Monozygotic cleavage polyembryogenesis and conifer tree improvement

Durzan, D. J.

doi:10.3103/s0095452708030031

Cited by 12 publications

(12 citation statements)

References 56 publications

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“…10) [35]. Residual chromatin strands, having anti PCNA fluorescence, were observed during enucleation and before the transi tion of Et cells to embryonal suspensors (Figs 8,9,(15)(16)(17).…”

Section: Axial Tier Cytochemistry and Sds Pagementioning

confidence: 99%

“…Protodermal cells of embry onal initials produce NO from arginine and oxy gen by a putative nitric oxide synthase (NOS) [17,22]. Low levels of NO establish apical distal polar ity and orient plant roots to gravity [39].…”

Section: Axial Tier Cytochemistry and Sds Pagementioning

confidence: 99%

“…Soluble proteins, separated and assayed on gels by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) [44], were reacted with Stains all for comparisons with proteins in cells along the axial tier and released into the culture medium. Control over basal plan development is aimed at improving the quality, uniformity and clonal scale up of elite Norway spruce genotypes for tree improvement programs employing MCP [17]. Modifying the basal plan with a protamine aims at silencing development for the initiation of gamete forma tion from somatic cells in tree breeding programs and for the study of mechanisms contributing to the alternation of generations.…”

Section: Salmine An Arginine Rich Protamine Is Explored For Its Conmentioning

confidence: 99%

“…Cellular autofluorescence at ex 365 nm, em 420 nm was weak. Acetocarmine staining 115 [64], which distinguishes chromoso mal constituents from other organelles, was used as a red 116 orange marker for totipotent cells having the potential for MCP [15,17]. Cationic Stains all [31,32] at pH 4.1 to 4.3 was used to locate proteins along the axial tier of early embryos.…”

Section: Salmine An Arginine Rich Protamine Is Explored For Its Conmentioning

confidence: 99%

“…Norway spruce seed embryos were first classified as not showing MCP [9]. MCP was subsequently demonstrated in cell suspension cultures [3,17,33].…”

Section: Salmine An Arginine Rich Protamine Is Explored For Its Conmentioning

confidence: 99%

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Salmine and the homeotic integrity of early embryos of Norway spruce

Durzan

2010

Cytol. Genet.

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Section: Axial Tier Cytochemistry and Sds Pagementioning

confidence: 99%

Section: Axial Tier Cytochemistry and Sds Pagementioning

confidence: 99%

Section: Salmine An Arginine Rich Protamine Is Explored For Its Conmentioning

confidence: 99%

Section: Salmine An Arginine Rich Protamine Is Explored For Its Conmentioning

confidence: 99%

“…Norway spruce seed embryos were first classified as not showing MCP [9]. MCP was subsequently demonstrated in cell suspension cultures [3,17,33].…”

Section: Salmine An Arginine Rich Protamine Is Explored For Its Conmentioning

confidence: 99%

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Salmine and the homeotic integrity of early embryos of Norway spruce

Durzan

2010

Cytol. Genet.

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Conventional Versus Non-Conventional Methods of Propagation of Forest Tree Species: Applications and Limitations

Manohar,

Shukla,

Shahina

et al. 2024

Biotechnological Approaches for Sustaining Forest Trees and Their Products

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Female parthenogenetic apomixis and androsporogenesis in Douglas-fir embryonal initials in an artificial sporangium

Durzan

2011

Sex Plant Reprod

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Control of female parthenogenetic apomixis and androsporogenesis of Douglas-fir embryonal initials was studied using an experimental culture system in which changes in growth condition can mediate changes in cell identity and outcomes. This culture system constitutes an artificial sporangium in which myriad culture conditions can be simulated and should be applicable for research on a variety of gymnosperms. In this study, embryonal initials from developing seeds from two Douglas-fir trees were rescued and became reprogrammed for female parthenogenetic apomixis (fPA) and parthenogenetic androsporogenesis (mPA). Female PA was initiated by endomitosis forming a binucleate cell with a diploid egg-equivalent and an apoptotic ventral canal nucleus in an archegonial tube. Egg-equivalent nuclei formed cells (parthenotes) that were discharged into an aqueous culture medium. Parthenotes developed axial tiers atypical of early embryogenesis in seeds. Earlier in the year, androsporangial tubes were parthenogenetically differentiated and released monads, dyads, triads, and tetrads into the culture medium. Spores showed chromosomal aberrations. PA demonstrated a temporal separation in gender expression (dichogamy). Embryonal initials brought forward and by-passed the long juvenile phases normally needed for cells to develop into trees and express reproductive maturity. Expressions of fPA and mPA indicated that the specialized culture flasks served as an artificial sporangium (AS). Awareness is raised for the value of an AS for research in gymnosperm life cycles and as a teaching and research laboratory.

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Monozygotic cleavage polyembryogenesis and conifer tree improvement

Cited by 12 publications

References 56 publications

Salmine and the homeotic integrity of early embryos of Norway spruce

Salmine and the homeotic integrity of early embryos of Norway spruce

Conventional Versus Non-Conventional Methods of Propagation of Forest Tree Species: Applications and Limitations

Female parthenogenetic apomixis and androsporogenesis in Douglas-fir embryonal initials in an artificial sporangium

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