Montane Tadpoles in Madagascar: Molecular Identification and Description of the Larval Stages of Mantidactylus elegans, Mantidactylus madecassus, and Boophis laurenti from the Andringitra Massif

Thomas, Meike; Raharivololoniaina, Liliane; Glaw, Frank; Vences, Miguel; Vieites, David R.

doi:10.1643/ch-03-293r2

Cited by 43 publications

(34 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From an ongoing project involving the large-scale identification of tadpoles of Madagascan frogs [5] we here provide data from larval and adult frog species from two sites of high anuran diversity in eastern Madagascar, Andasibe and Ranomafana. These two localities are separated by a geographical distance of ca.…”

Section: Resultsmentioning

confidence: 99%

“…Among the promising applications of this method are the assignments of unknown life-history stages to adult organisms [4,5], the large-scale identification of organisms in ecological or genomic studies [1,6] and, most controversially, explorative studies to discover potentially undescribed "candidate" species [4,7,8]. Although it is not a fundamentally new technique [9], DNA barcoding is promising because technical progress has made its large-scale, automated application feasible [3,6] which may accelerate taxonomic progress [10].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians

et al. 2005

Self Cite

View full text Add to dashboard Cite

BackgroundIdentifying species of organisms by short sequences of DNA has been in the center of ongoing discussions under the terms DNA barcoding or DNA taxonomy. A C-terminal fragment of the mitochondrial gene for cytochrome oxidase subunit I (COI) has been proposed as universal marker for this purpose among animals.ResultsHerein we present experimental evidence that the mitochondrial 16S rRNA gene fulfills the requirements for a universal DNA barcoding marker in amphibians. In terms of universality of priming sites and identification of major vertebrate clades the studied 16S fragment is superior to COI. Amplification success was 100% for 16S in a subset of fresh and well-preserved samples of Madagascan frogs, while various combination of COI primers had lower success rates.COI priming sites showed high variability among amphibians both at the level of groups and closely related species, whereas 16S priming sites were highly conserved among vertebrates. Interspecific pairwise 16S divergences in a test group of Madagascan frogs were at a level suitable for assignment of larval stages to species (1–17%), with low degrees of pairwise haplotype divergence within populations (0–1%).ConclusionWe strongly advocate the use of 16S rRNA as standard DNA barcoding marker for vertebrates to complement COI, especially if samples a priori could belong to various phylogenetically distant taxa and false negatives would constitute a major problem.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians

et al. 2005

Self Cite

View full text Add to dashboard Cite

show abstract

“…2006, 2007), linking adult with juvenile (Greenstone et al . 2005; Thomas et al . 2005; Raharivololoniainaa et al .…”

Section: Introductionmentioning

confidence: 99%

DNA BARCODING: CO1 DNA barcoding amphibians: take the chance, meet the challenge

Smith

Poyarkov

Hebert

2008

Molecular Ecology Resources

168

144

View full text Add to dashboard Cite

Although a mitochondrial DNA barcode has been shown to be of great utility for species identification and discovery in an increasing number of diverse taxa, caution has been urged with its application to one of the most taxonomically diverse vertebrate groups - the amphibians. Here, we test three of the perceived shortcomings of a CO1 DNA barcode's utility with a group of Holarctic amphibians: primer fit, sequence variability and overlapping intra- and interspecific variability. We found that although the CO1 DNA barcode priming regions were variable, we were able to reliably amplify a CO1 fragment from degenerate primers and primers with G-C residues at the 3' end. Any overlap between intra- and interspecific variation in our taxonomic sampling was due to introgressive hybridization (Bufo/Anaxyrus), complex genetics (Ambystoma) or incomplete taxonomy (Triturus). Rates of hybridization and species discovery are not expected to be greater for amphibians than for other vertebrate groups, and thus problems with the utility of using a single mitochondrial gene for species identification will not be specific to amphibians. Therefore, we conclude that there is greater potential for a CO1 barcode's use with amphibians than has been reported to date. A large-scale effort to barcode the amphibians of the world, using the same primary barcode region of CO1, will yield important findings for science and conservation.

show abstract

“…However descriptions of their larval counterparts are few. Tadpoles are an important stage in the amphibian life history and detailed studies of tadpoles can be useful in understanding phylogeny (Haas 2003), natural history (Thomas et al 2005) and evolution (Roelants et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Morphology, natural history and molecular identification of tadpoles of three endemic frog species ofNyctibatrachusBoulenger, 1882 (Anura: Nyctibatrachidae) from Central Western Ghats, India

Priti

Gururaja

Ravikanth

2015

Journal of Natural History

View full text Add to dashboard Cite

Western Ghats of India is known for its high amphibian diversity, but very little is known about their tadpoles. Here, for the first time, we describe tadpoles of three species of an endemic genus, Nyctibatrachus, namely N. kempholeyensis, N. jog and N. kumbara using morphology and molecular techniques. Tadpoles were found in the streams and Myristica swamps of evergreen forests of central Western Ghats. They have a robust body, complete marginal papillae and lack keratodonts. The morphology indicates that these tadpoles are adapted to lotic habitats. In recent years, many of the streams these tadpoles inhabit have been diverted for agriculture and areca plantations. The descriptions of these tadpoles bear relevance for their conservation and can help in understanding amphibian larval ecology.

show abstract

Montane Tadpoles in Madagascar: Molecular Identification and Description of the Larval Stages of Mantidactylus elegans, Mantidactylus madecassus, and Boophis laurenti from the Andringitra Massif

Cited by 43 publications

References 11 publications

Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians

Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians

DNA BARCODING: CO1 DNA barcoding amphibians: take the chance, meet the challenge

Morphology, natural history and molecular identification of tadpoles of three endemic frog species ofNyctibatrachusBoulenger, 1882 (Anura: Nyctibatrachidae) from Central Western Ghats, India

Contact Info

Product

Resources

About