Monthly Changes in Honey Bee Forewings Estimated Using Geometric Morphometrics

Janczyk, Anna; Tofilski, Adam

doi:10.2478/jas-2021-0002

Cited by 7 publications

(5 citation statements)

References 24 publications

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“…Infestation with ectoparasitic mite Varroa destructor, temperature, cell size, and food availability play an important role regarding wing veins layout [38][39][40][41]. The interaction between all the above-mentioned environmental parameters was shown to affect the seasonal variability in wing venation [42,43].…”

Section: Discussionmentioning

confidence: 99%

Quantifying Abdominal Coloration of Worker Honey Bees

Bubnič,

Prešern

2024

Insects

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The main drawback in using coloration to identify honey bee subspecies is the lack of knowledge regarding genetic background, subjectivity of coloration grading, and the effect of the environment. The aim of our study was to evaluate the effect of environmental temperature on the abdominal coloration of honey bee workers and to develop a tool for quantifying abdominal coloration. We obtained four frames of honey bee brood from two colonies and incubated them at two different temperatures (30 and 34 °C). One colony had workers exhibiting yellow marks on the abdomen, while the other did not. We collected hatched workers and photographed abdomens. Images were analyzed using custom-written R script to obtain vectors that summarize the coloration over the abdomen length in a single value—coloration index. We used UMAP to reduce the dimensions of the vectors and to develop a classification procedure with the support vector machine method. We tested the effect of brood origin and temperature on coloration index with ANOVA. UMAP did not distinguish individual abdomens according to experimental group. The trained classifier sufficiently separated abdomens incubated at different temperatures. We improved the performance by preprocessing data with UMAP. The differences among the mean coloration index values were not significant between the gray groups incubated at different temperatures nor between the yellow groups. However, the differences between the gray and yellow groups were significant, permitting options for application of our tool and the newly developed coloration index. Our results indicate that the environmental temperature in the selected range during development does not seem to impact honey bee coloration significantly. The developed color-recording protocol and statistical analysis provide useful tools for quantifying abdominal coloration in honey bees.

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Section: Discussionmentioning

confidence: 99%

Quantifying Abdominal Coloration of Worker Honey Bees

Bubnič,

Prešern

2024

Insects

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“…The Kazakh population of honey bees of the A.m. carnica breed is currently being bred in six natural and climate zones of Kazakhstan (Limanskaya et al, 2021). Alpatov proposed to use only one cubital index for the assessment and identification of bees (Janczyk and Tofilski, 2021), while other scientists published that for a complete assessment, it is necessary to use all three listed indices and the tarsal index (Ilyasov, 2016).…”

Section: Discussionmentioning

confidence: 99%

Climate Zones and Morphometric Parameters of Apis mellifera carnica Bees

Sheralieva,

Nuraliyeva,

Karymsakov

et al. 2023

American Journal of Animal and Veterinary Sciences

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The Apis mellifera carnica is the most prevalent bee population distributed in the different climate zones of Kazakhstan. In this study, ten bee colonies of the A.m. carnica breed from six bee farms were selected for research, from six climate zones: Desert, semi-desert, steppe, forest-steppe, foothill, and mountain, and different regions: Southern, northern, eastern, western Kazakhstan. During the period of full activity, 30 samples of the right front wings, legs, proboscis, hooks, sternite, and tergite were taken from each bee family. The obtained experimental material was processed by methods of variational statistics (variational series, arithmetic mean, arithmetic mean error, Coefficient of Variation (CV), and correlation coefficient). The results revealed that the highest value of the cubital index was found in bee samples living in a semi-desert, while no significant differences were recorded between the dumbbell index indicators of the bee samples from different zones. The discoidal displacement value was the highest in bees of the semi-desert. A significant (P<0.05) difference was established between the indicators of this trait in bees in forest-steppe, foothill, and mountain zones. According to the development of exterior features, the bee populations were divided into 4 zones. Thus, the highest proboscis length and sternite length and width were found in bees of the foothill. The highest tergite width and length, wax mirror length and width, and the number of hooks were in the bees of the steppe zone. The tarsal index was developed in bees living in the mountain zone and the length and width of the right wing were developed in bees living in the forest-steppe zone. The acquired abilities can be inherited in the future, which will allow the creation of new ecotypes through breeding work.

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“…The morphological characteristics of honeybees are important in the identification and discrimination of honeybee subspecies (Tofilski 2008;Abou-Shaara 2013;Henriques et al 2020;Abed et al 2021;Masaquiza et al 2023). Wing size depends not only on genetics but also on environmental conditions (Janczyk et al 2021;Aglagane et al 2022). The centroid size of the investigated samples was between 6.75 and 6.79, whereas the identified A. m. carnica centroid size was between 6.74-6.76.…”

Section: Discussionmentioning

confidence: 99%

“…Bouga et al (2011) state that all identification methods for bee subspecies do not differ in the accuracy of research results. Several scientists (Nedić et al 2014;Janczyk et al 2021;Prabucki et al 2022) noted that the Cubital index (Ci) and traditional methods were suitable for measuring and distinguishing subspecies in small quantities.…”

Section: Introductionmentioning

confidence: 99%

Geometric morphometric characteristics of Apis mellifera honeybee in Kazakhstan

NURALIEVA,

TAJIYEV,

SHERALIEVA

et al. 2023

Biodiversitas

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Abstract. Nuralieva U, Tajiyev K, Sheralieva Z, Toishimanov M, Moldakhmetova G, Temirbayeva K, Tajieva A. 2023. Geometric morphometric characteristics of Apis mellifera honeybee in Kazakhstan. Biodiversitas 24: 4402-4409. This study was conducted in the whole beekeeping region of Kazakhstan. We researched morphological studies using (45 colony bees in 5 main regions in various geographical and climatic zone environmental conditions. The obtained data were compared with the Apis mellifera carnica reference samples, Apis mellifera caucasica, and Apis mellifera mellifera. Morphometric identification was carried out using 19 landmarks by IdentiFly software. Almost all colonies showed belonging to the lineage C (about 85%), by subspecies to A. m. carnica, A. m. caucasica showed 11%, A. m. mellifera belonged to only 4%. Furthermore, using multivariate principal component analysis compared honeybee lineages between reference samples. There were significant differences in wing landmarks between lineages and subspecies (Mahalanobis square distance). By Mahalanobis, distances between the evolutionary lineages M differ from line C by 15.93 and from line O by 19.03 units. In contrast, the distance between lines C and O is insignificant, with satisfactory reliability. The forewing wing size evaluated centroid size, which showed the highest degree of honeybees in the west and south Kazakhstan zones. The percentage of variation in wing centroids was relatively small between analyzed samples (less than 1%) but extremely changed for reference samples A. m. carnica, A. m. caucasica, A. m. mellifera, where log centroid sizes showed 1.33%, 4.18%, and 4.67%, respectively. The results showed significant differences between populations in different climatic zones.

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Monthly Changes in Honey Bee Forewings Estimated Using Geometric Morphometrics

Cited by 7 publications

References 24 publications

Quantifying Abdominal Coloration of Worker Honey Bees

Quantifying Abdominal Coloration of Worker Honey Bees

Climate Zones and Morphometric Parameters of Apis mellifera carnica Bees

Geometric morphometric characteristics of Apis mellifera honeybee in Kazakhstan

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