Abstract:Out of focus fluorescence is a dominant limiting factor in the improvement of imaging depth in two-photon fluorescence microscopy. Here we propose a new two-color two-photon fluorescence microscopy scheme, in which two trains of laser pulses with different wavelengths are concentric and complementary. These two beams are overlapped both in space and time only in the focal area. Monte Carlo simulation demonstrates that this scheme can effectively reject out-of-focus fluorescence excitation, and thus has great p… Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.