improves the composition of fatty acids in Sunit sheep through the signaling pathways AMPK-ACC-CPT1B and AMPK-SREBP-1c. This regulatory mechanism leads to an increase in intramuscular fat content, a restructuring of muscle composition of the fatty acids, and an enhancement of the nutritional value of meat. These findings contribute to a better understanding of the food science of animal resources and provide valuable references for the production of meat of higher nutritional value.

show abstract

“…Кроме того, липолиз жировой ткани частично оказывал этиологическое воздействие на вызванный алкоголем стеатоз печени при предварительном поступлении повышенного уровня МНЖК. Авторы заключили, что повышенный уровень МНЖК усугублял дисфункцию печени, вызванную алкоголем, in vivo [20]. Таким образом, повышенные уровни МНЖК, выявленные в настоящем исследовании, могут быть дополнительными факторами прогрессирования АЖБП.…”

Section: Liver Diseasesunclassified

Features of the fatty acid profile of erythrocyte membranes in patients with fatty liver disease of alcoholic genesis

Kruchinina,

Parulikova,

Belkovets

et al. 2023

Medicinskij sovet

View full text Add to dashboard Cite

Introduction. Alcoholic steatosis, which is a reversible condition, is currently considered a significant risk factor for the progression of diffuse liver pathology, therefore understanding of its mechanisms at the molecular level is essential.Aim. To study the features of the fatty acid profile of erythrocyte membranes in patients with fatty liver disease of alcoholic origin for possible use of fatty acids (FAs) as biomarkers and potential therapeutic targets.Materials and methods. A total of 31 men with alcoholic fatty liver disease (AFLD) (average age of 45.1 ± 17.1 years) and 28 men of comparable age without AFLD and symptomatic pathology of internal organs were examined. The FA composition and levels of erythrocyte membranes (ER) were studied using Agilent 7000B (USA) triple quadrupole gas chromatography/mass spectrometry.Results and discussion. A higher level of a range of saturated FAs (lauric, margaric, pentadecane), monounsaturated FAs (MUFAs), which are additional factors for the progression of AFLD (palmitoleic, total monounsaturated acids), n-6/n-3 polyun-saturated FAs ratio (PUFAs), alpha-linolenic FA was detected in patients with AFL vs the control group (p = 0.00002–0.05). In contrast, the levels of arachidic and docosahexaenoic acids, total eicosapentaenoic and docosahexaenoic n-3 PUFAs, and total n-3 PUFAs were lower in patients with AFLD than in healthy men (p = 0.003–0.01), which is associated with increased ethanol induced adipose tissue lipolysis via PDE3B-AMPK axis. The use of FAs panel (C16:1;9, sum MUFA, n-6/n-3 PUFA, C22:6n3, C20:0) to distinguish patients with AFLD from healthy ones ensured high levels of sensitivity (79%), and specificity (81%) (AUC 0.808). Multidirectional associations of FA levels in erythrocyte membranes with each other and liver tests and lipid profile results were revealed.Conclusion. Thus, the features of erythrocytes membrane FAs in patients with AFLD and the potential to use them as biomarkers for differentiation of people with AFLD from healthy individuals have been identified.

show abstract

Monounsaturated fatty acid-enriched olive oil exacerbates chronic alcohol-induced hepatic steatosis and liver injury in C57BL/6J mice

Abstract: Dietary oil composition determines the pathological process of alcoholic fatty liver disease (AFLD). Oil rich in saturated fatty acids protects whereas oil rich in polyunsaturated fatty acids aggravates alcohol-induced liver...

Cited by 7 publications

References 50 publications

Torreya grandis Kernel Oil Alleviates Loperamide‐Induced Slow Transit Constipation via Up‐Regulating the Colonic Expressions of Occludin/Claudin‐1/ZO‐1 and 5‐HT3R/5‐HT4R in BALB/c Mice

Torreya grandis Kernel Oil Alleviates Loperamide‐Induced Slow Transit Constipation via Up‐Regulating the Colonic Expressions of Occludin/Claudin‐1/ZO‐1 and 5‐HT3R/5‐HT4R in BALB/c Mice

Probiotics Increase Intramuscular Fat and Improve the Composition of Fatty Acids in Sunit Sheep through the Adenosine 5′-Monophosphate-Activated Protein Kinase (AMPK) Signaling Pathway

Features of the fatty acid profile of erythrocyte membranes in patients with fatty liver disease of alcoholic genesis

Contact Info

Product

Resources

About