Myopia (nearsightedness) and presbyopia (aging eye) are the most common refractive errors of the human eye. Technology has advanced toward correcting aberration using various surgical procedures, including laser surgery, as well as prescribing corrective lenses. Hence, the effect of various laser wavelengths on the eye has been extensively studied over the last few decades. Usually, excimer lasers are used for this purpose, which increases the cost of the procedure because they are unique and difficult to manufacture and require regular maintenance. Due to the absorption properties, visible wavelengths do not interact with the corneal layers and hence are currently not used for eye surgery. This study presents the first clinical evidence that a 532 nm laser in combination with an eye-safe fluorescein dye that is in wide clinical use in ophthalmology can be utilized for high-precision ablation purposes due to the photochemical reaction that occurs on an ex vivo porcine eye. Our results show the promise of utilizing inexpensive visible wavelength lasers in the ablation of biological tissues, reducing the high costs of ophthalmological surgical procedures, as well as in other applications.